C-terminal hybrid mutant of Bacillus pumilus cyanide dihydratase dramatically enhances thermal stability and pH tolerance by reinforcing oligomerization

Autor: Crum, M A, Park, J M, Sewell, B T, Benedik M J

Publikováno v: Journal of Applied Microbiology

Aims: To investigate the impact of the highly variable C-terminal domain of cyanide dihydratase, a member of the nitrilase superfamily, on its activity and stability. Methods and Results: Generating and analysing the thermal stability and pH toleranc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3158::a04a4eed10a55f432510b7fbc11ff6db
http://hdl.handle.net/11427/21659

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Modification of nitrilase based on computer screening and efficient biosynthesis of 4-cyanobenzoic acid.

Autor: Jin YZ; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China.; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.; Zhejiang Yongtai Technology Co., Ltd., Linhai, Zhejiang, China., Shen JD; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China.; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China., Wang YX; Princeton International School of Mathematics and Science, Princeton, New Jersey, USA., Shao HM; Zhejiang Yongtai Technology Co., Ltd., Linhai, Zhejiang, China., Xia HJ; Zhejiang Yongtai Technology Co., Ltd., Linhai, Zhejiang, China., Lin JH; Zhejiang Yongtai Technology Co., Ltd., Linhai, Zhejiang, China., Liu ZQ; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China.; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China., Zheng YG; The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, China.; Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.

Publikováno v: Biotechnology journal [Biotechnol J] 2024 Mar; Vol. 19 (3), pp. e2300706.

Location and Orientation of the Genetic Toxin–Antitoxin Element hok/sok in the Plasmid Affect Expression of Pharmaceutically Significant Proteins in Bacterial Cells.

Autor: Khodak, Yulia A.¹ (AUTHOR), Shaifutdinov, Rolan R.¹ (AUTHOR), Khasanov, Danila S.¹ (AUTHOR), Orlova, Nadezhda A.¹ (AUTHOR), Vorobiev, Ivan I.¹ (AUTHOR) ptichman@gmail.com

Publikováno v: Biochemistry (00062979). Sep2023, Vol. 88 Issue 9, p1326-1337. 12p.