Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Avigail Posner"'
Publikováno v:
BioTechniques, Vol 20, Iss 4, Pp 662-668 (1996)
Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protei
Externí odkaz:
https://doaj.org/article/0c4e45d916bc4c62a28dc9247317ef65
Autor:
Robert V. Talanian, Po-Wai Yuen, Daniel Stafford, Avigail Posner, Kevin K.W. Wang, Hamish Allen, Rathna Nath, Iradj Hajimohammadreza, Kadee J. Raser, Richard B. Gilbertsen
Publikováno v:
Biochemical Journal. 319:683-690
The cytoskeletal protein non-erythroid alpha-spectrin is well documented as an endogenous calpain substrate, especially under pathophysiological conditions. In cell necrosis (e.g. maitotoxin-treated neuroblastoma SH-SY5Y cells), alpha-spectrin breakd
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e908c233c5fde806f94cc0a8aa53161
https://doi.org/10.1042/bj3190683
https://doi.org/10.1042/bj3190683
Autor:
Charles Taylor, Kadee J. Raser, Avigail Posner, John J. Cordon, Kevin K.W. Wang, Nancy C. Kupina, Gerald P. Schielke, Mark G. Vartanian
Publikováno v:
Developmental Brain Research. 95:169-175
This study was performed to investigate whether the anticonvulsant phenytoin has neuroprotective effect in a model of hypoxia-ischemia with neonatal rats. The left carotid artery of each rat was ligated, followed by 3 h of hypoxic exposure (8% O2) in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59a1d84614dfd418b600448726953bea
https://doi.org/10.1016/0165-3806(96)00073-9
https://doi.org/10.1016/0165-3806(96)00073-9
Publikováno v:
Archives of biochemistry and biophysics. 319(1)
A zymographic assay for calpains in nondenaturing casein-containing polyacrylamide gels was developed. Calpain samples were run into the polyacrylamide gels by electrophoresis using a Tris-glycine buffer containing 1 mM EGTA to stabilize calpains. Up
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31abc6bc2bf5441956d1efca43a80b50
https://pubmed.ncbi.nlm.nih.gov/7771786
https://pubmed.ncbi.nlm.nih.gov/7771786