Zobrazeno 1 - 10
of 479
pro vyhledávání: '"B, Witholt"'
Publikováno v:
Journal of Bacteriology. 176:6566-6571
We constructed a series of transposon vectors which allow efficient in vitro gene manipulation and subsequent introduction of cloned DNA into a variety of gram-negative bacteria. Transfer of the cloned fragment from these multicopy plasmids into self
Publikováno v:
Enzyme and Microbial Technology. 16:887-894
Escherichia coli recombinants expressing xylene oxygenase (XO) from the TOL plasmid pWWO of Pseudomonas putida mt-2 were used in two-liquid phase fermentations, with n-octane as a semi-inert second liquid phase, to produce epoxides derived from styre
Autor:
A. A. Verstegen‐Haaksma, Ben J.M. Jansen, H. J. Swarts, A. De Groot, N. Bottema‐Macgillavry, B. Witholt
Publikováno v:
ChemInform. 26
Publikováno v:
ACS Symposium Series ISBN: 9780841239173
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ef7e7e3beb3d5920aa10ca2aa9feaa38
https://doi.org/10.1021/bk-2005-0900.ch020
https://doi.org/10.1021/bk-2005-0900.ch020
Publikováno v:
Advances in biochemical engineering/biotechnology. 71
Poly(3-hydroxyalkanoates) (PHAs) constitute a large and versatile family of polyesters produced by various bacteria. PHAs are receiving considerable attention because of their potential as renewable and biodegradable plastics, and as a source of chir
A novel process for the purification of active medium-chain-length-polyhydroxyalkanoate (mcl-PHA) polymerase was developed. This process is based on solubilization and activation of inactive polymerase inclusion bodies by incubation with ion-exchange
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e927b7ce5a090bff9b5689b7d67cd632
https://europepmc.org/articles/PMC1221183/
https://europepmc.org/articles/PMC1221183/
Publikováno v:
Biotechnology and bioengineering. 69(1)
A whole cell biocatalytic process was developed to enable the efficient oxidation of styrene to chiral (S)-styrene oxide with an enantiomeric excess better than 99%. Recombinant Escherichia coli cells were employed to express the genes styAB encoding
Publikováno v:
European journal of biochemistry. 267(7)
We tested the synthesis and in vivo function of the inducible alkane hydroxylase of Pseudomonas oleovorans GPo1 in several Escherichia coli recombinants. The enzyme components (AlkB, AlkG and AlkT) were synthesized at various rates in different E. co
Publikováno v:
Biotechnology and bioengineering. 58(4)
E. coli HB101[pGEc47], which is able to convert octane to octanoate, but cannot oxidize octanoate further, was grown on defined medium with glucose as carbon source in batch and continuous culture. The biomass yield on glucose decreased from 0.32 +/-
Publikováno v:
Biotechnology and bioengineering. 58(1)
This paper shows that differences in growth behavior of Escherichia coli strain HB101 and strain HB101[pGEc47] can be related to yeast extract-enriched medium rather than plasmid properties. An optimal medium for growth of E. coli HB101[pGEc47] was d