Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Ashwin Seetharaman"'
Autor:
Zhuohan Gu, Chi Wei Ong, Yongzhen Mi, Ashwin Seetharaman, Ryan Ruiyang Ling, Kollengode Ramanathan, Hwa Liang Leo
Publikováno v:
Biomimetics, Vol 8, Iss 6, p 465 (2023)
Heart failure is a global health concern with significant implications for healthcare systems. Left ventricular assist devices (LVADs) provide mechanical support for patients with severe heart failure. However, the placement of the LVAD outflow graft
Externí odkaz:
https://doaj.org/article/b66577b12e304328bf9b22988f9f9d67
Autor:
Traver Hart, Amy Hin Yan Tong, Katie Chan, Jolanda Van Leeuwen, Ashwin Seetharaman, Michael Aregger, Megha Chandrashekhar, Nicole Hustedt, Sahil Seth, Avery Noonan, Andrea Habsid, Olga Sizova, Lyudmila Nedyalkova, Ryan Climie, Leanne Tworzyanski, Keith Lawson, Maria Augusta Sartori, Sabriyeh Alibeh, David Tieu, Sanna Masud, Patricia Mero, Alexander Weiss, Kevin R. Brown, Matej Usaj, Maximilian Billmann, Mahfuzur Rahman, Michael Constanzo, Chad L. Myers, Brenda J. Andrews, Charles Boone, Daniel Durocher, Jason Moffat
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 7, Iss 8, Pp 2719-2727 (2017)
The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used
Externí odkaz:
https://doaj.org/article/d4b448c162324c49bda2645179b12775
Publikováno v:
Bio-Protocol, Vol 5, Iss 7 (2015)
Immunoprecipitation (IP) is a biochemical technique to precipitate a protein out of solution using an antigen that can specifically bind to that protein. IP can be performed to isolate and concentrate one particular protein from a sample of thousands
Externí odkaz:
https://doaj.org/article/1593a244cc024249a1318194fe9785b1
Autor:
Kevin Ka Ming Chan, Ashwin Seetharaman, Rachel Bagg, Guillermo Selman, Yuqian Zhang, Joowan Kim, Peter J Roy
Publikováno v:
PLoS Genetics, Vol 10, Iss 8, p e1004521 (2014)
We recently discovered a secreted and diffusible midline cue called MADD-4 (an ADAMTSL) that guides migrations along the dorsoventral axis of the nematode Caenorhabditis elegans. We showed that the transmembrane receptor, UNC-40 (DCC), whose canonica
Externí odkaz:
https://doaj.org/article/c523b671538f48618c255e00b4c38051
Publikováno v:
Soft Matter. 14:4687-4695
Traction forces exerted by adherent cells are quantified using displacements of embedded markers on polyacrylamide substrates due to cell contractility. Fourier Transform Traction Cytometry (FTTC) is widely used to calculate tractions but has inheren
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::026befe0ab14016e23d061817748a9e2
https://doi.org/10.1039/c7sm02214j
https://doi.org/10.1039/c7sm02214j
Autor:
Ashwin Seetharaman, Hamed Keramati, Kollengode Ramanathan, Matthew E Cove, Sangho Kim, Kian Jon Chua, Hwa Liang Leo
Publikováno v:
Physics of Fluids. 33:061908
Veno-arterial extra corporeal membrane oxygenation (VA-ECMO) is a modified heart lung machine used for patients with both heart and lung failure. This results in retrograde supply of oxygenated blood through the femoral artery in which the unsteady p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eb3ec74e38cda1825c91b2b20b01a096
https://doi.org/10.1063/5.0050962
https://doi.org/10.1063/5.0050962
Autor:
Maximilian Billmann, Avery Noonan, Patricia Mero, Katie Chan, Matej Usaj, Traver Hart, Sabriyeh Alibeh, Jason Moffat, Brenda J. Andrews, Sahil Seth, Jolanda van Leeuwen, Kevin R. Brown, Michael Aregger, Charles Boone, Maria A. Sartori, Ryan Climie, Andrea Habsid, David Tieu, Amy Hin Yan Tong, Leanne Tworzyanski, Keith A. Lawson, Michael Constanzo, Nicole Hustedt, Olga Sizova, Ashwin Seetharaman, Daniel Durocher, Mahfuzur Rahman, Megha Chandrashekhar, Sanna Masud, Chad L. Myers, Alexander Weiss, Lyudmila Nedyalkova
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 7, Iss 8, Pp 2719-2727 (2017)
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics
The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have been used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9457cb31717212a75a809905eade9df1
http://g3journal.org/lookup/doi/10.1534/g3.117.041277
http://g3journal.org/lookup/doi/10.1534/g3.117.041277
Autor:
Donna Tjandra, Frank Sicheri, Noah Manczyk, Maria Augusta Satori, Carsten Schwerdtfeger, Bradley B. Brasher, Sachdev S. Sidhu, Taras Makhnevych, Ashwin Seetharaman, Joan Teyra, Michael Costanzo, Charles Boone, Jason Moffat, Natasha Pascoe
Publikováno v:
Journal of molecular biology. 431(6)
We applied a yeast-two-hybrid (Y2H) analysis to screen for ubiquitin variant (UbV) inhibitors of a human deubiquitinase (DUB), ubiquitin-specific protease 2 (USP2). The Y2H screen used USP2 as the bait and a prey library consisting of UbVs randomized
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c7ab85c1adbe5709e6bc7acf5bd35f5
https://pubmed.ncbi.nlm.nih.gov/30763569
https://pubmed.ncbi.nlm.nih.gov/30763569
Autor:
Lyudmilla Nedyalkova, Megha Chandrashekhar, Maximilian Billmann, Daniel Durocher, Patricia Mero, Ryan Climie, Chad L. Myers, Sanna Masud, Sabriyeh Alibai, Jolanda van Leeuwen, Jason Moffat, Sahil Seth, Traver Hart, Michael Aregger, Avery Noonan, Amy Hin Yan Tong, Brenda J. Andrews, Mahfuzur Rahman, Nicole Hustedt, Katie Chan, Maria A. Sartori, David Tieu, Charles Boone, Alexander Weiss, Keith A. Lawson, Michael Costanzo, Andrea Habsid, Ashwin Seetharaman, Matej Usaj, Kevin R. Brown, Olga Sizova
The adaptation of CRISPR/Cas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs), targeting human protein-coding genes and encoded in viral vectors, have been used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::146c3a00e00b005ed919042fc1ae0bcd
https://doi.org/10.1101/117341
https://doi.org/10.1101/117341
Autor:
Rachel Puckrin, Eric Wong, Ashwin Seetharaman, Serena A. D’Souza, Guillermo Selman, Peter J. Roy, Louis Barbier
Publikováno v:
Developmental Cell. 21:669-680
SummaryThe netrins and slits are two families of widely conserved cues that guide axons and cells along the dorsal-ventral (D-V) axis of animals. These cues typically emanate from the dorsal or ventral midlines and provide spatial information to migr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06a3dd26529efb620f8f6b37c9f42f12
https://doi.org/10.1016/j.devcel.2011.07.020
https://doi.org/10.1016/j.devcel.2011.07.020