Automatic deep learning-driven label-free image-guided patch clamp system

Autor: Krisztian Koos, Gáspár Oláh, Tamas Balassa, Norbert Mihut, Márton Rózsa, Attila Ozsvár, Ervin Tasnadi, Pál Barzó, Nóra Faragó, László Puskás, Gábor Molnár, József Molnár, Gábor Tamás, Peter Horvath

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)

Patch clamp recording of neurons is slow and labor-intensive. Here the authors present a method for automated deep learning driven label-free image guided patch clamp physiology to perform measurements on hundreds of human and rodent neurons.

Externí odkaz: https://doaj.org/article/752426adf88042c3b1fc84b42821906d

Human neocortical expansion involves glutamatergic neuron diversification

Autor: Tim S. Heistek, Thomas Braun, Natalia A. Goriounova, Michael Tieu, Lindsay Ng, Michael Hawrylycz, Kris Bickley, Anton Arkhipov, Colin Farrell, Trangthanh Pham, Alexandra Glandon, Daniel Park, Gábor Molnár, Herman Tung, Allan R. Jones, Lisa Keene, Gáspár Oláh, Thomas Chartrand, Amy Torkelson, Jae Geun Yoon, Rachel A. Dalley, Aaron Szafer, Nick Dee, Brian E. Kalmbach, Eliza Barkan, Allison Beller, Krissy Brouner, Andrew L. Ko, Alex M. Henry, Viktor Szemenyei, Julie Nyhus, Staci A. Sorensen, Samuel Dingman Lee, Norbert Mihut, Amy Bernard, Lisa Kim, Anatoly Buchin, Melissa Gorham, Lucas T. Graybuck, Lydia Potekhina, Katelyn Ward, Caitlin S. Latimer, Aaron Oldre, Gabe J. Murphy, Boaz P. Levi, Trygve E. Bakken, René Wilbers, Jonathan T. Ting, Kimberly A. Smith, Amanda Gary, Songlin Ding, Alice Mukora, Matthew Kroll, Anoop P. Patel, Wayne Wakeman, Hongkui Zeng, Nadezhda Dotson, Rusty Mann, Victoria Omstead, Leona Mezei, Desiree A. Marshall, Shea Ransford, Lydia Ng, Sara Kebede, Gábor Tamás, Jeffrey G. Ojemann, Stephanie Mok, Nathan Hansen, Christina A. Pom, Brian Lee, Jim Berg, Ramkumar Rajanbabu, John W. Phillips, Philip R. Nicovich, Matthew Mallory, Richard G. Ellenbogen, Rachel Enstrom, Luke Esposito, Tim Jarsky, Di Jon Hill, Idan Segev, Darren Bertagnolli, Agata Budzillo, Sander Idema, Daniel L. Silbergeld, Costas A. Anastassiou, Chris Hill, Michelle Maxwell, Mean Hwan Kim, Charles Cobbs, Delissa McMillen, Bosiljka Tasic, Olivia Fong, Medea McGraw, Hong Gu, Kirsten Crichton, David Reid, Kristen Hadley, Lauren Alfiler, Manuel Ferreira, Elliot R. Thomsen, Kiet Ngo, Josef Sulc, Augustin Ruiz, Katherine Baker, Zizhen Yao, Erica J. Melief, Femke Waleboer, Hanchuan Peng, Grace Williams, Rebecca D. Hodge, Kyla Berry, Katherine E. Link, David Sandman, Tsega Desta, Christine Rimorin, Jeff Goldy, Ryder P. Gwinn, Djai B. Heyer, Changkyu Lee, Jeremy A. Miller, Nathan W. Gouwens, Pál Barzó, Attila Ozsvár, Huibert D. Mansvelder, Sergey L. Gratiy, Rafael Yuste, David Feng, Jessica Trinh, Clare Gamlin, Tamara Casper, C. Dirk Keene, Susan M. Sunkin, Tom Egdorf, Philip C. De Witt Hamer, Rebecca de Frates, Peter Chong, Szabina Furdan, Patrick R. Hof, Jasmine Bomben, Christiaan P. J. de Kock, Eline J. Mertens, Ed S. Lein, Anna A. Galakhova, Florence D’Orazi, Christof Koch, Madie Hupp

