Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution

Autor: Azul Zorzoli, Alasdair MacLean, Scott Nicholson, Alison Daniels, Stephen Hughes, Susan Bennet-Slater, Christine Tait-Burkard, Noha El Sakka, Rory Gunson, Kate Templeton

Publikováno v: BioTechniques, Vol 76, Iss 7, Pp 295-298 (2024)

In 2019, the European Union banned Triton X-100, a detergent widely used in laboratory diagnostics, including the Viral PCR Sample Solution (VPSS), and urged manufacturers to find environmentally sustainable alternatives. Tergitol 15-S-9 (VPSS2) has

Externí odkaz: https://doaj.org/article/894f8e032c58490c9961699e127ca8df

Evaluation of SARS-CoV-2 antibody point of care devices in the laboratory and clinical setting.

Autor: Kirsty McCance, Helen Wise, Jennifer Simpson, Becky Batchelor, Harriet Hale, Lindsay McDonald, Azul Zorzoli, Elizabeth Furrie, Charu Chopra, Frauke Muecksch, Theodora Hatziioannou, Paul D Bieniasz, Kate Templeton, Sara Jenks

Publikováno v: PLoS ONE, Vol 17, Iss 3, p e0266086 (2022)

SARS-CoV-2 antibody tests have been marketed to diagnose previous SARS-CoV-2 infection and as a test of immune status. There is a lack of evidence on the performance and clinical utility of these tests. We aimed to carry out an evaluation of 14 point

Externí odkaz: https://doaj.org/article/a92a9cd0d5a4495882ac0d916b2d7c74

Recombinant Group A Carbohydrate backbone embedded into Outer Membrane Vesicles is a potent vaccine candidate targeting Group A Streptococcus from Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis

Autor: Benjamin H. Meyer, Azul Zorzoli, Sarah Thomson, Mark Reglinski, Helge C. Dorfmueller, Sowmya Ajay Castro

BackgroundGroup A Streptococcus (GAS) are responsible for a wide range of human-exclusive infections, annually killing more than 500,000 people. Antibiotic resistance incidence of invasive GAS tripled in the past decade and emphasises the need to dev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::71448008507b87f2127431eac655bd3b
https://doi.org/10.1101/2021.11.12.468441

NDP-rhamnose biosynthesis and rhamnosyltransferases: building diverse glycoconjugates in nature

Autor: Ben A. Wagstaff, Helge C. Dorfmueller, Azul Zorzoli

Publikováno v: Wagstaff, B A, Zorzoli, A & Dorfmueller, H C 2021, ' NDP-rhamnose biosynthesis and rhamnosyltransferases: building diverse glycoconjugates in nature ', Biochemical Journal, vol. 478, no. 4, pp. 685-701 . https://doi.org/10.1042/BCJ20200505

Rhamnose is an important 6-deoxy sugar present in many natural products, glycoproteins, and structural polysaccharides. Whilst predominantly found as the l-enantiomer, instances of d-rhamnose are also found in nature, particularly in the Pseudomonads

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b02e09e78580cc5c9ec7370d0d592c38
https://doi.org/10.1042/BCJ20200505

Impact of serum procalcitonin on antibiotic stewardship in surgical high-dependency unit and intensive care unit settings

Autor: Laura Meney, Helge C. Dorfmueller, Benjamin Parcell, Aaron Taylor, Sharon Hilton-Christie, John Shone, Azul Zorzoli

Publikováno v: Access Microbiology. 2

Background Procalcitonin (PCT) testing is used as a biomarker for bacterial infection. We assessed the impact of using a PCT-guided algorithm in Ninewells hospital & Medical School (Dundee, Scotland, UK). Methods We conducted a retrospective analysis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7455d632f2de44287d94d95d85ea95e9
https://doi.org/10.1099/acmi.fis2019.po0191