Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Axel Lezius"'
Autor:
Nils J. Færgeman, B. Rolf, Axel Lezius, Jens Knudsen, Friedrich Spener, Elke Oudenampsen-Krüger, Torsten Börchers
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1259:245-253
The coding part of the cDNA for bovine liver-type fatty acid binding protein (L-FABP) has been amplified by RT-PCR, cloned and used for the construction of an Escherichia coli (E. coli) expression system. The recombinant protein made up to 25% of the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e8281ad8e93947e76272cba397a031a
https://doi.org/10.1016/0005-2760(95)00170-0
https://doi.org/10.1016/0005-2760(95)00170-0
Autor:
Friedrich Spener, Bernfried Specht, Dirck Lassen, Heinz Rüterjans, Axel Lezius, Christian Lücke, Azita Mesgarzadeh, Marina Kveder, Jürgen M. Schmidt
Publikováno v:
European Journal of Biochemistry. 230:266-280
The three-dimensional structure of the hole form of recombinant cellular bovine heart fatty-acid-binding protein (H-FABP(c)), a polypeptide of 133 amino acid residues with a molecular mass of 15 kDa, has been determined by multidimensional homonuclea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1292b13a8213f0eb36f6861529f22f1f
https://doi.org/10.1111/j.1432-1033.1995.tb20560.x
https://doi.org/10.1111/j.1432-1033.1995.tb20560.x
Autor:
J. Muth, Finn Kirpekar, Eckhard Nordhoff, Franz Hillenkamp, Axel Lezius, Michael Karas, Stephanie Hahner, Chris Meier, Rainer Cramer, Joachim W. Engels
Publikováno v:
Journal of Mass Spectrometry. 30:99-112
UV and IR matrix-assisted laser desorption/ionization mass spectrometry (UV- and IR-MALDI) have demonstrated their potential for the accurate and sensitive mass determination of oligonucleotides. Metastable molecule ion fragmentation was found to be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7971fe4a2b8e9c1fadd3167605ddcc4b
https://doi.org/10.1002/jms.1190300116
https://doi.org/10.1002/jms.1190300116
Autor:
Friedrich Spener, Dirck Lassen, Christian Lücke, Arno Kromminga, Heinz Rüterjans, Axel Lezius
Publikováno v:
Molecular and Cellular Biochemistry. 123:15-22
Fatty acid-binding protein (FABP) from bovine heart, a 15 kDa cytoplasmic protein has been investigated by multi-dimensional homonuclear and heteronuclear NMR-spectroscopy. Perdeuterated palmitic acid has been used as fatty acid ligand. The tertiary
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91bd252cdc164f59bfdb30a05392a2a5
https://doi.org/10.1007/bf01076470
https://doi.org/10.1007/bf01076470
Autor:
Michael Karas, Stephanie Hahner, Karsten Kristiansen, Franz Hillenkamp, Axel Lezius, Eckhard Nordhoff, Peter Roepstorff, Finn Kirpekar
Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dae7d26febbaadfef0833809538a501d
https://europepmc.org/articles/PMC308382/
https://europepmc.org/articles/PMC308382/
Autor:
Axel Lezius, Peter Roepstorff, Michael Karas, Rainer Cramer, Stephanie Hahner, Eckhard Nordhoff, Karsten Kristiansen, Finn Kirpekar, Franz Hillenkamp
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfaf5e530554e61af6b58ff50ef8cd48
https://europepmc.org/articles/PMC308196/
https://europepmc.org/articles/PMC308196/
Autor:
Franz Hillenkamp, Friedrich Spener, Arnd Ingendoh, Elke Oudenampsen-Krüger, Bernfried Specht, Axel Lezius
Publikováno v:
Journal of biotechnology. 33(3)
An expression vector for bovine heart fatty acid-binding protein (H-FABP) was constructed by introducing the coding part of the cDNA into the pET-3d vector. Transformed Escherichia coli strain BL21 (DE3)pLysS produced functional recombinant H-FABP up
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cadc28c2f1d2b18ab6e3aa04d71ba61
https://pubmed.ncbi.nlm.nih.gov/7764738
https://pubmed.ncbi.nlm.nih.gov/7764738
Publikováno v:
European journal of biochemistry. 215(3)
In the course of our studies on structure/function relationships of fatty-acid-binding proteins, we reported earlier that the two isoforms of the 15-kDa cardiac fatty-acid-binding protein (cFABP) from bovine heart only differ in one position; Asn98 i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e87cd97867763478e6e7b28cc45cae6a
https://pubmed.ncbi.nlm.nih.gov/8354262
https://pubmed.ncbi.nlm.nih.gov/8354262
Publikováno v:
Molecular and cellular biochemistry. 98(1-2)
The coding part of the cDNA of cardiac fatty acid-binding protein (cFABP) from bovine heart was cloned into the vector pKK233-2. After induction with isopropyl-beta-D-thiogalactopyranoside cFABP was found in a soluble form in the cytosol of plasmid t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a9e4a02be2585b09ef0f24d76444763
https://pubmed.ncbi.nlm.nih.gov/2266972
https://pubmed.ncbi.nlm.nih.gov/2266972
Publikováno v:
Molecular and Cellular Biochemistry. 98
The unicellular eukaryotic microorganism, Saccharomyces cerevisiae, transformed with a plasmid containing a cDNA fragment encoding bovine heart fatty acid-binding protein (H-FABP) under the control of the inducible yeast GAL10 promoter, expressed FAB
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::270456d0a166b23a004efa71e308556c
https://doi.org/10.1007/bf00231369
https://doi.org/10.1007/bf00231369