Zobrazeno 1 - 10
of 88
pro vyhledávání: '"Atsuo Nakata"'
Publikováno v:
Nucleic Acids Research. 28:1206-1210
A global mechanism of catabolite repression of the genus Bacillus comprises negative regulation exerted through the binding of the CcpA protein to the catabolite-responsive elements (cres) of the target genes. We searched for cre sequences in the Bac
Publikováno v:
Genes to Cells. 3(No. 4):209-220
BACKGROUND: All the ruvA, ruvB and ruvC mutants of Escherichia coli are sensitive to treatments that damage DNA, and are mildly defective in homologous recombination. It has been reported that the ruv mutants form nonseptate, multinuclear filaments a
Autor:
Kozo Makino, Hideo Shinagawa, Masashi Suzuki, Sigenobu Kimura, M Amemura, Atsuo Nakata, Takeshi Kawamoto
Publikováno v:
Journal of Molecular Biology. 259:15-26
We have identified the DNA-binding domain (DBD) of an Escherichia coli activator protein PhoB as its C-terminal 91 residues. Four amino acid positions in the PhoB DBD are found important for interaction with the RNA polymerase holoenzyme that contain
Autor:
Shigeharu Ueda, Hajime Fujio, Xiaoui Wang, Hideo Shinagawa, Jiro Seki, Kiyoshi Kondo, Xiaoliang Liu, Atsushi Saitoh, Nobuo Sakato, Shin-ichi Miyake, Akemi Ota, Takashi Kurimura, Atsuo Nakata, Michiko Watanabe, Yu Zhao
Publikováno v:
Microbiology and Immunology. 39:775-785
We investigated the murine antibody response to recombinant p17 (rp17) of human immunodeficiency virus type 1 (HIV-1) and the human antibody response directed to p17 in HIV-1 infection. Three large peptides covering residues 12-29, 53-87 and 87-115 o
Publikováno v:
Genes & Genetic Systems. 70:79-92
Three genomic DNA fragments containing the tyrosinase-encoding gene (TYR) of the Japanese pond frog, Rana nigromaculata, were cloned. The first, clone I, was isolated from a genomic library of sperm DNA using the mouse TYR cDNA as the probe and conta
Autor:
Kazuya Hashinaka, Eiji Ishikawa, Kaoru Shimada, Shinichi Oka, Seiichi Hashida, Atsuo Nakata, Hideo Shinagawa, Atsushi Saitoh
Publikováno v:
Journal of Immunological Methods. 172:179-187
Recombinant reverse transcriptase (RT) of HIV-1 was conjugated to β- d -galactosidase from Escherichia coli in three different ways. Maleimide groups were introduced into β- d -galactosidase molecules using N,N′-o-phenylenedimaleimide in the abse
Publikováno v:
Journal of Bacteriology. 175:1316-1324
By making operon fusions with lambda placMu53, we identified, cloned, and analyzed the phoH gene belonging to the phosphate (pho) regulon. We mapped the phoH gene at 23.6 min in the Escherichia coli genomic library (Y. Kohara, K. Akiyama, and K. Ison
Publikováno v:
Molecular and General Genetics MGG. 237:395-399
Escherichia coli RuvA and RuvB proteins are encoded by an SOS-regulated operon, which is involved in DNA repair and recombination. RuvB has weak ATPase activity, which is enhanced by the addition of RuvA and DNA, and RuvA and RuvB in the presence of
Autor:
Seiichi Hashida, Hideo Shinagawa, Atsuo Nakata, Kouichi Hirota, Shinichi Oka, Shuzo Matsushita, Atsushi Saitoh, Junichi Mimaya, Kaoru Shimada, Kazuya Hashinaka, Eiji Ishikawa
Publikováno v:
Journal of Clinical Laboratory Analysis. 7:353-364
For diagnosis of HIV-1 infection, attempts were made to detect anti-HIV-1 IgG in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant reverse transcriptase (RT) and p17 as antigens. Anti-HIV-1 IgG in ur
Publikováno v:
Genes & Development. 7:149-160
Transcription of the genes belonging to the phosphate (pho) regulon in Escherichia coli, which are induced by phosphate starvation, requires the specific activator protein PhoB in addition to the RNA polymerase holoenzyme containing the major sigma-f