Výsledky vyhledávání - "Börje Norling"

Culture-independent quantification of Salmonella enterica in carcass gauze swabs by flotation prior to real-time PCR

Autor: Jenny Schelin, Håkan Vigre, Börje Norling, Jeffrey Hoorfar, Charlotta Löfström, Peter Rådström

Publikováno v: International Journal of Food Microbiology. 145:S103-S109

To facilitate quantitative risk assessment in the meat production chain, there is a need for culture-independent quantification methods. The aim of this study was to evaluate the use of flotation, a non-destructive sample preparation method based on

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::670c19a1472f55056e3280e86918f475
https://doi.org/10.1016/j.ijfoodmicro.2010.03.042

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Quantification of Campylobacter spp. in Chicken Rinse Samples by Using Flotation prior to Real-Time PCR

Autor: Börje Norling, Mansel W. Griffiths, Petra F. G. Wolffs, Peter Rådström, Jeffrey Hoorfar

Publikováno v: Applied and Environmental Microbiology. 71:5759-5764

Real-time PCR is fast, sensitive, specific, and can deliver quantitative data; however, two disadvantages are that this technology is sensitive to inhibition by food and that it does not distinguish between DNA originating from viable, viable noncult

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81fd3475c2155cefd9e95af617628a93
https://doi.org/10.1128/aem.71.10.5759-5764.2005

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Enumeration of Salmonella enterica in naturally contaminated animal feed samples by flotation prior to real-time quantitative PCR

Autor: Jenny Schelin, Börje Norling, Håkan Vigre, Peter Rådström, Charlotta Löfström

Publikováno v: Schelin, J, Norling, B, Vigre, H, Rådström, P & Löfström, C 2012, ' Enumeration of Salmonella enterica in naturally contaminated animal feed samples by flotation prior to real-time quantitative PCR ' 23rd International ICFMH Symposium, FoodMicro 2012, Istanbul, Turkey, 03/09/2012-07/09/2012, .
Technical University of Denmark Orbit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::6cf857975aff41bf9e781dc42c2614d8
http://orbit.dtu.dk/en/publications/enumeration-of-salmonella-enterica-in-naturally-contaminated-animal-feed-samples-by-flotation-prior-to-realtime-quantitative-pcr(8f67843f-543e-410c-8f33-57a22f0e71c3).html

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Advanced sample preparation for the molecular quantification of Staphylococcus aureus in artificially and naturally contaminated milk

Autor: Martin Wagner, Ingeborg Hein, Iuliana Aprodu, Jenny Schelin, Börje Norling, Georg Walcher, Peter Rådström, Anca Ioana Nicolau

Publikováno v: International journal of food microbiology. 145

Sample treatment is an essential element when using real-time PCR for quantification of pathogens directly on food samples. This study comparatively evaluated three different principles of sample treatment, i.e. immunomagnetic separation based on pha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5bf28a328a5bdcb69ac04654e427d8d
https://pubmed.ncbi.nlm.nih.gov/21035221

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Flotation—A New Method to Circumvent PCR Inhibitors in the Diagnosis of Lawsonia intracellularis

Autor: Börje Norling, Anders Gunnarson, Magdalena Jacobson, Anna Aspán

Publikováno v: International Journal of Microbiology, Vol 2009 (2009)
International Journal of Microbiology

The obligate intracellular bacteriumLawsonia intracellulariscauses enteritis and poor growth in weaned pigs. Cultivation is difficult and diagnosisante mortemis mainly based on techniques such as polymerase chain reaction. However, false negative res

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::831b93a4770b2520d3b720a593a0d0e2
https://doaj.org/article/7f4a6e2b3c08416da5eb979cb34a8331

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Simultaneous quantification of pathogenic Campylobacter and Salmonella in chicken rinse fluid by a flotation and real-time multiplex PCR procedure

Autor: Petra F. G. Wolffs, Mansel W. Griffiths, Börje Norling, Kari Glencross

Publikováno v: International journal of food microbiology. 117(1)

A procedure for simultaneous quantification of Campylobacter and Salmonella spp. in poultry skin rinse fluids by a flotation and real-time multiplex PCR method is described. Flotation of the target organisms in a discontinuous density gradient separa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ab00cda1648415035c562cc150761fd
https://pubmed.ncbi.nlm.nih.gov/17462769

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Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells

Autor: Börje Norling, Petra F. G. Wolffs, Peter Rådström

Publikováno v: Journal of microbiological methods. 60(3)

Real-time PCR technology is increasingly used for detection and quantification of pathogens in food samples. A main disadvantage of nucleic acid detection is the inability to distinguish between signals originating from viable cells and DNA released

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e1bae9be009a3e09ad771829a5ea12a
https://pubmed.ncbi.nlm.nih.gov/15649533

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Rapid quantification of yersinia enterocolitica in pork samples by a novel sample preparation method, flotation, prior to real-time PCR

Autor: Börje Norling, Petra F. G. Wolffs, Rickard Knutsson, Peter Rådström

Publikováno v: Journal of Clinical Microbiology; 42(3), pp 1042-1047 (2004)

The development of real-time PCR thermal cycles in the late 1990s has opened up the possibility of accurate quantification of microorganisms in clinical, environmental, and food samples. However, a lack of suitable sample preparation methods that all

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b37dda21e064b1a7ddcfd64def436c80
http://lup.lub.lu.se/record/140490

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