Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Azadeh Sarfallah"'
Autor:
Gina Buchel, Ashok R. Nayak, Karl Herbine, Azadeh Sarfallah, Viktoriia O. Sokolova, Angelica Zamudio-Ochoa, Dmitry Temiakov
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Abstract DNA polymerase (DNAP) can correct errors in DNA during replication by proofreading, a process critical for cell viability. However, the mechanism by which an erroneously incorporated base translocates from the polymerase to the exonuclease s
Externí odkaz:
https://doaj.org/article/9e002b0d5b8c40d68114f7fa6af203f9
Publikováno v:
Proceedings of the National Academy of Sciences. 119
In live cells, phase separation is thought to organize macromolecules into membraneless structures known as biomolecular condensates. Here, we reconstituted transcription in condensates from purified mitochondrial components using optimized in vitro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4644de583190a28af31b4935d939769e
https://doi.org/10.1073/pnas.2207303119
https://doi.org/10.1073/pnas.2207303119
Autor:
Angelica Zamudio-Ochoa, Yaroslav I Morozov, Azadeh Sarfallah, Michael Anikin, Dmitry Temiakov
Publikováno v:
The FASEB Journal. 36
Recognition of mammalian mitochondrial promoters requires the concerted action of mitochondrial RNA polymerase (mtRNAP) and transcription initiation factors TFAM and TFB2M. In this work, we found that transcript slippage results in heterogeneity of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46a55e65147cfaa555afe27ba0f09c13
https://doi.org/10.1096/fasebj.2022.36.s1.l7814
https://doi.org/10.1096/fasebj.2022.36.s1.l7814
Phase separation organizes many membraneless structures in cells. The functional consequences of concentrating cellular machinery into biomolecular condensates, however, are largely unclear. One fundamental cellular function that has been linked to c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8bd6126dec6abcfb063c33f272e913a1
https://doi.org/10.1101/2021.12.30.474545
https://doi.org/10.1101/2021.12.30.474545
Publikováno v:
EMBO J
The intricate process of human mtDNA replication requires the coordinated action of both transcription and replication machineries. Transcription and replication events at the lagging strand of mtDNA prompt the formation of a stem-loop structure (Ori
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ccaf042458309a333a02775aa251a89
https://doi.org/10.15252/embj.2021107988
https://doi.org/10.15252/embj.2021107988
Autor:
Azadeh, Sarfallah, Dmitry, Temiakov
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2192
In vitro assay based on a reconstituted mitochondrial transcription system serves as a method of choice to probe the functional importance of proteins and their structural motifs. Here we describe protocols for transcription assays designed to probe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1598e6c087410c5775395ff9a49d680b
https://pubmed.ncbi.nlm.nih.gov/33230763
https://pubmed.ncbi.nlm.nih.gov/33230763
Autor:
Azadeh Sarfallah, Dmitry Temiakov
Publikováno v:
Methods in Molecular Biology ISBN: 9781071608333
In vitro assay based on a reconstituted mitochondrial transcription system serves as a method of choice to probe the functional importance of proteins and their structural motifs. Here we describe protocols for transcription assays designed to probe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d3bc116a1293b7ba54007b60dff8e193
https://doi.org/10.1007/978-1-0716-0834-0_3
https://doi.org/10.1007/978-1-0716-0834-0_3
Publikováno v:
Cell
Transcription in human mitochondria is driven by a single-subunit, factor-dependent RNA polymerase (mtRNAP). Despite of its critical role in both expression and replication of the mitochondrial genome, transcription initiation by mtRNAP remains poorl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4a0be0e652d117fab18cc2db8a8fb81