Structural studies of bacterial biofilm matrix fibres

Autor: Böhning, J

Many bacteria live in multicellular communities called biofilms. Within the biofilm, cells are embedded in a self-secreted extracellular biofilm matrix, which provides numerous benefits to bacteria inside. Biofilms also form during human infection, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______1064::4a195792569ba93531f8208a85b73575
https://ora.ox.ac.uk/objects/uuid:925a5c6f-5740-49bd-959b-69294413bf36

Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar.

Autor: Esser TK; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK.; Thermo Fisher Scientific, 1 Boundary Park, Hemel Hempstead, Hertfordshire HP2 7GE, UK., Böhning J; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK., Önür A; Department of Chemistry, University of Konstanz, Konstanz 78457, Germany., Chinthapalli DK; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Eriksson L; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Grabarics M; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Fremdling P; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Konijnenberg A; Thermo Fisher Scientific, De Schakel 2, 5651GH Eindhoven, Netherlands., Makarov A; Thermo Fisher Scientific, Bremen 28199, Germany.; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, Netherlands., Botman A; Thermo Fisher Scientific, 5350 NE Dawson Creek Drive, Hillsboro, OR 97124, USA., Peter C; Department of Chemistry, University of Konstanz, Konstanz 78457, Germany., Benesch JLP; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Robinson CV; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK., Gault J; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK., Baker L; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK.; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK., Bharat TAM; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK., Rauschenbach S; Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.; Kavli Institute for NanoScience Discovery, Dorothy Crowfoot Hodgkin Building, Oxford OX1 3QU, UK.

Publikováno v: Science advances [Sci Adv] 2024 Feb 16; Vol. 10 (7), pp. eadl4628. Date of Electronic Publication: 2024 Feb 14.

Architecture of the biofilm-associated archaic Chaperone-Usher pilus CupE from Pseudomonas aeruginosa.

Autor: Böhning J; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Dobbelstein AW; Department of Protein Evolution, Max Planck Institute for Biology Tübingen, Tübingen, Germany., Sulkowski N; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Eilers K; Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United Kingdom., von Kügelgen A; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Tarafder AK; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom., Peak-Chew SY; Cell Biology Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, United Kingdom., Skehel M; Proteomics Science Technology Platform, The Francis Crick Institute, London, United Kingdom., Alva V; Department of Protein Evolution, Max Planck Institute for Biology Tübingen, Tübingen, Germany., Filloux A; Department of Life Sciences, MRC Centre for Molecular Bacteriology and Infection, Imperial College London, London, United Kingdom., Bharat TAM; Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, United Kingdom.

Publikováno v: PLoS pathogens [PLoS Pathog] 2023 Apr 14; Vol. 19 (4), pp. e1011177. Date of Electronic Publication: 2023 Apr 14 (Print Publication: 2023).

Compressed sensing for electron cryotomography and high-resolution subtomogram averaging of biological specimens

Autor: Böhning, J, Bharat, TAM, Collins, SM

Publikováno v: Structure. 30:408-417.e4

Cryoelectron tomography (cryo-ET) and subtomogram averaging (STA) allow direct visualization and structural studies of biological macromolecules in their native cellular environment, in situ. Often, low signal-to-noise ratios in tomograms, low partic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b55c2d8eee1041b5d3b73188ac63e6b1
https://doi.org/10.1016/j.str.2021.12.010

Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.

Autor: Esser TK; Department of Chemistry, University of Oxford, Oxford, OX1 3TF, UK. stephan.rauschenbach@chem.ox.ac.uk., Böhning J; Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK., Fremdling P; Department of Chemistry, University of Oxford, Oxford, OX1 3TF, UK. stephan.rauschenbach@chem.ox.ac.uk., Bharat T; Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, UK.; Structural Studies Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK., Gault J; Department of Chemistry, University of Oxford, Oxford, OX1 3TF, UK. stephan.rauschenbach@chem.ox.ac.uk., Rauschenbach S; Department of Chemistry, University of Oxford, Oxford, OX1 3TF, UK. stephan.rauschenbach@chem.ox.ac.uk.; Max Planck Institute for Solid State Research, Heisenbergstrasse 1, Stuttgart, DE-70569, Germany.

Publikováno v: Faraday discussions [Faraday Discuss] 2022 Nov 08; Vol. 240 (0), pp. 67-80. Date of Electronic Publication: 2022 Nov 08.