Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Atsushi Ichiyanagi"'
Publikováno v:
AMB Express, Vol 13, Iss 1, Pp 1-9 (2023)
Abstract Enzymatic detection of citrulline, a potential biomarker for various diseases, is beneficial. However, determining citrulline levels requires expensive instrumental analyses and complicated colorimetric assays. Although l-amino acid oxidase/
Externí odkaz:
https://doaj.org/article/0f9f9b824eb84988923ee1fa75153b98
Publikováno v:
Journal of Biological Chemistry. 282:36652-36658
DyP, a unique dye-decolorizing enzyme from the fungus Thanatephorus cucumeris Dec 1, has been classified as a peroxidase but lacks homology to almost all other known plant peroxidases. The primary structure of DyP shows moderate sequence homology to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a8c951e6a05e2f0a6aa5c82d76f783c0
https://doi.org/10.1074/jbc.m706996200
https://doi.org/10.1074/jbc.m706996200
Autor:
Keiko Gomi, Hiroaki Kato, Kozo Hirokawa, Toru Nakatsu, Atsushi Ichiyanagi, Bunta Watanabe, Naoki Kajiyama
Publikováno v:
Bioorganicmedicinal chemistry letters. 25(18)
Fructosyl peptide oxidases (FPOXs) play a crucial role in the diagnosis of diabetes. Their main function is to cleave fructosyl amino acids or fructosyl peptides into glucosone and the corresponding amino acids/dipeptides. In this study, the substrat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::313eb37941523c399399284d3343335c
https://pubmed.ncbi.nlm.nih.gov/26235953
https://pubmed.ncbi.nlm.nih.gov/26235953
Autor:
Keiko Gomi, Atsushi Ichiyanagi, Yasuko Araki, Taro Yoshimura, Keiichi Ichikawa, Kozo Hirokawa, Ryoko Satake
Publikováno v:
Journal of bioscience and bioengineering. 120(5)
Glucose dehydrogenase (GDH) is of interest for its potential applications in the field of glucose sensors. To improve the performance of glucose sensors, GDH is required to have strict substrate specificity. A novel flavin adenine dinucleotide (FAD)-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75cd39a90fd9bf50cf64ab37b7e850d3
https://pubmed.ncbi.nlm.nih.gov/25912449
https://pubmed.ncbi.nlm.nih.gov/25912449
Publikováno v:
Acta crystallographica. Section F, Structural biology and crystallization communications. 69(Pt 2)
Fructosyl peptide oxidase (FPOX) catalyses the oxidation of α-glycated dipeptides such as N(α)-(1-deoxy-D-fructos-1-yl)-L-valyl-L-histidine (Fru-ValHis) and is used in the diagnosis of diabetes mellitus. Here, two thermostable mutants of FPOX, CFP-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::912352dad9bfa96591eb31b6f0d8035e
https://pubmed.ncbi.nlm.nih.gov/23385752
https://pubmed.ncbi.nlm.nih.gov/23385752
Publikováno v:
Biotechnology letters. 34(2)
Alkaline phosphatase catalyzes the hydrolysis of phosphomonoesters and is widely used in molecular biology techniques and clinical diagnostics. We expressed a recombinant alkaline phosphatase of the marine bacterium, Cobetia marina, in Escherichia co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fd54891224d45c0bafd80add24843f2
https://pubmed.ncbi.nlm.nih.gov/22009571
https://pubmed.ncbi.nlm.nih.gov/22009571
Publikováno v:
Applied microbiology and biotechnology. 78(5)
Fructosyl peptide oxidases are valuable for the determination of glycoproteins such as hemoglobin A1c. For practical use in clinical diagnosis, we applied directed evolution to improve the thermostability of these enzymes. After two rounds of random
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7baf518182b97f8831babe1a005b7dcc
https://pubmed.ncbi.nlm.nih.gov/18246344
https://pubmed.ncbi.nlm.nih.gov/18246344
The growth of suitably sized protein crystals is essential for protein structure determination by X-ray crystallography. In general, crystals are grown using a trial-and-error method. However, these methods have been modified with the advent of micro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0eb99f1560c38095e10721ca1b75a97b
https://europepmc.org/articles/PMC1952364/
https://europepmc.org/articles/PMC1952364/
