An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells

Autor: Daniel J. Browne, Jamie L. Brady, Ashley J. Waardenberg, Claire Loiseau, Denise L. Doolan

Publikováno v: Frontiers in Immunology, Vol 11 (2020)

Reliable extraction and sensitive detection of RNA from human peripheral blood mononuclear cells (PBMCs) is critical for a broad spectrum of immunology research and clinical diagnostics. RNA analysis platforms are dependent upon high-quality and high

Externí odkaz: https://doaj.org/article/2e9ff1c6d5834441a797206f31b815ca

Prediction and validation of protein–protein interactors from genome-wide DNA-binding data using a knowledge-based machine-learning approach

Autor: Ashley J. Waardenberg, Bernou Homan, Stephanie Mohamed, Richard P. Harvey, Romaric Bouveret

Publikováno v: Open Biology, Vol 6, Iss 9 (2016)

The ability to accurately predict the DNA targets and interacting cofactors of transcriptional regulators from genome-wide data can significantly advance our understanding of gene regulatory networks. NKX2-5 is a homeodomain transcription factor that

Externí odkaz: https://doaj.org/article/e99937f145bc4f0dbe25c2bec1f0cce8

A presynaptic phosphosignaling hub for lasting homeostatic plasticity

Autor: Johannes Alexander Müller, Julia Betzin, Jorge Santos-Tejedor, Annika Mayer, Ana-Maria Oprişoreanu, Kasper Engholm-Keller, Isabelle Paulußen, Polina Gulakova, Terrence Daniel McGovern, Lena Johanna Gschossman, Eva Schönhense, Jesse R. Wark, Alf Lamprecht, Albert J. Becker, Ashley J. Waardenberg, Mark E. Graham, Dirk Dietrich, Susanne Schoch

Publikováno v: Müller, J A, Betzin, J, Santos-Tejedor, J, Mayer, A, Oprişoreanu, A M, Engholm-Keller, K, Paulußen, I, Gulakova, P, McGovern, T D, Gschossman, L J, Schönhense, E, Wark, J R, Lamprecht, A, Becker, A J, Waardenberg, A J, Graham, M E, Dietrich, D & Schoch, S 2022, ' A presynaptic phosphosignaling hub for lasting homeostatic plasticity ', Cell Reports, vol. 39, no. 3, 110696 . https://doi.org/10.1016/j.celrep.2022.110696

Stable function of networks requires that synapses adapt their strength to levels of neuronal activity, and failure to do so results in cognitive disorders. How such homeostatic regulation may be implemented in mammalian synapses remains poorly under

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1076f6fbeab7fdab5a24092caefb8a9b
https://findresearcher.sdu.dk:8443/ws/files/201891353/1_s2.0_S2211124722004545_main.pdf

NKX2-5 mutations causative for congenital heart disease retain functionality and are directed to hundreds of targets

Autor: Romaric Bouveret, Ashley J Waardenberg, Nicole Schonrock, Mirana Ramialison, Tram Doan, Danielle de Jong, Antoine Bondue, Gurpreet Kaur, Stephanie Mohamed, Hananeh Fonoudi, Chiann-mun Chen, Merridee A Wouters, Shoumo Bhattacharya, Nicolas Plachta, Sally L Dunwoodie, Gavin Chapman, Cédric Blanpain, Richard P Harvey

Publikováno v: eLife, Vol 4 (2015)

We take a functional genomics approach to congenital heart disease mechanism. We used DamID to establish a robust set of target genes for NKX2-5 wild type and disease associated NKX2-5 mutations to model loss-of-function in gene regulatory networks.

Externí odkaz: https://doaj.org/article/4af17a74739c4c6ca56af49e332c7b67

TWIST1 Homodimers and Heterodimers Orchestrate Lineage-Specific Differentiation

Autor: David A.F. Loebel, Mark E. Graham, Nicolas Fossat, Madeleine Demuth, Patrick P.L. Tam, Xiaochen Fan, Pierre Osteil, Jane Sun, Ashley J. Waardenberg

Publikováno v: Mol Cell Biol

The extensive array of basic helix-loop-helix (bHLH) transcription factors and their combinations as dimers underpin the diversity of molecular function required for cell type specification during embryogenesis. The bHLH factor TWIST1 plays pleiotrop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d55adc6439e157379e0a5611adcca05c
https://pubmed.ncbi.nlm.nih.gov/32179550