Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells

Autor: Greta Bigelyte, Joshua K. Young, Tautvydas Karvelis, Karolina Budre, Rimante Zedaveinyte, Vesna Djukanovic, Elizabeth Van Ginkel, Sushmitha Paulraj, Stephen Gasior, Spencer Jones, Lanie Feigenbutz, Grace St. Clair, Pierluigi Barone, Jennifer Bohn, Ananta Acharya, Gina Zastrow-Hayes, Selgar Henkel-Heinecke, Arunas Silanskas, Ralf Seidel, Virginijus Siksnys

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)

Miniature Cas12f editing systems are well suited for in vivo editing applications. Here the authors characterize the intrinsic activity of SpCas12f1 in plant and animal cells.

Externí odkaz: https://doaj.org/article/688a63ea2c9f45e084a40d762048f444

TnpB structure reveals minimal functional core of Cas12 nuclease family

Autor: Giedrius Sasnauskas, Giedre Tamulaitiene, Gytis Druteika, Arturo Carabias, Arunas Silanskas, Darius Kazlauskas, Česlovas Venclovas, Guillermo Montoya, Tautvydas Karvelis, Virginijus Siksnys

Publikováno v: Nature. 616:384-389

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5f99d9770164b819dc9bc32b47fb4fbb
https://doi.org/10.1038/s41586-023-05826-x

Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease

Autor: Česlovas Venclovas, Gytis Druteika, Darius Kazlauskas, Arunas Silanskas, Virginijus Siksnys, Rimante Zedaveinyte, Karolina Budre, Tautvydas Karvelis, Greta Bigelyte

Publikováno v: Nature, Berlin : Nature research, 2021, vol. 599, p. 692-696

Transposition has a key role in reshaping genomes of all living organisms1. Insertion sequences of IS200/IS605 and IS607 families2 are among the simplest mobile genetic elements and contain only the genes that are required for their transposition and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51bf5a25af0ec559da51d1bb5a09fafe
https://doi.org/10.1038/s41586-021-04058-1

Short prokaryotic Argonautes provide defence against incoming mobile genetic elements through NAD+ depletion

Autor: Mindaugas Zaremba, Donata Dakineviciene, Edvardas Golovinas, Evelina Zagorskaitė, Edvinas Stankunas, Anna Lopatina, Rotem Sorek, Elena Manakova, Audrone Ruksenaite, Arunas Silanskas, Simonas Asmontas, Algirdas Grybauskas, Ugne Tylenyte, Edvinas Jurgelaitis, Rokas Grigaitis, Kęstutis Timinskas, Česlovas Venclovas, Virginijus Siksnys

Publikováno v: Nature microbiology 7(11), 1857-1869 (2022). doi:10.1038/s41564-022-01239-0

Nature microbiology 7(11), 1857 - 1869 (2022). doi:10.1038/s41564-022-01239-0
Argonaute (Ago) proteins are found in all three domains of life. The so-called long Agos are composed of four major domains (N, PAZ, MID and PIWI) and contribute to RN

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3be66bd03d24d2cf5ff56bbe4bca24e7
https://bib-pubdb1.desy.de/record/485697

Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer

Autor: Arunas Silanskas, Edvardas Golovinas, Giedrius Sasnauskas, Mindaugas Zaremba, Danielis Rutkauskas, Marija Jankunec, Elena Manakova

Publikováno v: Scientific reports, London : Nature, 2021, vol. 11, art. no. 4518, p. 1-14
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
Scientific reports 11(1), 4518 (2021). doi:10.1038/s41598-021-83889-4
Scientific Reports

Scientific reports 11(1), 4518 (2021). doi:10.1038/s41598-021-83889-4
Argonaute (Ago) proteins are found in all three domains of life. The best-characterized group is eukaryotic Argonautes (eAgos), which are the core of RNA interference. The bes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3af00b310d7d191c96583f63af590394
https://repository.vu.lt/VU:ELABAPDB103072966&prefLang=en_US