Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Assaf Pines"'
Publikováno v:
mBio, Vol 8, Iss 6 (2017)
ABSTRACT The standard genetic code is robust to mutations during transcription and translation. Point mutations are likely to be synonymous or to preserve the chemical properties of the original amino acid. Saturation mutagenesis experiments suggest
Externí odkaz:
https://doaj.org/article/2254fccf94434b5795fa8d1833abadee
Publikováno v:
Synthetic biology (Oxford, England). 7(1)
Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set of minimal dege
Publikováno v:
ACS Synthetic Biology. 5:1021-1023
Saturation mutagenesis is widely used in protein engineering and other experiments. A common practice is to utilize the single degenerate codon NNK. However, this approach suffers from amino acid bias and the presence of a stop codon and of the wild
Publikováno v:
ACS synthetic biology. 4(5)
Saturation mutagenesis is employed in protein engineering and genome-editing efforts to generate libraries that span amino acid design space. Traditionally, this is accomplished by using degenerate/compressed codons such as NNK (N = A/C/G/T, K = G/T)