Zobrazeno 1 - 10
of 120
pro vyhledávání: '"B.A. Hart"'
Publikováno v:
Toxicology. 184:157-178
This study demonstrates that in vitro exposure of adult rat alveolar epithelial cells to CdCl(2) decreases DNA binding activity of specificity protein 1 (Sp1), a zinc-finger transcription factor known to play a key role in eukaryotic gene expression,
Publikováno v:
Toxicology. 160:65-70
This review article discusses the major cellular and molecular responses characterizing pulmonary adaptation to cadmium (Cd) that may ultimately contribute to Cd carcinogenesis. Hallmarks of Cd adaptation include hyperplasia and hypertrophy of type I
Publikováno v:
Toxicology. 161:25-38
This study evaluated the effects of cadmium (Cd) adaptation in cultured alveolar epithelial cells on oxidant-induced DNA damage and its subsequent repair. Using the comet assay, we determined that lower levels of DNA damage occurred in Cd-adapted cel
Publikováno v:
Toxicology. 151:45-54
The controlled step in de novo glutathione (GSH) synthesis is catalyzed by gamma-glutamylcysteine synthetase (gamma-GCS), a dimeric enzyme consisting of a heavy catalytic subunit (gamma-GCS-HS) and a light regulatory subunit (gamma-GCS-LS). We have p
Publikováno v:
Cell Biology and Toxicology. 16:347-362
Exposure of rat alveolar epithelial cells to 10 μmol/L CdCl2 causes time-dependent increases in steady-state mRNA levels of the γ-glutamylcysteine synthetase catalytic (heavy) subunit (γ-GCS) and of glutathione S-transferase isoforms (GST-α and G
Publikováno v:
Toxicology. 133:43-58
The mode of cadmium-induced cell death was investigated in a rat lung epithelial cell line. Cells, grown to near confluence, were exposed to 0-30 microM CdCl2 for 0-72 h. Phase contrast microscopy and fluorescent nuclear staining showed that Cd cause
Publikováno v:
Toxicology. 112:205-218
This study examined the expression of metallothionein (MT) isoforms in the lungs of Lewis rats exposed to Cadmium (Cd) aerosols. With the use of isoform-specific oligonucleotide probes and Northern hybridization analysis, we demonstrated that a drama
Publikováno v:
Toxicology Letters. 81:131-139
Alveolar epithelial type II cells (AEIIC) were isolated from male Lewis rats following repeated in vivo cadmium aerosol exposure and were subsequently evaluated for their oxidant resistance in vitro. AEIIC from Cd-adapted animals removed a greater pr
Publikováno v:
Toxicology. 98:1-13
Metallothionein (MT) is a metal and thiol-rich protein readily induced by cadmium (Cd) exposure. In vitro experiments have demonstrated that MT is able to serve as a scavenger of hydroxyl radicals as well as superoxide anions, albeit to a lesser exte
Publikováno v:
Cancer Letters. 75:121-128
Eleven pairs of surgically resected lung cancers and corresponding non-neoplastic lung tissue were evaluated for metallothionein (MT) and metal content (cadmium and copper) by the heme/109Cd binding assay and atomic absorption spectroscopy, respectiv