Protein production and purification

Autor: Adelinda Yee, R Kim, J.M. Sauder, Youngchang Kim, Thomas Acton, Andrzej Joachimiak, J Weigelt, M. Zhou, J Ming, Kim C-Y., B M Hallberg, Tarun Gheyi, Geoffrey S. Waldo, Gaetano T. Montelione, Udo Oppermann, Konrad Büssow, Shane Atwell, Sirano Dhe-Paganon, Anja Schütz, Yoav Peleg, C K Ho, J.S. Emtage, Stephen K. Burley, Kim S-H., John G. Luz, Mark R. Chance, L Zhao, Jaime Prilusky, Renaud Vincentelli, William H. Eschenfeldt, Lucy Stols, Masayori Inouye, Andrej Sali, Shira Albeck, Raymond C. Stevens, Cheryl H. Arrowsmith, L C Ma, Aled M. Edwards, Christian Cambillau, Udo Heinemann, Pär Nordlund, Hung L-W., Orly Dym, F Zhang, Joel L. Sussman, Ritu Shastry, Park H-W., Ian A. Wilson, Susanne Gräslund, Mark I. Donnelly, Jeffrey B. Bonanno, Scott A. Lesley, Andras Fiser, Kristin C. Gunsalus, James E. Bray, Frank R. Collart, M Jiang, Raymond Hui, Ruiying Wu, D Wang, John F. Hunt, Kellie Cunningham, Rong Xiao, Alexei Savchenko, Raymond J. Owens, Hao Wang, Haleema Janjua, Yunyu Shi, Soumya Swaminathan, Kevin Bain, Stefan Knapp, Zihe Rao, Steven C. Almo, David I. Stuart, S Yokoyama, Irina Dementieva, S Daenke, Thomas C. Terwilliger, Opher Gileadi, D.A. Thompson, Liang Tong, Tamar Unger, Stephen Anderson, F.W. Studier

Publikováno v: Nature Methods

In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analys

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