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Cyclophilin Isoforms from Digitalis lanata. Sequences and Expression During Embryogenesis and Stress
Publikováno v:
Journal of Plant Physiology. 155:212-219
Summary Two cyclophilin (CyP) nucleotide sequences (DLCYCLO cDNA and DLCYP 18 genomic DNA) from Digitalis lanata are reported. The 765 by DLCYCLO cDNA contains an open reading frame of 516 nucleotides encoding a protein of 172 amino acids with a mole
Publikováno v:
Phytochemistry. 51:357-361
Cuscuta europaea, C. platyloba and C. reflexa were cultivated on stems of Digitalis lanata und D. purpurea in the second year of vegetation as well as on petioles of rosettes of D. purpurea in the first year of vegetation. C. reflexa was most suitabl
Publikováno v:
Phytochemistry. 49:71-77
Crown galls developing on leaves and stems of Digitalis lanata plants grown in the greenhouse contained digitalinum verum, glucodigifucoside, deacetyllanatoside C and verodoxin as the main cardenolides. Crown galls formed on leaves of axenic plants a
Publikováno v:
Botanica Acta. 111:22-27
Digitalis lanata was transformed by agrobacteria-mediated gene transfer with a chimeric reporter gene encoding for β-glucuronidase (CUS) from Escherichia coll under the control of the plastocyanin 3 (Pc3) promoter from Spinada oleracea (Pc3::uidA fu
Publikováno v:
Journal of Plant Physiology. 151:209-215
Summary Pieces of leaves of axenically grown shoots and plants of Digitalis lanata were infected with different wild-type strains of Agrobacterium rhizogenes. Hairy roots were formed with only 3 strains, all wide host range agropine-type agrobacteria
Publikováno v:
Journal of Plant Physiology. 147:53-57
Summary Leaf discs of axenic ally grown Digitalis lanata plants as well as protoplasts of the embryogenic D. lanata strain VII were transformed with Agrobacterium tumefaciens strain C58CI using the integration vector pGV 3850::1103 neo and the binary
Publikováno v:
Biochemie und Physiologie der Pflanzen. 188:23-31
Summary In-vitro-grown shoots and clone plants of Digitalis lanata showed high rates of transpiration if placed in dry air. The easy loss of water was due to morphological and physiological abnormalities. Leaves of in-vitro-grown shoots and plants ha
Publikováno v:
Journal of Plant Physiology. 139:212-217
Summary Treatment of Digitalis lanata cells and protoplasts with osmotic agents as well as slow cooling (0.5 K min-1) to -60°C in an osmotically-active cryoprotector solution resulted in considerable reduction of volume and surface of the protoplast