Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Arunas Lagunavicius"'
Publikováno v:
PLoS ONE, Vol 11, Iss 3, p e0150404 (2016)
In our previous work we showed that DNaseI-like protein from an extremely halotolerant bacterium Thioalkalivibrio sp. K90mix retained its activity at salt concentrations as high as 4 M NaCl and the key factor allowing this was the C-terminal DNA-bind
Externí odkaz:
https://doaj.org/article/203fc9a06bc74586a3b0d45da452569f
Autor:
Gediminas Alzbutas, Eimantas Astromskas, Arunas Lagunavicius, Renata Bružaitė, Milda Kaniušaitė
Publikováno v:
Nature Methods. 12:i-iii
The Scientific™ EpiJET™ 5-hmC Enrichment Kit is highly specific for different DNA samples containing 5-hydroxymethylcytosine (5-hmC), an extensively studied DNA epigenetic modification. This tool, when combined with next-generation sequencing, of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::df7a4740c50258a4406ea921570d76b3
https://doi.org/10.1038/nmeth.f.377
https://doi.org/10.1038/nmeth.f.377
Publikováno v:
New Biotechnology. 29:285-292
Moloney murine leukemia virus reverse transcriptase (M-MuLV RT) is a domain structured enzyme that has the N-terminally located DNA polymerization activity and C-terminally located RNase H activity, which interferes with the efficient synthesis of lo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c143b311cf9dee2091908cf7ad42e4ac
https://doi.org/10.1016/j.nbt.2011.07.004
https://doi.org/10.1016/j.nbt.2011.07.004
Autor:
Arunas Lagunavicius, Arvydas Janulaitis, Egle Merkiene, Laurynas Riauba, Edita Gaidamaviciute
Publikováno v:
RNA. 16:1508-1515
We improved the target RNA-primed RCA technique for direct detection and analysis of RNA in vitro and in situ. Previously we showed that the 3′ → 5′ single-stranded RNA exonucleolytic activity of Phi29 DNA polymerase converts the target RNA int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1245e1a877b28787e3d2a314448ecbaf
https://doi.org/10.1261/rna.2068510
https://doi.org/10.1261/rna.2068510
Autor:
Tomas Radzvilavicius, Agne Savaneviciute, Zivile Kiveryte, Egle Merkiene, Arvydas Janulaitis, Vilma Zimbaite-Ruskuliene, Arunas Lagunavicius
Publikováno v:
RNA. 15:765-771
We present a novel Phi29 DNA polymerase application in RCA-based target RNA detection and analysis. The 3′→5′ RNase activity of Phi29 DNA polymerase converts target RNA into a primer and the polymerase uses this newly generated primer for RCA i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd22d98cb8d53e9aa93529536335d3d1
https://doi.org/10.1261/rna.1279909
https://doi.org/10.1261/rna.1279909
Autor:
Česlovas Venclovas, Claus Urbanke, Kornelijus Stankevicius, Arunas Lagunavicius, Rasa Sukackaite, Virginijus Siksnys
Publikováno v:
Nucleic Acids Research
Type IIS restriction endonucleases (REases) recognize asymmetric DNA sequences and cleave both DNA strands at fixed positions downstream of the recognition site. REase BpuJI recognizes the asymmetric sequence 5′-CCCGT, however it cuts at multiple s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::037e093f4648c5dfa82c2d253624fa9a
https://doi.org/10.1093/nar/gkm164
https://doi.org/10.1093/nar/gkm164
Publikováno v:
Frontiers in Microbiology, Vol 6 (2015)
Frontiers in Microbiology
Frontiers in Microbiology, Lausanne : Frontiers Research Foundation, 2015, Vol. 6, Art. No. 661
Frontiers in Microbiology
Frontiers in Microbiology, Lausanne : Frontiers Research Foundation, 2015, Vol. 6, Art. No. 661
Our study indicates that DNA binding domains are common in many halophilic or halotolerant bacterial DNases and they are potential activators of enzymatic activity at high ionic strength. Usually, proteins adapt to high ionic strength by increasing t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb8fd7eb85715e4a1caa78847de6f102
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00661/full
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00661/full
Autor:
Saulius Serva, Arunas Lagunavicius
Publikováno v:
Bioconjugate chemistry. 26(6)
Recent discovery of functional 5-hydroxymethylcytosine in vertebrate genomes prompted for elaboration of methods to localize this modification at the nucleotide resolution level. Among several covalent modification-based approaches, atypical activity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ceacc8abe9391d13909dea875e74f2b
https://pubmed.ncbi.nlm.nih.gov/25982286
https://pubmed.ncbi.nlm.nih.gov/25982286
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1751:194-204
The Type IIS restriction endonuclease MnlI recognizes the non-palindromic nucleotide sequence 5'-CCTC(N)7/6 downward arrow and cleaves DNA strands as indicated by the arrow. The genes encoding MnlI restriction-modification system were cloned and sequ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7506e068928fb9a9496fb470cf2c5c0
https://doi.org/10.1016/j.bbapap.2005.06.006
https://doi.org/10.1016/j.bbapap.2005.06.006
Publikováno v:
Biochemistry. 40:14960-14967
To investigate the effect of pH on sequence-specific binding, a thermodynamic characterization of the interaction of the protein MunI with a specific, and a nonspecific, oligonucleotide was performed. MunI is a type II restriction endonuclease which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbb21c08fe2cf0b76bd063c6056322c1
https://doi.org/10.1021/bi0113566
https://doi.org/10.1021/bi0113566