Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Aron Broom"'
Ensemble-based enzyme design can recapitulate the effects of laboratory directed evolution in silico
Autor:
Aron Broom, Rojo V. Rakotoharisoa, Michael C. Thompson, Niayesh Zarifi, Erin Nguyen, Nurzhan Mukhametzhanov, Lin Liu, James S. Fraser, Roberto A. Chica
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Kemp eliminases are artificial enzymes that catalyze the concerted deprotonation and ring-opening of benzisoxazoles. Here, the authors use room-temperature X-ray crystallography to investigate changes to the conformational ensemble of the Kemp elimin
Externí odkaz:
https://doaj.org/article/7439f4de0efc41d198a2176b909a8ff0
Autor:
Duncan W. S. MacKenzie, Anna Schaefer, Julia Steckner, Christopher A. Leo, Dalia Naser, Efrosini Artikis, Aron Broom, Travis Ko, Purnank Shah, Mikaela Q. Ney, Elisa Tran, Martin T. J. Smith, Brian Fuglestad, A. Joshua Wand, Charles L. Brooks, Elizabeth M. Meiering
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America. 119(26)
Allostery is the phenomenon of coupling between distal binding sites in a protein. Such coupling is at the crux of protein function and regulation in a myriad of scenarios, yet determining the molecular mechanisms of coupling networks in proteins rem
Autor:
Michael Ong, Bryan Lo, Marie-Ève Wedge, Carolina S. Ilkow, John C. Bell, Catia Cemeus, Oliver Varette, Aron Broom, Benjamin McSweeney, Bruce F. Burns, Harold L. Atkins, Brian J Laight, Julia Petryk, Brian A. Keller, Jean-Simon Diallo, Carolyn Nessim, Roberto A. Chica
Publikováno v:
Journal of Cancer Research and Clinical Oncology. 147:1365-1378
Mutations in BRAF are the most prominent activating mutations in melanoma and are increasingly recognized in other cancers. There is currently no accepted treatment regimen for patients with mutant BRAFK601N melanoma, and the study of melanoma driven
Autor:
Brian A, Keller, Brian J, Laight, Oliver, Varette, Aron, Broom, Marie-Ève, Wedge, Benjamin, McSweeney, Catia, Cemeus, Julia, Petryk, Bryan, Lo, Bruce, Burns, Carolyn, Nessim, Michael, Ong, Roberto A, Chica, Harold L, Atkins, Jean-Simon, Diallo, Carolina S, Ilkow, John C, Bell
Publikováno v:
Journal of cancer research and clinical oncology. 147(5)
Mutations in BRAF are the most prominent activating mutations in melanoma and are increasingly recognized in other cancers. There is currently no accepted treatment regimen for patients with mutant BRAFWe developed and characterized the first patient
Ensemble-based enzyme design can recapitulate the effects of laboratory directed evolution in silico
Autor:
Lin Liu, Michael C. Thompson, Niayesh Zarifi, Erin Nguyen, Aron Broom, Rojo V Rakotoharisoa, Nurzhan Mukhametzhanov, James S. Fraser, Roberto A. Chica
Publikováno v:
Nature communications, vol 11, iss 1
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
The creation of artificial enzymes is a key objective of computational protein design. Although de novo enzymes have been successfully designed, these exhibit low catalytic efficiencies, requiring directed evolution to improve activity. Here, we use
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27d365dfb9700690fb0ebca00b84ea69
https://escholarship.org/uc/item/0z88f0sj
https://escholarship.org/uc/item/0z88f0sj
Autor:
Michael C. Thompson, Mukhametzhanov N, Erin Nguyen, Lin Liu, Aron Broom, R.V. Rakotoharisoa, James S. Fraser, Roberto A. Chica, Zarifi N
The creation of artificial enzymes is a key objective of computational protein design. Although de novo enzymes have been successfully designed, these exhibit low catalytic efficiencies, requiring directed evolution to improve activity. Here, we used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff9f346c2710e9bb37961af65f018731
https://doi.org/10.1101/2020.03.19.999235
https://doi.org/10.1101/2020.03.19.999235
Publikováno v:
Communications Biology
Communications Biology, Vol 2, Iss 1, Pp 1-10 (2019)
Communications Biology, Vol 2, Iss 1, Pp 1-10 (2019)
Protein structures are dynamic, undergoing motions that can play a vital role in function. However, the link between primary sequence and conformational dynamics remains poorly understood. Here, we studied how conformational dynamics can arise in a g
Publikováno v:
Biochemistry. 56:2106-2115
Mobile Ω-loops play essential roles in the function of many enzymes. Here we investigated the importance of a residue lying outside of the mobile Ω-loop element in the catalytic function of an H477R variant of cytosolic phosphoenolpyruvate carboxyk
Publikováno v:
Structure. 28:717-726.e3
Accurate modeling of the effects of mutations on protein stability is central to understanding and controlling proteins in myriad natural and applied contexts. Here, we reveal through rigorous quantitative analysis that stability prediction tools oft
Publikováno v:
Protein Science. 24:580-587
Although the folding rates of proteins have been studied extensively, both experimentally and theoretically, and many native state topological parameters have been proposed to correlate with or predict these rates, unfolding rates have received much