Zobrazeno 1 - 10
of 42
pro vyhledávání: '"Ashley M. Jacobi"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-10 (2023)
The generation of CRISPR-mediated transcriptional activation (CRISPRa)-competent cell lines pose significant technical challenges. Here the authors report a platform for production of CRISPRa-ready cell populations which they combine with optimised e
Externí odkaz:
https://doaj.org/article/d41507a002324e478b140f579db08654
Autor:
Jonas Kath, Weijie Du, Alina Pruene, Tobias Braun, Bernice Thommandru, Rolf Turk, Morgan L. Sturgeon, Gavin L. Kurgan, Leila Amini, Maik Stein, Tatiana Zittel, Stefania Martini, Lennard Ostendorf, Andreas Wilhelm, Levent Akyüz, Armin Rehm, Uta E. Höpken, Axel Pruß, Annette Künkele, Ashley M. Jacobi, Hans-Dieter Volk, Michael Schmueck-Henneresse, Renata Stripecke, Petra Reinke, Dimitrios L. Wagner
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 25, Iss , Pp 311-330 (2022)
Chimeric antigen receptor (CAR) redirected T cells are potent therapeutic options against hematological malignancies. The current dominant manufacturing approach for CAR T cells depends on retroviral transduction. With the advent of gene editing, ins
Externí odkaz:
https://doaj.org/article/007e0c658baf459b90751503a142b022
Autor:
Gavin Kurgan, Rolf Turk, Heng Li, Nathan Roberts, Garrett R. Rettig, Ashley M. Jacobi, Lauren Tso, Morgan Sturgeon, Massimo Mertens, Roel Noten, Kurt Florus, Mark A. Behlke, Yu Wang, Matthew S. McNeill
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss , Pp 478-491 (2021)
CRISPR systems enable targeted genome editing in a wide variety of organisms by introducing single- or double-strand DNA breaks, which are repaired using endogenous molecular pathways. Characterization of on- and off-target editing events from CRISPR
Externí odkaz:
https://doaj.org/article/5e209aed7e8a4ef2be494827ee71b257
Autor:
Jenny Shapiro, Ortal Iancu, Ashley M. Jacobi, Matthew S. McNeill, Rolf Turk, Garrett R. Rettig, Ido Amit, Adi Tovin-Recht, Zohar Yakhini, Mark A. Behlke, Ayal Hendel
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 17, Iss , Pp 1097-1107 (2020)
Genome editing of human cluster of differentiation 34+ (CD34+) hematopoietic stem and progenitor cells (HSPCs) holds great therapeutic potential. This study aimed to optimize on-target, ex vivo genome editing using the CRISPR-Cas9 system in CD34+ HSP
Externí odkaz:
https://doaj.org/article/74189cca19364566b5cb4597df8b7115
Autor:
Rebecca L. Kow, Timothy J. Strovas, Pamela J. McMillan, Ashley M. Jacobi, Mark A. Behlke, Aleen D. Saxton, Caitlin S. Latimer, C. Dirk Keene, Brian C. Kraemer
Publikováno v:
Neurobiology of Disease, Vol 147, Iss , Pp 105148- (2021)
Aging drives pathological accumulation of proteins such as tau, causing neurodegenerative dementia disorders like Alzheimer's disease. Previously we showed loss of function mutations in the gene encoding the poly(A) RNA binding protein SUT-2/MSUT2 su
Externí odkaz:
https://doaj.org/article/a306260c5f1f44a8881453e339558866
Autor:
Rolen M. Quadros, Hiromi Miura, Donald W. Harms, Hisako Akatsuka, Takehito Sato, Tomomi Aida, Ronald Redder, Guy P. Richardson, Yutaka Inagaki, Daisuke Sakai, Shannon M. Buckley, Parthasarathy Seshacharyulu, Surinder K. Batra, Mark A. Behlke, Sarah A. Zeiner, Ashley M. Jacobi, Yayoi Izu, Wallace B. Thoreson, Lisa D. Urness, Suzanne L. Mansour, Masato Ohtsuka, Channabasavaiah B. Gurumurthy
Publikováno v:
Genome Biology, Vol 18, Iss 1, Pp 1-15 (2017)
Abstract Background Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice a
Externí odkaz:
https://doaj.org/article/cc09dd43b09b4403b9ad7415943adf1e
Autor:
Pooran Singh Dewari, Benjamin Southgate, Katrina Mccarten, German Monogarov, Eoghan O'Duibhir, Niall Quinn, Ashley Tyrer, Marie-Christin Leitner, Colin Plumb, Maria Kalantzaki, Carla Blin, Rebecca Finch, Raul Bardini Bressan, Gillian Morrison, Ashley M Jacobi, Mark A Behlke, Alex von Kriegsheim, Simon Tomlinson, Jeroen Krijgsveld, Steven M Pollard
Publikováno v:
eLife, Vol 7 (2018)
CRISPR/Cas9 can be used for precise genetic knock-in of epitope tags into endogenous genes, simplifying experimental analysis of protein function. However, Cas9-assisted epitope tagging in primary mammalian cell cultures is often inefficient and reli
Externí odkaz:
https://doaj.org/article/75965eb632694dcb913174d34eba59b9
CRISPR-mediated transcriptional activation (CRISPRa) is a powerful technology for inducing gene expression from endogenous loci with exciting applications in high throughput gain-of-function genomic screens and the engineering of cell-based models. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8df10e1ded7b3e7916f1da375938c817
https://doi.org/10.1101/2022.07.21.501015
https://doi.org/10.1101/2022.07.21.501015
Autor:
Mark A. Behlke, Yu Wang, Lauren Tso, Nathan Roberts, Matthew S. McNeill, Gavin Kurgan, Morgan Sturgeon, Heng Li, Roel Noten, Ashley M. Jacobi, Massimo Mertens, Garrett R. Rettig, Kurt Florus, Rolf Turk
Publikováno v:
Molecular Therapy. Methods & Clinical Development
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss, Pp 478-491 (2021)
Molecular Therapy: Methods & Clinical Development, Vol 21, Iss, Pp 478-491 (2021)
CRISPR systems enable targeted genome editing in a wide variety of organisms by introducing single- or double-strand DNA breaks, which are repaired using endogenous molecular pathways. Characterization of on- and off-target editing events from CRISPR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb0e71b10793ebd9a5eee2a52efcf87a
http://europepmc.org/articles/PMC8082044
http://europepmc.org/articles/PMC8082044
Autor:
Mark A. Behlke, Adi Tovin-Recht, Jenny Shapiro, Ayal Hendel, Ashley M. Jacobi, Matthew S. McNeill, Zohar Yakhini, Garrett R. Rettig, Ido Amit, Rolf Turk, Ortal Iancu
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 17, Iss, Pp 1097-1107 (2020)
Molecular Therapy-Methods & Clinical Development
Molecular Therapy. Methods & Clinical Development
Molecular Therapy-Methods & Clinical Development
Molecular Therapy. Methods & Clinical Development
Genome editing of human cluster of differentiation 34+ (CD34+) hematopoietic stem and progenitor cells (HSPCs) holds great therapeutic potential. This study aimed to optimize on-target, ex vivo genome editing using the CRISPR-Cas9 system in CD34+ HSP
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::baca97288c11dd29a6cc0f972da0d999
http://www.sciencedirect.com/science/article/pii/S2329050120300875
http://www.sciencedirect.com/science/article/pii/S2329050120300875