Tfam Knockdown Results in Reduction of mtDNA Copy Number, OXPHOS Deficiency and Abnormalities in Zebrafish Embryos

Autor: Auke B. C. Otten, Rick Kamps, Patrick Lindsey, Mike Gerards, Hélène Pendeville-Samain, Marc Muller, Florence H. J. van Tienen, Hubert J. M. Smeets

Publikováno v: Frontiers in Cell and Developmental Biology, Vol 8 (2020)

High mitochondrial DNA (mtDNA) copy numbers are essential for oogenesis and embryogenesis and correlate with fertility of oocytes and viability of embryos. To understand the pathology and mechanisms associated with low mtDNA copy numbers, we knocked

Externí odkaz: https://doaj.org/article/56a43e3547e342d7b74b4a094a8b8491

The noncoding RNA PRANCR regulates splicing of Fibronectin-1 to control keratinocyte proliferation and migration

Autor: Sun Bk, Amarbayar O, Auke B C Otten, Pengfei Cai, Kun Qu, Cheng B

Most human genes undergo alternative splicing (AS), but the regulation and functional consequences of most splicing events remain unknown. Long noncoding RNAs (lncRNAs) have recently been discovered to have novel roles in the regulation of AS. Here w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3b640eef1e1ac79b7b327efc5a0ad066
https://doi.org/10.1101/2021.06.22.449364

Tfam knockdown results in reduction of mtDNA copy number, OXPHOS deficiency and abnormalities in zebrafish embryos

Autor: Auke B. C. Otten, Rick Kamps, Patrick Lindsey, Mike Gerards, Hélène Pendeville-Samain, Marc Muller, Florence H. J. van Tienen, Hubert J. M. Smeets

Publikováno v: Frontiers in Cell and Developmental Biology, 8:381. Frontiers Media SA
Frontiers in Cell and Developmental Biology, Vol 8 (2020)
Frontiers in Cell and Developmental Biology

High mitochondrial DNA (mtDNA) copy numbers are essential for oogenesis and embryogenesis and correlate with fertility of oocytes and viability of embryos. To understand the pathology and mechanisms associated with low mtDNA copy numbers, we knocked

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe267eb04c13c942217ae31afe0c4a16

Differences in Strength and Timing of the mtDNA Bottleneck between Zebrafish Germline and Non-germline Cells

Autor: Bianca J.C. van den Bosch, Tom E. J. Theunissen, Erez Raz, Katsiaryna Tarbashevich, Ellen H Lambrichs, Iris B W Boesten, Marc Muller, Marie Winandy, Josien G. Derhaag, Auke B C Otten, Jo Vanoevelen, Aafke P.A. van Montfoort, H.J.M. Smeets, Mike Gerards

Publikováno v: Cell Reports, Vol 16, Iss 3, Pp 622-630 (2016)
Cell Reports, 16(3), 622-630. Cell Press

We studied the mtDNA bottleneck in zebrafish to elucidate size, timing, and variation in germline and non-germline cells. Mature zebrafish oocytes contain, on average, 19.0 × 10(6) mtDNA molecules with high variation between oocytes. During embryoge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec7246876139b6f67e55a63c5c835a0b
http://www.sciencedirect.com/science/article/pii/S221112471630763X

Mutation-specific effects in germline transmission of pathogenic mtDNA variants

Autor: Christine E. M. de Die-Smulders, David C. Samuels, Hubert J.M. Smeets, Mary Herbert, Patrick J. Lindsey, Auke B C Otten, Patrick F. Chinnery, Marion Drüsedau, Suzanne C E H Sallevelt, Phillippa J. Carling, Sabine Spierts, Joseph C F M Dreesen, Aimee D C Paulussen

Publikováno v: Human Reproduction, 33(7), 1331-1341. Oxford University Press

STUDY QUESTION: The primary objective was to assess whether germline selection events play a role during the inheritance of pathogenic mitochondrial DNA (mtDNA) variants and to provide possible underlying mechanisms for this selection. SUMMARY ANSWER

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::481d31383cef5d0e286207cc40994eab
https://pubmed.ncbi.nlm.nih.gov/29850888