Zobrazeno 1 - 10
of 76
pro vyhledávání: '"B Degrève"'
Publikováno v:
BioTechniques, Vol 27, Iss 4, Pp 772-777 (1999)
We present a fast, convenient and inexpensive method that allows the automated, large-scale screening of chemical libraries for compounds that are converted by the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) into inhibitors of cell grow
Externí odkaz:
https://doaj.org/article/362dba905586471682c58b7cfb238123
Autor:
Kris Leslie, Alan Wright, Louise E. Bird, Jingshan Ren, B Degrève, Jan Balzarini, David K. Stammers
Publikováno v:
Journal of Biological Chemistry. 278:24680-24687
Herpes virus thymidine kinases are responsible for the activation of nucleoside antiviral drugs including (E)-5-(2-bromovinyl)-2'-deoxyuridine. Such viral thymidine kinases (tk), beside having a broader substrate specificity compared with host cell e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58bb17539d303dadb96c3cd33e47c39f
https://doi.org/10.1074/jbc.m302025200
https://doi.org/10.1074/jbc.m302025200
Autor:
Elisa Durini, Jan Balzarinia, Luca Porcu, Anna Karlsson, Stefano Manfredini, B Degrève, Chaoyong Zhu, Erik De Clercq
Publikováno v:
Biochemical Pharmacology. 61:727-732
Introduction of a bulky lipophilic acyl entity at the 2'-OH position of both 1-beta-D-arabinofuranosylthymine (araT) and (E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil (BVaraU), consistently resulted in a marked ( approximately 10-fold) increas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::410bf011588bd61cc04eadef948812d8
https://doi.org/10.1016/s0006-2952(01)00529-9
https://doi.org/10.1016/s0006-2952(01)00529-9
Publikováno v:
Molecular Pharmacology. 59:285-293
The broad substrate specificity of herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) has provided the basis for selective antiherpetic therapy and, more recently, suicide gene therapy for the treatment of cancer. We have now constructed an HS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2245a01832ed80da5cf55bc912c5923a
https://doi.org/10.1124/mol.59.2.285
https://doi.org/10.1124/mol.59.2.285
Publikováno v:
Gene Therapy. 7:1543-1552
We evaluated the molecular mechanism of resistance in herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene-transfected murine mammary carcinoma (FM3ATK-/HSV-1 TK+) cells, that were selected for resistance against (E)-5-(2-bromovinyl)-2'-deo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc797350f55152ff59f38e791e169748
https://doi.org/10.1038/sj.gt.3301278
https://doi.org/10.1038/sj.gt.3301278
Autor:
Jan Balzarini, Roger Huybrechts, Sigrid Hatse, Michael Breuer, Erik De Clercq, Magnus Johansson, B Degrève, Anna Karlsson
Publikováno v:
Europe PubMed Central
The antiherpetic agent (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) was found to be an efficient substrate for recombinant Drosophila melanogaster-deoxyribonucleoside kinase with a K(m) of 4.5 microM and a V(max) of 400 nmol/microg protein/h compared
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0194cb8945d8114a7910c8a3737d48a
https://doi.org/10.1124/mol.57.4.811
https://doi.org/10.1124/mol.57.4.811
Publikováno v:
Biochemical and Biophysical Research Communications. 264:338-342
We have reported previously that the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) fused with green fluorescent protein (GFP) is localized in the nucleus of HSV-1 TK-GFP gene-transfected cells (Degrève et al. (1998) J. Virol. 72, 9535-95
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19dcf5e34a56622b47e80f5699156252
https://doi.org/10.1006/bbrc.1999.1485
https://doi.org/10.1006/bbrc.1999.1485
Publikováno v:
Journal of Biological Chemistry. 274:23814-23819
A Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) was reported to phosphorylate all four natural deoxyribonucleosides as well as several nucleoside analogs (Munch-Petersen, B., Piskur, J., and Sondergaard, L. (1998) J. Biol. Chem. 273, 39
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9ec9654f2b1eccea4a844a64b4a4a27
https://doi.org/10.1074/jbc.274.34.23814
https://doi.org/10.1074/jbc.274.34.23814
Publikováno v:
Gene Therapy. 6:162-170
Introduction of the herpes simplex virus type 1 thymidine kinase gene into tumor cells, followed by the administration of the antiherpes nucleoside analogue ganciclovir has been demonstrated to be effective in eliminating solid tumors in animals. The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::276fb23fca4ebc2a358a7e0d98ae7100
https://doi.org/10.1038/sj.gt.3300806
https://doi.org/10.1038/sj.gt.3300806
Autor:
B Degrève, Michel Vandeputte, Sigrid Hatse, Jan Balzarini, Lieve Naesens, E. De Clercq, Constant Segers, Mark Waer
Publikováno v:
ResearcherID
The acyclic nucleoside phosphonate 9-(2-phosphonyl-methoxyethyl)adenine (PMEA) is a potent and selective antiretroviral agent which is currently evaluated in its oral prodrug form, bis(POM)PMEA (adefovir dipivoxil), in phase II and III clinical trial
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89471815fea66dbaa63c6b17d48aec02
https://doi.org/10.1002/(sici)1097-0215(19980518)76:4<595::aid-ijc24>3.0.co
https://doi.org/10.1002/(sici)1097-0215(19980518)76:4<595::aid-ijc24>3.0.co