Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Astrid D. Haase"'
Autor:
Parthena Konstantinidou, Zuzana Loubalova, Franziska Ahrend, Aleksandr Friman, Miguel Vasconcelos Almeida, Axel Poulet, Filip Horvat, Yuejun Wang, Wolfgang Losert, Hernan Lorenzi, Petr Svoboda, Eric A. Miska, Josien C. van Wolfswinkel, Astrid D. Haase
Publikováno v:
Cell Reports, Vol 43, Iss 10, Pp 114777- (2024)
Summary: PIWI-interacting RNAs (piRNAs) play a crucial role in safeguarding genome integrity by silencing mobile genetic elements. From flies to humans, piRNAs originate from long single-stranded precursors encoded by genomic piRNA clusters. How piRN
Externí odkaz:
https://doaj.org/article/d5ee216aeb984bc49d474c711d1860ea
Publikováno v:
Mobile DNA, Vol 14, Iss 1, Pp 1-13 (2023)
Abstract PIWI-interacting RNAs (piRNAs) are responsible for preventing the movement of transposable elements in germ cells and protect the integrity of germline genomes. In this review, we examine the common elements of piRNA-guided silencing as well
Externí odkaz:
https://doaj.org/article/d3a8e7c26a3d4956bc68bc19ea34b67e
Autor:
Astrid D. Haase
Publikováno v:
RNA Biology, Vol 19, Iss 1, Pp 1094-1102 (2022)
PIWI proteins and their associated PIWI-interacting RNAs (piRNAs) constitute a small RNA-based adaptive immune system that restricts the deleterious activity of mobile genetic elements to protect genome integrity. Self/nonself discrimination is at th
Externí odkaz:
https://doaj.org/article/960d41110cb7402ba7ff007f6d6bd620
Publikováno v:
iScience, Vol 25, Iss 6, Pp 104427- (2022)
Summary: PIWI-interacting RNAs (piRNAs) guard germline genomes against the deleterious action of mobile genetic elements. PiRNAs use extensive base-pairing to recognize their targets and variable 3′ends could change the specificity and efficacy of
Externí odkaz:
https://doaj.org/article/eaa70149f3de4390b34ae61fbb470c0e
Autor:
Chad B. Stein, Pavol Genzor, Sanga Mitra, Alexandra R. Elchert, Jonathan J. Ipsaro, Leif Benner, Sushil Sobti, Yijun Su, Molly Hammell, Leemor Joshua-Tor, Astrid D. Haase
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
PIWI-interacting RNAs (piRNAs) are regulatory RNAs that bind to PIWI proteins to control transposons and maintain genome integrity. Here the authors characterized their binding specificity and reveal the 5′ nucleotide bias of the Drosophila Piwi pr
Externí odkaz:
https://doaj.org/article/58d34676a5714e7abafef2822ed72676
Publikováno v:
Cell Reports, Vol 3, Iss 6, Pp 1901-1909 (2013)
Argonautes are the central protein component in small RNA silencing pathways. Of the four human Argonautes (hAgo1–hAgo4) only hAgo2 is an active slicer. We determined the structure of hAgo1 bound to endogenous copurified RNAs to 1.75 Å resolution
Externí odkaz:
https://doaj.org/article/3f6f678ec8e34787af29b6ea96cbef9e
Autor:
Astrid D. Haase
Publikováno v:
Stem Cells International, Vol 2016 (2016)
Transposons are mobile genetic elements that threaten the survival of species by destabilizing the germline genomes. Limiting the spread of these selfish elements is imperative. Germ cells employ specialized small regulatory RNA pathways to restrain
Externí odkaz:
https://doaj.org/article/cf246c3f442c43428baa5dd4aad8fb13
Autor:
Daniel Stoyko, Timothy O, Adrianna Hernandez, Parthena Konstantinidou, Qingcai Meng, Astrid D. Haase
Publikováno v:
Current protocolsLiterature Cited. 2(12)
The harnessing of the CRISPR-Cas9 system allows for quick and inexpensive genome editing in tissue culture models. Traditional CRISPR-Cas9 genome editing techniques rely on the ability of single progenitor cells to expand into new pools in a process
Autor:
Constantinos Stathopoulos, Celine Marlin Andrews, Alexandra R Elchert, Amirhossein Manzourolajdad, Pavol Genzor, Parthena Konstantinidou, Daniel Stoyko, Astrid D. Haase
Publikováno v:
Genome Research. 31:2058-2068
Defense against genome invaders universally relies on RNA-guided immunity. Prokaryotic CRISPR/Cas and eukaryotic RNA interference pathways recognize targets by complementary base-pairing, which places the sequences of their guide RNAs at the center o
Autor:
Mamduh Khateb, Jelena Perovanovic, Kyung Dae Ko, Kan Jiang, Xuesong Feng, Natalia Acevedo-Luna, Jérome Chal, Veronica Ciuffoli, Pavol Genzor, James Simone, Astrid D. Haase, Olivier Pourquié, Stefania Dell’Orso, Vittorio Sartorelli
Publikováno v:
Cell reports. 40(7)
Embryonic stem cells (ESCs) can adopt lineage-specific gene-expression programs by stepwise exposure to defined factors, resulting in the generation of functional cell types. Bulk and single-cell-based assays were employed to catalog gene expression,