Highly efficient baculovirus-mediated multigene delivery in primary cells

Autor: Maysam Mansouri, Itxaso Bellon-Echeverria, Aurélien Rizk, Zahra Ehsaei, Chiara Cianciolo Cosentino, Catarina S. Silva, Ye Xie, Frederick M. Boyce, M. Wayne Davis, Stephan C. F. Neuhauss, Verdon Taylor, Kurt Ballmer-Hofer, Imre Berger, Philipp Berger

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016)

Current viral gene delivery systems are limited in the amount of foreign DNA they can deliver to cells. Here the authors develop MultiPrime, a baculovirus-based vector system capable of multigene delivery into a wide variety of cells, and use Multipr

Externí odkaz: https://doaj.org/article/91e7d81de6ed4df8941fdab73f25c864

Competing G protein‐coupled receptor kinases balance G protein and β‐arrestin signaling

Autor: Domitille Heitzler, Guillaume Durand, Nathalie Gallay, Aurélien Rizk, Seungkirl Ahn, Jihee Kim, Jonathan D Violin, Laurence Dupuy, Christophe Gauthier, Vincent Piketty, Pascale Crépieux, Anne Poupon, Frédérique Clément, François Fages, Robert J Lefkowitz, Eric Reiter

Publikováno v: Molecular Systems Biology, Vol 8, Iss 1, Pp 1-17 (2012)

Abstract Seven‐transmembrane receptors (7TMRs) are involved in nearly all aspects of chemical communications and represent major drug targets. 7TMRs transmit their signals not only via heterotrimeric G proteins but also through β‐arrestins, whos

Externí odkaz: https://doaj.org/article/36341ec006974a4487849a5c5291ac17

The European Research Network on Signal Transduction (ERNEST) : Toward a Multidimensional Holistic Understanding of G Protein-Coupled Receptor Signaling

Autor: Jens Carlsson, Peter Kolb, David E. Gloriam, Andrew B. Tobin, Eddy Sotelo, Johanna K. S. Tiemann, Maria Waldhoer, Nina Vardjan, Mette M. Rosenkilde, Aurélien Rizk, Mickey Kosloff, Jana Selent, Gyorgy M. Keseru, Stefan Mordalski, Martha E. Sommer, Chris de Graaf

Publikováno v: Sommer, M E, Selent, J, Carlsson, J, De Graaf, C, Gloriam, D E, Keseru, G M, Kosloff, M, Mordalski, S, Rizk, A, Rosenkilde, M M, Sotelo, E, Tiemann, J K S, Tobin, A, Vardjan, N, Waldhoer, M & Kolb, P 2020, ' The European Research Network on Signal Transduction (ERNEST) : Toward a Multidimensional Holistic Understanding of G Protein-Coupled Receptor Signaling ', ACS Pharmacology & Translational Science, vol. 3, no. 2, pp. 361-370 . https://doi.org/10.1021/acsptsci.0c00024
ACS Pharmacol Transl Sci
ACS Pharmacology and Translational Science

G protein-coupled receptors (GPCRs) are intensively studied due to their therapeutic potential as drug targets. Members of this large family of transmembrane receptor proteins mediate signal transduction in diverse cell types and play key roles in hu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4374d747b2bc1fd8ae99f4fbb6b6fc8f
http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-433663

Arrestin-1 engineering facilitates complex stabilization with native rhodopsin

Autor: Xavier Deupi, Philipp Berger, Eshita Mutt, Aurélien Rizk, Martin K. Ostermaier, Jonas Mühle, Christian Peterhans, Jörg Standfuss, Florian Wilhelm, Raphael S. Haider, Gebhard F. X. Schertler

Publikováno v: Scientific Reports
Scientific Reports, 9
Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)

Arrestin-1 desensitizes the activated and phosphorylated photoreceptor rhodopsin by forming transient rhodopsin−arrestin-1 complexes that eventually decay to opsin, retinal and arrestin-1. Via a multi-dimensional screening setup, we identified and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa04867f8ce225e9a49171ec64be2f12
http://europepmc.org/articles/PMC6346018

Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh

Autor: Axel Niemann, Pietro Incardona, Maysam Mansouri, Ivo F. Sbalzarini, Aurélien Rizk, Gregory Paul, Philipp Berger, Milica Bugarski, Urs Ziegler

Publikováno v: Nature Protocols. 9:586-596

Detection and quantification of fluorescently labeled molecules in subcellular compartments is a key step in the analysis of many cell biological processes. Pixel-wise colocalization analyses, however, are not always suitable, because they do not pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a70775a221b53885c4742ec3af481ab1
https://doi.org/10.1038/nprot.2014.037