Publikováno v: Berg, J, Sorensen, S A, Ting, J T, Miller, J A, Chartrand, T, Buchin, A, Bakken, T E, Budzillo, A, Dee, N, Ding, S L, Gouwens, N W, Hodge, R D, Kalmbach, B, Lee, C, Lee, B R, Alfiler, L, Baker, K, Barkan, E, Beller, A, Berry, K, Bertagnolli, D, Bickley, K, Bomben, J, Braun, T, Brouner, K, Casper, T, Chong, P, Crichton, K, Dalley, R, de Frates, R, Desta, T, Lee, S D, D’Orazi, F, Dotson, N, Egdorf, T, Enstrom, R, Farrell, C, Feng, D, Fong, O, Furdan, S, Galakhova, A A, Gamlin, C, Gary, A, Glandon, A, Goldy, J, Gorham, M, Goriounova, N A, Gratiy, S, Graybuck, L, Gu, H, Hadley, K, Hansen, N, Heistek, T S, Henry, A M, Heyer, D B, Hill, D J, Hill, C, Hupp, M, Jarsky, T, Kebede, S, Keene, L, Kim, L, Kim, M H, Kroll, M, Latimer, C, Levi, B P, Link, K E, Mallory, M, Mann, R, Marshall, D, Maxwell, M, McGraw, M, McMillen, D, Melief, E, Mertens, E J, Mezei, L, Mihut, N, Mok, S, Molnar, G, Mukora, A, Ng, L, Ngo, K, Nicovich, P R, Nyhus, J, Olah, G, Oldre, A, Omstead, V, Ozsvar, A, Park, D, Peng, H, Pham, T, Pom, C A, Potekhina, L, Rajanbabu, R, Ransford, S, Reid, D, Rimorin, C, Ruiz, A, Sandman, D, Sulc, J, Sunkin, S M, Szafer, A, Szemenyei, V, Thomsen, E R, Tieu, M, Torkelson, A, Trinh, J, Tung, H, Wakeman, W, Waleboer, F, Ward, K, Wilbers, R, Williams, G, Yao, Z, Yoon, J G, Anastassiou, C, Arkhipov, A, Barzo, P, Bernard, A, Cobbs, C, de Witt Hamer, P C, Ellenbogen, R G, Esposito, L, Ferreira, M, Gwinn, R P, Hawrylycz, M J, Hof, P R, Idema, S, Jones, A R, Keene, C D, Ko, A L, Murphy, G J, Ng, L, Ojemann, J G, Patel, A P, Phillips, J W, Silbergeld, D L, Smith, K, Tasic, B, Yuste, R, Segev, I, de Kock, C P J, Mansvelder, H D, Tamas, G, Zeng, H, Koch, C & Lein, E S 2021, ' Human neocortical expansion involves glutamatergic neuron diversification ', Nature, vol. 598, no. 7879, pp. 151-158 . https://doi.org/10.1038/s41586-021-03813-8
Nature, 598(7879), 151-158. Nature Publishing Group
Nature
Nature, Vol. 598, No. 7879
Berg, J, Sorensen, S A, Ting, J T, Miller, J A, Chartrand, T, Buchin, A, Bakken, T E, Budzillo, A, Dee, N, Ding, S-L, Gouwens, N W, Hodge, R D, Kalmbach, B, Lee, C, Lee, B R, Alfiler, L, Baker, K, Barkan, E, Beller, A, Berry, K, Bertagnolli, D, Bickley, K, Bomben, J, Braun, T, Brouner, K, Casper, T, Chong, P, Crichton, K, Dalley, R, de Frates, R, Desta, T, Lee, S D, D’Orazi, F, Dotson, N, Egdorf, T, Enstrom, R, Farrell, C, Feng, D, Fong, O, Furdan, S, Galakhova, A A, Gamlin, C, Gary, A, Glandon, A, Goldy, J, Gorham, M, Goriounova, N A, Gratiy, S, Graybuck, L, Gu, H, Hadley, K, Hansen, N, Heistek, T S, Henry, A M, Heyer, D B, Hill, D, Hill, C, Hupp, M, Jarsky, T, Kebede, S, Keene, L, Kim, L, Kim, M-H, Kroll, M, Latimer, C, Levi, B P, Link, K E, Mallory, M, Mann, R, Marshall, D, Maxwell, M, McGraw, M, McMillen, D, Melief, E, Mertens, E J, Mezei, L, Mihut, N, Mok, S, Molnar, G, Mukora, A, Ng, L, Ngo, K, Nicovich, P R, Nyhus, J, Olah, G, Oldre, A, Omstead, V, Ozsvar, A, Park, D, Peng, H, Pham, T, Pom, C A, Potekhina, L, Rajanbabu, R, Ransford, S, Reid, D, Rimorin, C, Ruiz, A, Sandman, D, Sulc, J, Sunkin, S M, Szafer, A, Szemenyei, V, Thomsen, E R, Tieu, M, Torkelson, A, Trinh, J, Tung, H, Wakeman, W, Waleboer, F, Ward, K, Wilbers, R, Williams, G, Yao, Z, Yoon, J-G, Anastassiou, C, Arkhipov, A, Barzo, P, Bernard, A, Cobbs, C, de Witt Hamer, P C, Ellenbogen, R G, Esposito, L, Ferreira, M, Gwinn, R P, Hawrylycz, M J, Hof, P R, Idema, S, Jones, A R, Keene, C D, Ko, A L, Murphy, G J, Ng, L, Ojemann, J G, Patel, A P, Phillips, J W, Silbergeld, D L, Smith, K, Tasic, B, Yuste, R, Segev, I, de Kock, C P J, Mansvelder, H D, Tamas, G, Zeng, H, Koch, C & Lein, E S 2021, ' Human neocortical expansion involves glutamatergic neuron diversification ', Nature, vol. 598, no. 7879, pp. 151-158 . https://doi.org/10.1038/s41586-021-03813-8

