A practice-changing culture method relying on shaking substantially increases mitochondrial energy metabolism and functionality of human liver cell lines.

Autor: Aziza A A Adam, Vincent A van der Mark, Joanne M Donkers, Manon E Wildenberg, Ronald P J Oude Elferink, Robert A F M Chamuleau, Ruurdtje Hoekstra

Publikováno v: PLoS ONE, Vol 13, Iss 4, p e0193664 (2018)

Practice-changing culturing techniques of hepatocytes are highly required to increase their differentiation. Previously, we found that human liver cell lines HepaRG and C3A acquire higher functionality and increased mitochondrial biogenesis when cult

Externí odkaz: https://doaj.org/article/dda135b9f4ac477fa76ca8c842d09a26

Assessment of the in vitro developmental toxicity of diethylstilbestrol and estradiol in the zebrafish embryotoxicity test

Autor: Aziza Hussein Bakheit Adam, Lenny Kamelia, Jochem Louisse, Ivonne M.C.M. Rietjens, Laura H.J. de Haan

Publikováno v: Toxicology in Vitro 72 (2021)
Toxicology in Vitro, 72

The present study investigated the developmental toxicity of diethylstilbestrol (DES) in the zebrafish embryotoxicity test (ZET). This was done to investigate whether the ZET would better capture the developmental toxicity of DES than the embryonic s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64c2ef32d2d0f5cf3892850c3e881082
https://pubmed.ncbi.nlm.nih.gov/33429043

Studying the role of estrogen receptor alpha in the developmental toxicity of diethylstilbestrol using alternative testing strategies

Autor: Aziza Hussein Bakheit Adam

Diethylstilbestrol (DES) is a synthetic estrogen that has been used between the 1940s and 1970s by pregnant women to prevent miscarriages and premature delivery by stimulating the synthesis of estrogen and progesterone in the placenta. However, use o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a942d32924105052b846f9de0f3c111
https://research.wur.nl/en/publications/studying-the-role-of-estrogen-receptor-alpha-in-the-developmental

HepaRG-Progenitor Cell Derived Hepatocytes Cultured in Bioartificial Livers Are Protected from Healthy- and Acute Liver Failure-Plasma Induced Toxicity

Autor: Ronald P.J. Oude Elferink, Thomas M. van Gulik, Vincent A. van der Mark, Philipp Treskes, Robert A. F. M. Chamuleau, Martien van Wenum, Aziza A. A. Adam, Aldo Jongejan, Ruurdtje Hoekstra, Perry D. Moerland

Publikováno v: Cellular Physiology and Biochemistry, Vol 48, Iss 5, Pp 2189-2204 (2018)
Cellular physiology and biochemistry, 48(5), 2189-2204. S. Karger AG

Background/Aims: For applicability of cell-based therapies aimed at the treatment of liver failure, such as bioartificial livers (BALs) and hepatocyte transplantation, it is essential that the applied hepatocytes tolerate exposure to the patient plas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d74ae72275fa9dbad0975e70e79bf0f9
https://doi.org/10.1159/000492560

The in vivo developmental toxicity of diethylstilbestrol (DES) in rat evaluated by an alternative testing strategy

Autor: Laura H.J. de Haan, Aziza Hussein Bakheit Adam, Ivonne M.C.M. Rietjens, Mengying Zhang, Jochem Louisse, Bennard van Ravenzwaay

Publikováno v: Archives of Toxicology 93 (2019) 7
Archives of Toxicology, 93(7), 2021-2033

In the present study, we evaluated an alternative testing strategy to quantitatively predict the in vivo developmental toxicity of the synthetic hormone diethylstilbestrol (DES). To this end, a physiologically based kinetic (PBK) model was defined th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5aa72f6caee1f2b650d882cd0f895689
https://research.wur.nl/en/publications/the-in-vivo-developmental-toxicity-of-diethylstilbestrol-des-in-r