Zobrazeno 1 - 4
of 4
pro vyhledávání: '"B N Hay"'
Publikováno v:
BioTechniques. 12(6)
A new method is presented for creating antibody expression libraries in Escherichia coli. Rather than perform two cloning steps to express the heavy and light chains of the antigen binding domain, we have used a fusion-PCR method to link the coding r
Publikováno v:
Human antibodies and hybridomas. 3(2)
We have combined the molecular biology methods of the polymerase chain reaction and recombinant DNA cloning in bacteriophage lambda to express a human IgM Fab in Escherichia coli using genes derived from an Epstein-Barr virus transformed cell line. T
Autor:
H H Hogrefe, M M Kubitz, M Alting-Mees, R L Mullinax, D Ardourel, B N Hay, E A Gross, J R Amberg, Jay M. Short, A Greener
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 87(20)
We have applied a molecular biology approach to the identification of human monoclonal antibodies. Human peripheral blood lymphocyte mRNA was converted to cDNA and a select subset was amplified by the polymerase chain reaction. These products, contai
Autor:
Lakshmi Sastry, Kim D. Janda, Richard A. Lerner, W.D. Huse, B N Hay, Michelle A. Alting-Mees, J.A. Sorge, Jay M. Short, Stephen J. Benkovic
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 86(15)
Efficient generation of catalytic antibodies is uniquely dependent on the exact nature of the binding interactions in the antigen-antibody complex. Current methods for generation of monoclonal antibodies do not efficiently survey the immunological re