The neocortex is disproportionately expanded in human compared with mouse1,2, both in its total volume relative to subcortical structures and in the proportion occupied by supragranular layers composed of neurons that selectively make connections wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c41f5015a297892f279cb761e2d9f21
https://hdl.handle.net/1871.1/df908a46-b182-41b3-b732-326dddbab23b

Predominantly linear summation of metabotropic postsynaptic potentials follows coactivation of neurogliaform interneurons

Autor: Gergely Komlósi, Attila Ozsvár, Judith Baka, Gábor Molnár, Gáspár Oláh, Gábor Tamás

Publikováno v: eLife
eLife, Vol 10 (2021)

Summation of ionotropic receptor-mediated responses is critical in neuronal computation by shaping input-output characteristics of neurons. However, arithmetics of summation for metabotropic signals are not known. We characterized the combined ionotr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::accc8ced9d13898f60b48cb3d0c31ec0
https://doi.org/10.7554/elife.65634

Automatic deep learning-driven label-free image-guided patch clamp system

Autor: Gábor Tamás, Norbert Mihut, Ervin Tasnadi, Gábor Molnár, Pál Barzó, Jozsef Molnar, László G. Puskás, Gáspár Oláh, Tamas Balassa, Attila Ozsvár, Krisztian Koos, Márton Rózsa, Peter Horvath, Nóra Faragó

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
Nature Communications

Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. Here, we demonstrate a tool that fully automatically performs electrophysiological recordings in label-free tissue slices. The automation covers the detection of cell

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81c537bcd8556bb0ec3079ea0218ca4b
http://hdl.handle.net/10138/329108

Mielinhüvely degradációt követő lipid törmelék eloszlás vizsgálata a központi idegrendszerben CARS mikroszkópiával és Agykérgi neurogliaform sejthálózat által kiváltott ionotróp és metabotróp gátló posztszinaptikus válaszok eltérő összegződése

Autor: Attila Ozsvár

Doktori disszertációmban kettő egymástól független kutatási témával foglalkozok: 1. Az axonokat körül ölelő mielinhüvelyt érintő degenerációs elváltozások ismertek a sclerosis multiplex (SM), az egyik leggyakoribb idegrendszeri me

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3f03acf845b7326582a1c80b7db3334f
https://doi.org/10.14232/phd.10270