Activity-Based Protein Profiling (ABPP) for cellular deubiquitinase (DUB) and inhibitor profiling at deep and high-throughput levels

Autor: Hannah B. L. Jones, Raphael Heilig, Benedikt M. Kessler, Adán Pinto-Fernández

Publikováno v: Methods in Molecular Biology ISBN: 9781071628027

This chapter provides detailed methodology and materials required to profile deubiquitinases (DUBs) in a cellular matrix using specific activity-based probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of act

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecbd745016e63019e6f2bc4d38fa0875
https://ora.ox.ac.uk/objects/uuid:e0193863-2c3c-40a3-83cb-33e8ea9a0f86

Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes

Autor: Vickery L. Arcus, Sivaraman Subramanian, Hannah B. L. Jones, Marc W. van der Kamp, Christopher R. Pudney, Frank Vollmer, Samuel Winter, Simona Frustaci

Publikováno v: et al. 2021, ' Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes ', ACS Applied Nano Materials, vol. 4, no. 5 . https://doi.org/10.1021/acsanm.1c00176
ACS Applied Nano Materials

Here, we report a label-free gold nanoparticle-based single-molecule optical platform to study the immobilization, activity, and thermodynamics of single enzymes. The sensor uses plasmonic gold nanoparticles coupled to optical whispering gallery mode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12f249d84cbfce55f90a194c67afcb66
https://doi.org/10.1021/acsanm.1c00176

Ultrasound Sector Scanning Used to Define Changes in Muscle Configuration

Autor: R J Saltzstein, J V Danoff, T H Shawker, B L Jones, L Gerber

Publikováno v: Journal of Rehabilitation Medicine. 21:209-212

Dynamic configurational changes in the rectus femoris muscle were examined using a real time ultrasound sector scanner in ten normal subjects. The angle of passive knee flexion was varied as transverse ultrasound scanning was performed at the mid-tra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4b6c2232cb929321d33b11d8aef59473
https://doi.org/10.2340/165019778921209212

Chemical Mapping Exposes the Importance of Active Site Interactions in Governing the Temperature Dependence of Enzyme Turnover

Autor: Vickery L. Arcus, Samuel Winter, Christopher R. Pudney, Erica J. Prentice, Dora M. Răsădean, Hannah B. L. Jones, Michael J. Danson, G. Dan Pantoş, Rory M. Crean, Gergely Katona, Marc W. van der Kamp

Publikováno v: Winter, S, Jones, H B L, Răsădean, D M, Crean, R M, Van der Kamp, M W & Pudney, C R 2021, ' Chemical Mapping Exposes the Importance of Active Site Interactions in Governing the Temperature Dependence of Enzyme Turnover ', ACS Catalysis, vol. 11, no. 24, pp. 14854-14863 . https://doi.org/10.1021/acscatal.1c04679
ACS Catalysis

Uncovering the role of global protein dynamics in enzyme turnover is needed to fully understand enzyme catalysis. Recently, we have demonstrated that the heat capacity of catalysis, ΔCP‡, can reveal links between the protein free energy landscape,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::190ba99b0f95305e563e8bbbec3a3647
https://research-information.bris.ac.uk/en/publications/5ae0ae7e-e25c-499a-8349-e605a8e52e02

The Solar Orbiter EUI instrument: The Extreme Ultraviolet Imager

Autor: J. Hansotte, R. Müller, M. Hailey, P. Schlatter, A. Lawrenson, M. Monecke, Hardi Peter, Julien Rosin, Koen Stegen, Jean-Claude Vial, S. Parenti, Ali BenMoussa, Etienne Renotte, N. Szwec, Marie-Laure Hellin, R. Enge, M. Haberreiter, A. Philippon, P. Coker, B. Giordanengo, L. Dolla, K. Heerlein, Werner Schmutz, Matthew J. West, K. Ruane, J.-F. Hochedez, M. Chaigneau, M. Kahle, Emmanuel Mazy, J. Scanlan, J. Tandy, J. Barbay, B. Chares, T. Appourchaux, Laurence Rossi, C. Dumesnil, E. Pylyser, R. Aznar Cuadrado, Louise K. Harra, S. Meyer, F. Cabé, Benoit Marquet, Evgueni Meltchakov, Andrei Zhukov, D. Westwood, Bogdan Nicula, G. Willis, M.-F. Ravet-Krill, B. Borgo, S. Hemsley, Isabelle Tychon, N. Guerreiro, D. Linder, R. Mercier, Sylvie Liébecq, Thomas Wiegelmann, Philip J. Smith, M. Gyo, L. Mountney, G. Davison, Frédéric Rabecki, Jean-Marie Gillis, P. Langer, P. Roth, Sami K. Solanki, Luciano Rodriguez, Jean-Philippe Halain, L. Cadiergues, P. Addison, V. Büchel, D. Markiewicz-Innes, X. Zhang, Daniel Pfiffner, M. Roulliay, Antoine Rousseau, Stéphane Roose, Udo Schühle, M. Spescha, Jean-Yves Plesseria, Lionel Jacques, J. C. Le Clec’h, Yvan Stockman, G. Del Zanna, T. Kennedy, C. Beurthe, B. Mampaey, Franck Delmotte, C. Theobald, C. Choque Cortez, S. Meining, S. François, David Long, M. Bergmann, F. Rouesnel, S. Koller, C. Tamiatto, M. Bouzit, David Berghmans, D. Bates, L. Bradley, E. Kotsialos, A. Hafiz, D. Davies, J. B. L. Jones, S. Smit, Véronique Delouille, Gilles Morinaud, Aline Hermans, S. Coumar, J. Rebellato, Yvette Houbrechts, Stephan Werner, Werner Curdt, J. Cutler, K. Bonte, Daniel B. Seaton, Jean-Marc Defise, F. Moron, M. Klaproth, Eric Buchlin, J.-J. Fourmond, I. Phillips, Jörg Büchner, Pierre Rochus, M. Kolleck, Christophe Hecquet, Sarah A. Matthews, L. van Driel-Gesztelyi, Lucie M. Green, Cis Verbeeck, S. Gissot, F. Dürig, M. Condamin, Cedric Lenaerts, F. Auchère, K. Ihsan, K. Silliman, A. Guilbaud, Luca Teriaca, E. Kraaikamp, F. Monfort, A. Jérôme, Andreas Zerr, E. Marsch, Berend Winter, D. N. Baker, Alexandra Mazzoli, A. Spencer, V. Hervier

Publikováno v: Astronomy and Astrophysics-A&A
Astronomy and Astrophysics-A&A, EDP Sciences, 2020, 642, pp.A8. ⟨10.1051/0004-6361/201936663⟩
Astronomy and Astrophysics-A&A, EDP Sciences, 2020, 642, ⟨10.1051/0004-6361/201936663⟩

Context.The Extreme Ultraviolet Imager (EUI) is part of the remote sensing instrument package of the ESA/NASA Solar Orbiter mission that will explore the inner heliosphere and observe the Sun from vantage points close to the Sun and out of the eclipt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fde6c949827de1c6d33fe569ab239b5
https://hal.archives-ouvertes.fr/hal-02991327