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pro vyhledávání: '"B E, Slatko"'
Autor:
B E, Slatko, L M, Albright
Publikováno v:
Current protocols in molecular biology.
The accuracy of DNA sequence determination depends largely upon resolution of the sequencing products in denaturing polyacrylamide gels. This unit provides a detailed description of the setup, electrophoresis, and processing of such gels. In general,
Autor:
H, Ruan, K D, Lunnen, M E, Scott, L S, Moran, B E, Slatko, J J, Pelletier, E J, Hess, J, Benner, G G, Wilson, S Y, Xu
Publikováno v:
Moleculargeneral genetics : MGG. 252(6)
AvaI and BsoBI restriction endonucleases are isoschizomers which recognize the symmetric sequence 5'CYCGRG3' and cleave between the first C and second Y to generate a four-base 5' extension. The AvaI restriction endonuclease gene (avaIR) and methylas
Autor:
L E, Sears, L S, Moran, C, Kissinger, T, Creasey, H, Perry-O'Keefe, M, Roskey, E, Sutherland, B E, Slatko
Publikováno v:
BioTechniques. 13(4)
CircumVent thermal cycle and standard DNA sequencing protocols utilizing the cloned and highly thermostable VentR (exo-) DNA polymerase are described. The thermal cycle sequencing procedures are advantageous because they allow fast and simple semiaut