Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Arthur M. Hanel"'
SITE-SPECIFIC LABELING OF A PROTEIN LYSINE RESIDUE BY NOVEL KINETIC LABELING COMBINATORIAL LIBRARIES
Autor:
Allen Krantz, Arthur M Hanel, Ivona Strug, Andrzej Wilczynski, Jeremy J Wolff, Wolin Huang, Linda H Huang, Tina Settineri, Darren L Holmes, Margaret C Hardy, Dominique P Bridon
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 9, Iss 15 (2014)
The first example of a kinetic labeling library designed to enable the discovery of affinity labels is presented. Each library component (1) consists of a variable peptidyl component linked to a biotinyl moiety by a 4-mercaptobenzoyl linker in thioes
Externí odkaz:
https://doaj.org/article/b5781b56027b45358e4cbb98b9eca02e
Publikováno v:
Annual Review of Biochemistry. 64:653-688
Interfacial enzymes operate at an organized interface such as lipid aggregates in contact with the aqueous phase. The enzyme phospholipase A2 is a well studied interfacial enzyme, and a discussion of its behavior at interfaces is the topic of this re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::232696365a6ca44ec04dd0d64728a4db
https://doi.org/10.1146/annurev.bi.64.070195.003253
https://doi.org/10.1146/annurev.bi.64.070195.003253
Autor:
William P. Jencks, Arthur M. Hanel
Publikováno v:
Biochemistry. 30:11320-11330
The internalization of 45Ca by the calcium-transporting ATPase into sarcoplasmic reticulum vesicles from rabbit muscle was measured during a single turnover of the enzyme by using a quench of 7 mM ADP and EGTA (25 degrees C, 5 mM MgCl2, 100 mM KCl, 4
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e5908aa08056cf334e0fc00d88f63eb
https://doi.org/10.1021/bi00111a019
https://doi.org/10.1021/bi00111a019
SITE-SPECIFIC LABELING OF A PROTEIN LYSINE RESIDUE BY NOVEL KINETIC LABELING COMBINATORIAL LIBRARIES
Autor:
Darren L. Holmes, Allen Krantz, Margaret Hardy, Andrzej Wilczynski, Arthur M. Hanel, Wolin Huang, Dominique P. Bridon, Linda H Huang, Tina Settineri, Ivona Strug, Jeremy J. Wolff
Publikováno v:
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal, Vol 9, Iss 15 (2014)
Computational and Structural Biotechnology Journal, Vol 9, Iss 15 (2014)
The first example of a kinetic labeling library designed to enable the discovery of affinity labels is presented. Each library component (1) consists of a variable peptidyl component linked to a biotinyl moiety by a 4-mercaptobenzoyl linker in thioes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18bdd529890f77e81d031117a5e6f8e3
https://doi.org/10.5936/csbj.201403001
https://doi.org/10.5936/csbj.201403001
Publikováno v:
Analytical biochemistry. 270(1)
Antithrombotic and clotting factors have long been targets for drug discovery, necessitating the development of blood assays to determine the efficacy of lead compounds prior to animal testing. We have developed a reconstituted blood clot lysis assay
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::582433184cd33d21dbf8b3e76d9e6033
https://pubmed.ncbi.nlm.nih.gov/10328761
https://pubmed.ncbi.nlm.nih.gov/10328761
Autor:
Arthur M. Hanel, Michael H. Gelb
Publikováno v:
Biochemistry. 34(24)
The recombinant human 85-kDa cytosolic phospholipase A2 (cPLA2), when assayed in the presence of glycerol, catalyzes the transfer of acyl chains of radiolabeled phosphatidylcholine and para-substituted phenyl esters of fatty acids to glycerol, in add
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0bc738a7d9a328c2b2fb46ac6546b49b
https://pubmed.ncbi.nlm.nih.gov/7794891
https://pubmed.ncbi.nlm.nih.gov/7794891
Publikováno v:
Biochemistry. 32(23)
Substrate specificities of the human and rat kidney 85-kDa phospholipase A2 enzymes (hmw-PLA2) have been determined under conditions in which hydrolysis of substrate vesicles occurs without the desorption of enzyme from the interface (scooting mode c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee385288086b9a7cb4766034ddc390d3
https://pubmed.ncbi.nlm.nih.gov/8507635
https://pubmed.ncbi.nlm.nih.gov/8507635
Autor:
William P. Jencks, Arthur M. Hanel
Publikováno v:
Biochemistry. 29(21)
The calcium-transport ATPase (CaATPase) of rabbit sarcoplasmic reticulum preincubated with 0.02 mM Ca2+ (cE.Ca2) is phosphorylated upon the addition of 0.25 mM LaCl3 and 0.3 mM [gamma-32P]ATP with an observed rate constant of 6.5 s-1 (40 mM MOPS, pH
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5aecc6d9b634d6736b94b755ede60478
https://pubmed.ncbi.nlm.nih.gov/2143081
https://pubmed.ncbi.nlm.nih.gov/2143081
Autor:
Arthur M. Hanel, William E.M. Lands
Publikováno v:
Prostaglandins. 24:271-277
This report demonstrates that a non-steroidal antiinflammatory drug, MK 447, and phenol act similarly to inhibit cyclooxygenase activity in vitro in a dose-dependent manner when the concentration of peroxide activators is decreased by glutathione per
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f15b75938ae4923b8e3a14f0ad496743
https://doi.org/10.1016/0090-6980(82)90153-8
https://doi.org/10.1016/0090-6980(82)90153-8
Autor:
William E.M. Lands, Arthur M. Hanel
Publisher Summary This chapter discusses the inhibitors and activators of prostaglandin biosynthesis. Peroxidase cosubstrates, particularly phenolic agents, have been reported to increase the synthesis of prostaglandins in vitro . This activation of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::73fa9452fb00fe0de57f870b7a3f83fb
https://doi.org/10.1016/s0167-7306(08)60538-4
https://doi.org/10.1016/s0167-7306(08)60538-4
