Zobrazeno 1 - 10
of 65
pro vyhledávání: '"B N, Il'iashenko"'
Autor:
B N, Il'iashenko
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (2)
Conjugative-like transfer of hybrid plasmid RP4::Mucts62 from Escherichia coli to plasmid-free Bacillus polymyxa was carried out. Bacillus transcipients are detected by the markers of kanamycin and tetracyclin resistance RP4 and thermal sensitivity t
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (3-4)
The plasmid RP4::Mu cts62 in stably inherited by Erwinia carotovora 268 strain. Under the conditions of thermoinduction bacteriophage Mu is segregated and completely eliminated more intensively than in Escherichia coli cells. At thermoinduction the t
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (9)
The plasmid RP4::Mu cts62 is transferred from Escherichia coli cells into a recipient strain Erwinia carotovora 268 by conjugation with the frequency 1.5-5 x 10(-7) per donor cell. The maximal frequencies of transfer are obtained by cultivation of do
Autor:
V S, Britskaia, V V, Danileĭchenko, V I, Kovalishin, P D, Gordiĭ, N A, Pereverzev, N V, Klitsunova, V V, Gosteva, B N, Il'iashenko
Publikováno v:
Mikrobiologicheskii zhurnal. 52(4)
Transfer of conjugative hybrid plasmid RP4::Mu cts 62 from Escherichia coli into Bac. cereus, Bac. thuringiensis, Bac. mesentericus and Bac. polymyxa cells led to the multiple effects on the structure and physiology of bacillus cells. It has resulted
Autor:
N V, Timakova, G I, Aleshkin, I V, Titova, M G, Goderdzishvili, V I, Zakharenko, G V, Brukhanskiĭ, O G, Loginova, S Ia, Ditiatkin, B N, Il'iashenko
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 109(3)
The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited
Publikováno v:
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (5)
Publikováno v:
Genetika. 12(8)
The paper is initiated in the aim to use the effect of freezing-thawing in the system of intact Escherichia coli cells treated with Ca++ ions to increase the transfection efficiency. It is demonstrated that freezing-thawing of Ca++ treated cells incr
Publikováno v:
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (12)
The action of tetracaine hydrochloride, a local anesthetic, on the effectiveness of plasmid transformation and transfection in variants of E. coli K-12 has been studied. The concentrations of tetracaine hydrochloride used in the experiment (0.002 M)
Publikováno v:
Biulleten' eksperimental'noi biologii i meditsiny. 89(6)
The saccharose density gradient (30--55%) centrifugation technique applied to E. coli membrane preparations was used to show that treatment of the bacteria with Ca2+ in the cold results in the redistribution of the absorbed phage DNA from the cell wa
Publikováno v:
Genetika. 14(1)
Some trends of the interaction of the competence factor with the cells are described on the model of phage lambda transfection. It is shown that this process depends on the composition of the recipients growth media, the temperature regime in the int