Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Arvydas Janulaitis"'
Autor:
Arvydas Janulaitis, Robertas Galinis, Linas Mazutis, Vaidotas Kiseliovas, Sabine Studer, Rapolas Zilionis, Donald Hilvert, Greta Stonyte
Publikováno v:
Angewandte Chemie (International Ed. in English)
The amplification and digital quantification of single DNA molecules are important in biomedicine and diagnostics. Beyond quantifying DNA molecules in a sample, the ability to express proteins from the amplified DNA would open even broader applicatio
Autor:
Arvydas Janulaitis
Publikováno v:
Modern Biopharmaceuticals
Autor:
Arunas Lagunavicius, Arvydas Janulaitis, Egle Merkiene, Laurynas Riauba, Edita Gaidamaviciute
Publikováno v:
RNA. 16:1508-1515
We improved the target RNA-primed RCA technique for direct detection and analysis of RNA in vitro and in situ. Previously we showed that the 3′ → 5′ single-stranded RNA exonucleolytic activity of Phi29 DNA polymerase converts the target RNA int
Autor:
Tomas Radzvilavicius, Agne Savaneviciute, Zivile Kiveryte, Egle Merkiene, Arvydas Janulaitis, Vilma Zimbaite-Ruskuliene, Arunas Lagunavicius
Publikováno v:
RNA. 15:765-771
We present a novel Phi29 DNA polymerase application in RCA-based target RNA detection and analysis. The 3′→5′ RNase activity of Phi29 DNA polymerase converts target RNA into a primer and the polymerase uses this newly generated primer for RCA i
Publikováno v:
Journal of Molecular Biology. 370:157-169
Type IIS restriction endonuclease Eco31I is a "short-distance cutter", which cleaves DNA strands close to its recognition sequence, 5'-GGTCTC(1/5). Previously, it has been proposed that related endonucleases recognizing a common sequence core GTCTC p
Autor:
Grzegorz Bujacz, Mariusz Jaskolski, Saulius Grazulis, Robert Janowski, Giedre Tamulaitiene, Arvydas Janulaitis
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1698:251-254
Restriction endonuclease Eco57I from Escherichia coli recognizes asymmetric DNA sequence 5′-CTGAAG and has both restriction (DNA cleavage a short distance away from the recognition site) and modification (methylation) activities residing in a singl
Autor:
Arvydas Janulaitis, Arvydas Lubys, Z. Maneliene, R Kazlauskiene, Albertas Timinskas, M Petrusyte, E Cesnaviciene
Publikováno v:
Journal of Molecular Biology. 314:205-216
We report the properties of the new AloI restriction and modification enzyme from Acinetobacter lwoffi Ks 4-8 that recognizes the DNA target 5' GGA(N)6GTTC3' (complementary strand 5' GAAC(N)6TCC3'), and the nucleotide sequence of the gene encoding th
Publikováno v:
FEMS Microbiology Letters. 204:253-257
One hundred and forty isolates of thermophilic bacteria from the genus Thermus were screened for the presence of restriction endonuclease activity. Thermostable isoschizomers of restriction endonucleases, such as AceIII, BbvI, BglI, BsePI, FnuDII, Hg
Autor:
Virginija Urbelyte, Arvydas Janulaitis, Arvydas Lubys, Sonata Jurenaite-Urbanaviciene, Z. Maneliene, Ruta Kazlauskiene, M. Petrusyte
Publikováno v:
Nucleic Acids Research. 29:895-903
We report the properties of the new BseMII restriction and modification enzymes from Bacillus stearothermophilus Isl 15-111, which recognize the 5′-CTCAG sequence, and the nucleotide sequence of the genes encoding them. The restriction endonuclease
Publikováno v:
Nucleic Acids Research. 27:4228-4234
Kpn 2I enzymes of a type II restriction-modification (R-M) system from the bacterium Klebsiella pneumoniae strain RFL2 recognize the sequence 5'-TCCGGA-3'. The Kpn 2I R-M genes have been cloned and expressed in Escherichia coli. DNA sequence analysis