Zobrazeno 1 - 10
of 181
pro vyhledávání: '"B Lesbats"'
Autor:
M. Synguelakis, Françoise Loctin, B. Lesbats, L Eder-Colli, P Corrèges, J Falk-Vairant, N Salem, François-Marie Meunier, Yves Dunant, Maurice Israël
Publikováno v:
Journal of Neurochemistry. 66:1322-1325
Transmitter release was elicited in two ways from cultured cells filled with acetylcholine: (a) in a biochemical assay by successive addition of a calcium ionophore and calcium and (b) electrophysiologically, by electrical stimulation of individual c
Autor:
Maurice Israël, B. Lesbats
Publikováno v:
Journal of Neurochemistry. 67:2624-2627
A bioluminescent GABA assay is described. The principle of the procedure is based on the action of GABASE (GABA-aminotransferase plus succinic semialdehyde dehydrogenase), coupled to the detection of succinic semialdehyde and NADH, using Photobacteri
Autor:
Jean-Léon Tchelingerian, L. Vignais, B. Lesbats, Monique Tomasi, Pierre-Olivier Couraud, Maurice Israël, Jérôme Quinonero
Publikováno v:
Neuropharmacology. 36:1789-1793
By loading cells in culture with acetylcholine (ACh) we have characterized a calcium-dependent release mechanism and shown that it was expressed independently of synthesis or storage of ACh. (Israel et al., 1994, Neurochemistry International 37, 1475
Autor:
J. Stinnakre, P Corrèges, M. Synguelakis, J. Bruner, B. Lesbats, Y. Dunant, Gaultier P, François-Marie Meunier, Frederic A. Meunier, Maurice Israël, J Falk-Vairant
Publikováno v:
Neuroscience. 75:353-360
Neuronal properties such as neurotransmitter uptake and release can be expressed in non-neuronal cells. We show here that fibroblasts—mouse cell line L-M(TK − )—are able to take up acetylcholine from the external medium and to release it in res
Publikováno v:
Pflügers Archiv - European Journal of Physiology. 432:853-858
Treatment with 100 or 250 microM ZnCl2 irreversibly blocked neurotransmission in the Torpedo electric organ by inhibiting acetylcholine (ACh) release. In Zn2+-treated tissue, release failure did not result from impairment of Ca2+ entry since stimulat
Autor:
François-Marie Meunier, M. Synguelakis, N Salem, B Lesbats, P Corrèges, M Israël, J Falk-Vairant, L Eder-Colli, Y Dunant
Publikováno v:
Journal of Neuroscience Research. 45:195-201
Several neuronal and non-neuronal cell lines express a Ca(2+)-dependent mechanism of transmitter release that can be demonstrated after loading the cells with acetylcholine during culture. In contrast, a particular cell line, the neuroblastoma N18TG-
Autor:
E. Roulet, Françoise Loctin, Alain Bloc, M. Synguelakis, B. Lesbats, Yves Dunant, L Eder-Colli, Maurice Israël, J Falk-Vairant, N Salem, François-Marie Meunier, P Corrèges
Publikováno v:
Proceedings of the National Academy of Sciences. 93:5203-5207
Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subun
Publikováno v:
Neurochemistry International. 25:103-109
A procedure is described to fill up cells in culture with ACh and study its calcium dependent release, by-passing the synthesis steps. Whether differentiated or not with dbc-AMP, the NG108-15 cells efficiently released ACh when stimulated with calciu
Publikováno v:
Biology of the Cell. 78:145-154
Mediatophore is a nerve terminal membrane protein purified from Torpedo electric organ on its ability to translocate acetylcholine upon calcium action. An antiserum able to immunoprecipitate mediatophore activity was used to study the subcellular dis
Publikováno v:
Neurochemistry International. 22:53-58
We describe, in the present work, a continuous procedure for measuring the release of glutamate, and have applied it to analyze the co-release of glutamate and acetylcholine from cholinergic nerve terminals of electric organ synaptosomes. The two sub