Zobrazeno 1 - 10
of 16
pro vyhledávání: '"B. D. Efremov"'
Autor:
D. G. Kozlov, S. E. Cheperegin, A. V. Malysheva, B. D. Efremov, I. I. Gubaidullin, E. P. Sannikova
Publikováno v:
Applied Biochemistry and Biotechnology. 193:1351-1364
Phospholipase A2 (PLA2) has found extensive use in industry. However, recombinant PLA2 production in different expression systems is a difficult task because of its toxicity to cell membranes. We report here the development of an effective method for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::80abc0ff80c82c07eb93f011b74a7376
https://doi.org/10.1007/s12010-020-03333-7
https://doi.org/10.1007/s12010-020-03333-7
Publikováno v:
Applied biochemistry and biotechnology. 193(5)
Phospholipase A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::adecf2cb1c42f58edfe3beb3ccf4cd69
https://pubmed.ncbi.nlm.nih.gov/32388605
https://pubmed.ncbi.nlm.nih.gov/32388605
Autor:
E. K. Kotlova, E. P. Sannikova, Vadim S. Pokrovsky, T. S. Kubasova, I. I. Gubaydullin, G. E. Konstantinova, N. V. Bulushova, V. V. Ryabichenko, S. V. Yarotsky, I. A. Zalunin, G. G. Chestukhina, Alexander A. Shtil, B. D. Efremov, S. E. Cheperegin, D. G. Kozlov
Publikováno v:
Molecular Biotechnology. 58:528-539
The modified asparaginase Was79 was derived from the recombinant wild-type L-asparaginase of Wolinella succinogenes. The Was79 contains the amino acid substitutions V23Q and K24T responsible for the resistance to trypsinolysis and the N-terminal hepa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bedb461e2ebc4a06a568f909c3b895f
https://doi.org/10.1007/s12033-016-9950-1
https://doi.org/10.1007/s12033-016-9950-1
Publikováno v:
Applied Biochemistry and Microbiology. 50:829-834
An analytical system for a quantitative assessment of contributions of pre- and pro-components of the yeast α-factor leader to key stages of somatropin secretion in Saccharomyces cerevisiae has been designed. Using this system, it was shown that: 1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::104e2b05d5cd714759ae23ea7eb515f3
https://doi.org/10.1134/s000368381409004x
https://doi.org/10.1134/s000368381409004x
Publikováno v:
Applied Biochemistry and Microbiology. 49:656-659
The dependence of secretion efficiency in Pichia pastoris cells on the copy number of proregions in leader polypeptides has been studied. The humanized light kappa-chain of the murine H3-1 antibody was used as a reporter protein. The leader pre-pro-p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::737074ac14ffd86c052443e3b14ee629
https://doi.org/10.1134/s0003683813070065
https://doi.org/10.1134/s0003683813070065
Publikováno v:
Russian Journal of Genetics. 38:1121-1126
It is known that upon STA2 gene expression in Saccharomyces cerevisiae cells, two transcripts of different lengths are formed. These fragments contain different start codons of translation (AUG1 and AUG2) located in the same reading frame. The ratio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1252f968255bbcf6c24b8a0085e8e5b1
https://doi.org/10.1023/a:1020688301520
https://doi.org/10.1023/a:1020688301520
Publikováno v:
Biotechnology and Bioengineering. 60:492-497
The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1 Km ) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1 Km gene have acquired extracellular inulase act
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b61d64f53a7d36a1fc394da0cc4bff54
https://doi.org/10.1002/(sici)1097-0290(19981120)60:4<492::aid-bit11>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19981120)60:4<492::aid-bit11>3.0.co
Autor:
N. N. Kartasheva, N. I. Sutsov, S. V. Benevolensky, S. I. Ibragimova, B. D. Efremov, D. G. Kozlov
Publikováno v:
Biotechnology and Bioengineering. 46:285-290
A procedure was developed for construction of industrial strains of distiller's yeast (Saccharomyces cerevisiae). It includes several steps: construction of congenic genetically marked haploid strains of opposite mating types starting from an industr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6748ffa248f6e0b8b2819a62815d7961
https://doi.org/10.1002/bit.260460312
https://doi.org/10.1002/bit.260460312
Publikováno v:
Genetika. 38(10)
It is known that upon STA2 gene expression in Saccharomyces cerevisiae cells, two transcripts of different lengths are formed. These fragments contain different start codons of translation (AUG1 and AUG2) located in the same reading frame. The ratio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c87af005aca2bebce96d48750bf771cf
https://pubmed.ncbi.nlm.nih.gov/12449642
https://pubmed.ncbi.nlm.nih.gov/12449642
Publikováno v:
Biotechnology and bioengineering. 60(4)
The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1Km gene have acquired extracellular inulase activi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2d2576ceb607ee4e7545ae3bee5d0550
https://pubmed.ncbi.nlm.nih.gov/10099455
https://pubmed.ncbi.nlm.nih.gov/10099455