Best Minimally Modified Antisense Oligonucleotides According to Cell Nuclease Activity

Autor: Alain Laigle, Taraneh Djavanbakht Samani, Béatrice Jollès

Publikováno v: Antisense and Nucleic Acid Drug Development. 11:129-136

Minimally modified oligonucleotides belong to the second-generation antisense class. They are phosphodiester oligonucleotides with a minimum of phosphorothioate linkages in order to be protected against serum and cellular exonucleases and endonucleas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::077122b916d5788a7ab75cfdf4397bbe
https://doi.org/10.1089/108729001300338654

Targeting MDR1 gene: synthesis and cellular study of modified daunomycin-triplex-forming oligonucleotide conjugates able to inhibit gene expression in resistant cell lines

Autor: Vérène Stierlé, Maria Duca, Ludovic Halby, Catherine Senamaud-Beaufort, Massimo L. Capobianco, Paola B. Arimondo, Béatrice Jollès, Alain Laigle

Publikováno v: Molecular Pharmacology
Molecular Pharmacology, American Society for Pharmacology and Experimental Therapeutics, 2008, 73 (5), pp.1568-1577
Molecular pharmacology
73 (2008): 1568–1577. doi:10.1124/mol.107.042010
info:cnr-pdr/source/autori:Stierle, Verene (1); Duca, Maria (2); Halby, Ludovic (2); Senamaud-Beaufort, Catherine (2); Capobianco, Massimo L. (3); Laigle, Alain (1); Jolles, Beatrice (1); Arimondo, Paola B./titolo:Targeting MDR1 gene: Synthesis and cellular study of modified daunomycin-triplex-forming oligonucleotide conjugates able to inhibit gene expression in resistant cell lines/doi:10.1124%2Fmol.107.042010/rivista:Molecular pharmacology (Print)/anno:2008/pagina_da:1568/pagina_a:1577/intervallo_pagine:1568–1577/volume:73

Reversal of the multidrug-resistant (MDR) phenotype is very important for chemotherapy success. In fact, the expression of the MDR1 gene-encoded P-glycoprotein (P-gp) actively expels antitumor agents such as daunomycin (DNM) out of the cells, resulti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7afe2626028cdf85f24a7d412549b5c8
https://hal.archives-ouvertes.fr/hal-00449265

Minimally modified phosphodiester antisense oligodeoxyribonucleotide directed against the multidrug resistance gene mdr1

Autor: Imane Brigui, Alain Laigle, Taraneh Djavanbakht-Samani, Béatrice Jollès, Sophie Pigaglio

In the perspective of reversing multidrug resistance through antisense strategy while avoiding non-antisense effects of all-phosphorothioate oligonucleotides which non-specifically bind to proteins, a minimally modified antisense phosphodiester oligo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5a1951872dff44b3cc31e64fd3ead99
https://hal.science/hal-00109048

Opening of the extraordinarily stable mini-hairpin d(GCGAAGC)

Autor: Matthieu Réfrégiers, Béatrice Jollès, Alain Laigle

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, Oxford University Press, 1997, 25, pp.4608-4613. ⟨10.1093/nar/25.22.4608⟩
Scopus-Elsevier
Nucleic Acids Research, 1997, 25, pp.4608-4613. ⟨10.1093/nar/25.22.4608⟩

International audience; For the purposes of the antisense strategy oligodeoxyribonucleotides can be protected against serum and cell nuclease digestion by tagging at their 3′-end with a sequence naturally forming a very stable hairpin, d(GCGAAGC).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35251651684608d27d9d872505d4e9dd
https://hal.archives-ouvertes.fr/hal-01480366/document

Fluorescence Resonance Energy Transfer Analysis of the Degradation of an Oligonucleotide Protected by a Very Stable Hairpin

Autor: Béatrice Jollès, Alain Laigle, Matthieu Réfrégiers, Laurent Chinsky

Publikováno v: Journal of Biomolecular Structure and Dynamics
Journal of Biomolecular Structure and Dynamics, 1996, 14 (3), pp.365-371. ⟨10.1080/07391102.1996.10508131⟩
Scopus-Elsevier
Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1996, 14 (3), pp.365-371. ⟨10.1080/07391102.1996.10508131⟩

International audience; In vitro degradation of antisense oligonucleotides protected or not on their 3′ side against enzymatic attack by a naturally forming hairpin has been studied by fluorescence resonance energy transfer (FRET). The two oligonuc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b7ac47469debaf1d99cc3a9b1c546c4
https://hal.science/hal-01480966