Výsledky vyhledávání

Comparison ofMycoplasma synoviaeisolates by immunoblotting

Autor: David H. Ley, Avakian Ap, Stanley H. Kleven

Publikováno v: Avian Pathology. 21:633-642

SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot profiles using antisera from M. synoviae F10-2AS-inoculated chickens detected heterogeneity among 10 M. synoviae isolates. This variation was primarily seen in immunoblots as a difference i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7200e50aaf199e95c30d60cbaf7d99ba
https://doi.org/10.1080/03079459208418884

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Comparison of Mycoplasma gallisepticum strains and identification of immunogenic integral membrane proteins with Triton X-114 by immunoblotting

Autor: David H. Ley, Avakian Ap, Stanley H. Kleven

Publikováno v: Veterinary microbiology. 29(3-4)

Pooled chicken antisera from 33 and 77 days post Mycoplasma gallisepticum strain R contact-exposure reacted with cell proteins of 19 M. gallisepticum strains. These pooled antisera reacted with more proteins and with greater intensity to reference st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a36a8390881ff5abd69ffd696c20e962
https://pubmed.ncbi.nlm.nih.gov/1771754

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Akademický článek

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Antibody Responses of Chickens to Inoculation with Mycoplasma gallisepticum Membrane Proteins in Immunostimulating Complexes

Autor: David H. Ley, Avakian Ap, McLaren Jm, Berkhoff Je

Publikováno v: Avian Diseases. 40:813

SUMMARY. Membrane proteins of Mycoplasma gallisepticum (MG) strain R were extracted with the detergent Mega-10 and incorporated into immunostimulating complexes (ISCOMs). A membrane protein of approximately 64 kD (p64) molecular weight was a major co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::60515a945b9873c45dc7f9c4a2abffc7
https://doi.org/10.2307/1592303

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Akademický článek

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Clinical Mycoplasma gallisepticum Infection in Multiplier Breeder and Meat Turkeys Caused by F Strain: Identification by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, Restriction Endonuclease Analysis, and the Polymerase Chain Reaction

Autor: Avakian Ap, Berkhoff Je, David H. Ley

Publikováno v: Avian Diseases. 37:854

In February 1991, a flock of North Carolina multiplier breeder turkeys experienced respiratory signs, sinusitis, airsacculitis, and increased mortality. Mycoplasma gallisepticum (MG) was isolated, and appropriate control measures were initiated. Ulti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1f891116107f83412aae056051e55941
https://doi.org/10.2307/1592041

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Inhibition of Mycoplasma gallisepticum Growth and Attachment to Chick Tracheal Rings by Antibodies to a 64-Kilodalton Membrane Protein of M. Gallisepticum

Autor: David H. Ley, Avakian Ap

Publikováno v: Avian Diseases. 37:706

SUMMARY. A Mycoplasma gallisepticum (MG) strain R protein of 64 kilodaltons (p64) was partially digested from the surface of the bacterium by trypsin. Monospecific polyclonal anti-p64 IgG inhibited attachment of MG to chick tracheal rings by as much

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3676875def8387073fcc34b5cf98e838
https://doi.org/10.2307/1592018

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Protective Immune Response to Mycoplasma gallisepticum Demonstrated in Respiratory-Tract Washings from M. gallisepticum-Infected Chickens

Autor: David H. Ley, Avakian Ap

Publikováno v: Avian Diseases. 37:697

SUMMARY. Chickens inoculated with Mycoplasma gallisepticum (MG) produced IgA, IgM, and IgG detectable in washings from the upper respiratory tract (URTW; nasal sinuses and turbinates) and lower respiratory tract (LRTW; trachea, lungs, and airsacs). U

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7db0ff27473e13c4118bf7eec692a8f5
https://doi.org/10.2307/1592017

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Breeder Turkey Hens Seropositive and Culture-Negative for Mycoplasma synoviae

Autor: Berkhoff Je, Ficken, David H. Ley, Avakian Ap

Publikováno v: Avian Diseases. 36:782

Four flocks of clinically normal turkey breeder hens were shown to have suspect and positive Mycoplasma synoviae (MS) hemagglutination-inhibition (HI), enzyme-linked immunosorbent assay, and, in some cases, serum plate agglutination serology in the a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ecb383738350f0fff4b3607ddcd3efa0
https://doi.org/10.2307/1591785

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Humoral Immune Response of Turkeys to Strain S6 and a Variant Mycoplasma gallisepticum Studied by Immunoblotting

Autor: Avakian Ap, Mary Ann T. McBride, David H. Ley

Publikováno v: Avian Diseases. 36:69

SUMMARY. Two putative variant Mycoplasma gallisepticum (MG) strains (M876 and M35), originally isolated from commercial turkeys, were compared with eight well-characterized MG strains by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::16c668740f4685f8cefbd5fc0608e1e2
https://doi.org/10.2307/1591718

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