Výsledky vyhledávání - "B G, de Grooth"

Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers

Autor: M L, Bennink, S H, Leuba, G H, Leno, J, Zlatanova, B G, de Grooth, J, Greve

Publikováno v: Nature structural biology. 8(7)

Single chromatin fibers were assembled directly in the flow cell of an optical tweezers setup. A single lambda phage DNA molecule, suspended between two polystyrene beads, was exposed to a Xenopus laevis egg extract, leading to chromatin assembly wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::76a1c5829cb19b203cb53296429e378b
https://pubmed.ncbi.nlm.nih.gov/11427891

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Cell analysis system based on immunomagnetic cell selection and alignment followed by immunofluorescent analysis using compact disk technologies

Autor: A G, Tibbe, B G, de Grooth, J, Greve, P A, Liberti, G J, Dolan, L W, Terstappen

Publikováno v: Cytometry. 43(1)

Although the flow cytometer has become the standard in cell analysis, it has limitations. Recently, we introduced a new cell analysis method based on immunomagnetic selection and aligning of cells. No flow system is needed and cell analysis can be pe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8c917363ab77305809e80793999b3f5f
https://pubmed.ncbi.nlm.nih.gov/11122482

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Orientation of the chromophore dipoles in the TOTO-DNA system

Autor: J M, Schins, A, Agronskaia, B G, de Grooth, J, Greve

Publikováno v: Cytometry. 37(3)

Flow cytometry has been applied successfully to the sizing of medium to large-sized DNA molecules, thanks to the excellent staining properties of cyanine chromophores such as TOTO (homodimer of thiazole orange) (Petty et al.: Anal Chem 67:1755-1761,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::20cde549bd33d944f4535d5e65e3bc1e
https://pubmed.ncbi.nlm.nih.gov/10520204

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3D single-particle tracking and optical trap measurements on adhesion proteins

Autor: I M, Peters, Y, van Kooyk, S J, van Vliet, B G, de Grooth, C G, Figdor, J, Greve

Publikováno v: Cytometry. 36(3)

A three-dimensional single-particle tracking system was combined with an optical trap to investigate the behavior of transmembrane adhesion proteins. We exploited this setup to investigate which part of the cell adhesion protein LFA-1 forms a connect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::75596675d8a42c52e5f9cab1186aa616
https://pubmed.ncbi.nlm.nih.gov/10404967

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Visualising individual green fluorescent proteins with a near field optical microscope

Autor: M F, Garcia-Parajo, J A, Veerman, G M, Segers-Nolten, B G, de Grooth, J, Greve, N F, van Hulst

Publikováno v: Cytometry. 36(3)

The use of the green fluorescence protein (GFP) as an individual marker for applications in molecular biology requires detailed understanding of its photophysical and photodynamical properties. We investigated individual S65T mutants of GFP both on a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a0414df20c58d47412ed2b3604fe2cdd
https://pubmed.ncbi.nlm.nih.gov/10404974

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Single-molecule manipulation of double-stranded DNA using optical tweezers: interaction studies of DNA with RecA and YOYO-1

Autor: M L, Bennink, O D, Schärer, R, Kanaar, K, Sakata-Sogawa, J M, Schins, J S, Kanger, B G, de Grooth, J, Greve

Publikováno v: Cytometry. 36(3)

By using optical tweezers and a specially designed flow cell with an integrated glass micropipette, we constructed a setup similar to that of Smith et al. (Science 271:795-799, 1996) in which an individual double-stranded DNA (dsDNA) molecule can be

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fd1fb697dedf0fb657ada43cda9e4216
https://pubmed.ncbi.nlm.nih.gov/10404969

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Photon-counting device compatible with conventional flow cytometric data acquisition electronics

Autor: A, Agronskaia, A, Florians, K O, van der Werf, J M, Schins, B G, de Grooth, J, Greve

Publikováno v: Cytometry. 32(3)

We present an electronic scheme that enables us to use a photon-counting device (photomultiplier or avalanche photodetector) for measuring extremely weak signals in a flow cytometer. It can be used as a sole detector, or in combination with other (co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fddced1bcb1342d90966eb203954a1ab
https://pubmed.ncbi.nlm.nih.gov/9667515

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Experimental and model investigations of bleaching and saturation of fluorescence in flow cytometry

Autor: R M, Doornbos, B G, de Grooth, J, Greve

Publikováno v: Cytometry. 29(3)

We investigated the fluorescence emission from three fluorophores commonly used for labeling cells in flow cytometry. We have demonstrated that the fluorescence emission from cells labeled with fluorescein-isothiocyanate (FITC), phycoerythrin (PE), a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::359499015ea764ec0b6ff645abe993d9
https://pubmed.ncbi.nlm.nih.gov/9389437

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Light-induced absorbance changes due to photosystems 1 and 2 in spinach chloroplasts at minus 50 degrees C

Autor: J, Amesz, B G, De Grooth

Publikováno v: Biochimica et biophysica acta. 376(2)

Absorbance changes in the region 500-565 nm and at 702 nm, brought about by excitation of Photosystems 1 and 2, respectively, were measured in spinach chloroplasts at minus 50 degrees C. Either dark-adapted chloroplasts were used or chloroplasts prei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::77a91694bac7629381531cd9402daeb6
https://pubmed.ncbi.nlm.nih.gov/1115780

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