Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Attilio Fabbretti"'
Autor:
Thuy Duong Pham, Chiara Poletti, Therese Manuela Nloh Tientcheu, Massimiliano Cuccioloni, Roberto Spurio, Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-16 (2024)
Abstract Cell-free protein synthesis (CFPS) systems offer a versatile platform for a wide range of applications. However, the traditional methods for detecting proteins synthesized in CFPS, such as radioactive labeling, fluorescent tagging, or electr
Externí odkaz:
https://doaj.org/article/7efb013c88e849d39b18fb47e9aa273f
Autor:
Ana Quiroz-Huanca, Ana Sanchez-Castro, Pablo Soriano-Castillo, Chiara Poletti, Therese Manuela Nloh Tientcheu, Attilio Fabbretti, Anna Maria Giuliodori, Pohl Milon
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103448- (2024)
Summary: Here, we present an in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of split-GFP for translation test (FAST), based on the small fragment GFP11 binding to GFP1-10fast. We detail the expression and purifi
Externí odkaz:
https://doaj.org/article/377b6e1fe4954eb4a617591af978f478
Autor:
Qi Cui, Han-Dong Yu, Qi-Jun Xu, Yue Liu, Yu-Ting Wang, Peng-Hui Li, Ling-Cong Kong, Hai-Peng Zhang, Xiu-Yun Jiang, Anna Maria Giuliodori, Attilio Fabbretti, Cheng-Guang He, Hong-Xia Ma
Publikováno v:
Frontiers in Microbiology, Vol 14 (2023)
IntroductionThe continued emergence and spread of multidrug-resistant (MDR) bacterial pathogens require a new strategy to improve the efficacy of existing antibiotics. Proline-rich antimicrobial peptides (PrAMPs) could also be used as antibacterial s
Externí odkaz:
https://doaj.org/article/cb705d28707544198b022d388fbd7317
Autor:
Rossana Petrilli, Attilio Fabbretti, Alex Cerretani, Kathleen Pucci, Graziella Pagliaretta, Matteo Picciolini, Valerio Napolioni, Maurizio Falconi
Publikováno v:
Microorganisms, Vol 11, Iss 2, p 311 (2023)
The increasing amounts of municipal solid waste and their management in landfills caused an increase in the production of leachate, a liquid formed by the percolation of rainwater through the waste. Leachate creates serious problems to municipal wast
Externí odkaz:
https://doaj.org/article/edeca488a6ef48a2bdfeb3dc6c6c19fe
Autor:
Lucia Cimarelli, Anna Maria Giuliodori, Anna Brandi, Karolina Adamkiewicz, Roberto Spurio, Attilio Fabbretti
Publikováno v:
BioDiscovery, Vol 20, Iss , Pp 1-5 (2017)
Externí odkaz:
https://doaj.org/article/f198ed3d73ef44feba79d640cef759c2
Characterization of the Self-Resistance Mechanism to Dityromycin in the Streptomyces Producer Strain
Autor:
Attilio Fabbretti, Retina Çapuni, Anna Maria Giuliodori, Lucia Cimarelli, Antonino Miano, Valerio Napolioni, Anna La Teana, Roberto Spurio
Publikováno v:
mSphere, Vol 4, Iss 5 (2019)
ABSTRACT Dityromycin is a peptide antibiotic isolated from the culture broth of the soil microorganism Streptomyces sp. strain AM-2504. Recent structural studies have shown that dityromycin targets the ribosomal protein S12 in the 30S ribosomal subun
Externí odkaz:
https://doaj.org/article/992cef27e8be444d9b270146ff23ec31
Publikováno v:
Microorganisms, Vol 8, Iss 10, p 1558 (2020)
Second messenger nucleotides, such as guanosine penta- or tetra-phosphate, commonly referred to as (p)ppGpp, are powerful signaling molecules, used by all bacteria to fine-tune cellular metabolism in response to nutrient availability. Indeed, under n
Externí odkaz:
https://doaj.org/article/670ecc7db56a4d76b4452f4d15cb9e18
Autor:
Anna Maria Giuliodori, Anna Brandi, Shivaram Kotla, Francesco Perrozzi, Roberto Gunnella, Luca Ottaviano, Roberto Spurio, Attilio Fabbretti
Publikováno v:
PLoS ONE, Vol 12, Iss 8, p e0183952 (2017)
Graphene oxide (GO) is a promising material for the development of cost-effective detection systems. In this work, we have devised a simple and rapid GO-based method for the sequence-specific identification of DNA molecules generated by PCR amplifica
Externí odkaz:
https://doaj.org/article/540fe5c4eefb4e07b2e66013d44ff1b1
Autor:
David Bulkley, Letizia Brandi, Yury S. Polikanov, Attilio Fabbretti, Michael O’Connor, Claudio O. Gualerzi, Thomas A. Steitz
Publikováno v:
Cell Reports, Vol 6, Iss 2, Pp 357-365 (2014)
The translocation of mRNA and tRNA through the ribosome is catalyzed by elongation factor G (EF-G), a universally conserved guanosine triphosphate hydrolase (GTPase). The mechanism by which the closely related decapeptide antibiotics dityromycin and
Externí odkaz:
https://doaj.org/article/0eef0a3e96dd4781ba82132d2fc7a839
Publikováno v:
Antibiotics, Vol 5, Iss 4, p 38 (2016)
Aminoglycoside antibiotics are widely used to treat infectious diseases. Among them, streptomycin and kanamycin (and derivatives) are of importance to battle multidrug-resistant (MDR) Mycobacterium tuberculosis. Both drugs bind the small ribosomal su
Externí odkaz:
https://doaj.org/article/cccca3f99e97483cad9aa876f5a60adb