Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin.

Autor: Winnie Tan, Vincent J Murphy, Aude Charron, Sylvie van Twest, Michael Sharp, Angelos Constantinou, Michael W Parker, Wayne Crismani, Rohan Bythell-Douglas, Andrew J Deans

Publikováno v: PLoS ONE, Vol 15, Iss 2, p e0229000 (2020)

Site-specific conjugation of ubiquitin onto a range of DNA repair proteins regulates their critical functions in the DNA damage response. Biochemical and structural characterization of these functions are limited by an absence of tools for the purifi

Externí odkaz: https://doaj.org/article/04c71120a0b94b45ae74b931dd2c2641

TAFA4 relieves injury-induced mechanical hypersensitivity through LDL receptors and modulation of spinal A-type K+ current

Autor: Catarina Santos, Rafaelle Rossignol, Ana Reynders, Jean-Yves Springael, Pascale Malapert, Irène Marics, Francis Castets, Marc Parmentier, Andrew J. Saurin, Aziz Moqrich, Sophie Ugolini, Nadine Clerc, Jean-Marc Goaillard, Sungjae Yoo, Abderazzak El Khallouqi, Aude Charron, Stéphane Gaillard

Publikováno v: Cell Reports
Cell Reports, 2021, 37 (4), pp.109884. ⟨10.1016/j.celrep.2021.109884⟩
Cell Reports, Elsevier Inc, 2021, 37 (4), pp.109884. ⟨10.1016/j.celrep.2021.109884⟩

Pain, whether acute or persistent, is a serious medical problem worldwide. However, its management remains unsatisfactory, and new analgesic molecules are required. We show here that TAFA4 reverses inflammatory, postoperative, and spared nerve injury

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78e722239cd59751b7510795b8b93fcf
https://doi.org/10.1016/j.celrep.2021.109884