Analysis of polar urinary metabolites for metabolic phenotyping using supercritical fluid chromatography and mass spectrometry

Autor: Jeremy K. Nicholson, Ian D. Wilson, Matthew R. Lewis, Christopher Knappy, Robert S. Plumb, Norman W. Smith, Arundhuti Sen

Publikováno v: Sen, A, Knappy, C, Lewis, M R, Plumb, R S, Wilson, I D, Nicholson, J K & Smith, N W 2016, ' Analysis of polar urinary metabolites for metabolic phenotyping using supercritical fluid chromatography and mass spectrometry ', Journal of Chromatography A, vol. 1449, pp. 141–155 . https://doi.org/10.1016/j.chroma.2016.04.040
Journal of Chromatography. a

Highlights • An SFC method has been developed for the analysis of polar urinary metabolites. • 12 stationary phases, 9 modifier additives and 3 temperatures were evaluated. • DIOL and 2-PIC columns provide highest peak capacity and overall reso

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fae5050d449f9f19da036a8635e3efa5

Extension and Limits of the Network of Coupled Motions Correlated to Hydride Transfer in Dihydrofolate Reductase

Autor: Priyanka Singh, Arundhuti Sen, Kevin Francis, Amnon Kohen

Publikováno v: Journal of the American Chemical Society

Enzyme catalysis has been studied extensively, but the role of enzyme dynamics in the catalyzed chemical conversion is still an enigma. The enzyme dihydrofolate reductase (DHFR) is often used as a model system to assess a network of coupled motions a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ce7886d99002f511a362842b3da104f
https://doi.org/10.1021/ja411998h

Lipidomics comparing DCD and DBD liver allografts uncovers lysophospholipids elevated in recipients undergoing early allograft dysfunction

Autor: Petroula Proitsi, Maltina Shkodra, Ana María Casas-Ferreira, P. Srinivasan, Maria Tena, Nigel Heaton, Arundhuti Sen, Yun Ma, Jin Xu, Wayel Jassem, Cristina Legido-Quigley, Min Kim

Publikováno v: Scientific Reports
Xu, J, Casas-Ferreira, A M, Ma, Y, Sen, A, Kim, M, Proitsi, P, Shkodra, M, Tena, M, Srinivasan, P, Heaton, N, Jassem, W & Legido-Quigley, C 2015, ' Lipidomics comparing DCD and DBD liver allografts uncovers lysophospholipids elevated in recipients undergoing early allograft dysfunction ', Scientific Reports, vol. 5, 17737 . https://doi.org/10.1038/srep17737

Finding specific biomarkers of liver damage in clinical evaluations could increase the pool of available organs for transplantation. Lipids are key regulators in cell necrosis and hence this study hypothesised that lipid levels could be altered in or

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4240304dcd555f867d91b319be8bc334
http://europepmc.org/articles/PMC4669413

Microscale synthesis and kinetic isotope effect analysis of (4R)-[Ad-14C,4-2H] NADPH and (4R)-[Ad-3H,4-2H] NADPH

Autor: Amnon Kohen, Arundhuti Sen, Atsushi Yahashiri

Publikováno v: Journal of Labelled Compounds and Radiopharmaceuticals. 52:463-466

We present a one-pot chemo-enzymatic microscale synthesis of NADPH with two different patterns of isotopic labels: (4R)-[Ad-14C,4-2H] NADPH and (4R)-[Ad-3H,4-2H] NADPH. These co-factors are required by an enormous range of enzymes, and isotopically l

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7f6eb7e3bfae43508eec751b260dc1b1
https://doi.org/10.1002/jlcr.1660

A [2+2] cross-photodimerisation of photostable olefins via a three-component cocrystal solid solution

Autor: Dejan-Krešimir Bučar, Arundhuti Sen, Leonard R. MacGillivray, S. V. Santhana Mariappan

Publikováno v: Chemical communications (Cambridge, England). 48(12)

A ditopic hydrogen-bond-donor template in the form of resorcinol facilitates a [2+2] cross-photodimerisation of 4-Cl-stilbazole and 4-Me-stilbazole in a rare cocrystal solid solution. A photoreaction does not proceed with the olefins individually or

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e9f5b94a15bd0103a6a7ead413b6081
https://pubmed.ncbi.nlm.nih.gov/22215020