Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Autor: Speight Robert, Arnold S Alison, Bergler Gabriele, Nahalka Jozef, Abad Sandra, Fotheringham Ian, Nidetzky Bernd, Glieder Anton

Publikováno v: Microbial Cell Factories, Vol 9, Iss 1, p 24 (2010)

Abstract Background Trigonopsis variabilis D-amino acid oxidase (TvDAO) is a well characterized enzyme used for cephalosporin C conversion on industrial scale. However, the demands on the enzyme with respect to activity, operational stability and cos

Externí odkaz: https://doaj.org/article/69b27c659d18494ca79bcfbe1dd876bc

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The fluorescent protein iLOV as a reporter for screening of high-yield production of antimicrobial peptides in Pichia pastoris

Autor: Kjeldsen, Annemette, Kay, Jack E., Baxter, Scott, McColm, Stephen, Serrano-Amatriain, Cristina, Parker, Scott, Robb, Ellis, Arnold, S. Alison, Gilmour, Craig, Raper, Anna, Robertson, Graeme, Fleming, Robert, Smith, Brian O., Fotheringham, Ian G., Christie, John M., Magneschi, Leonardo

Publikováno v: Kjeldsen, A, Kay, J E, Baxter, S, McColm, S, Serrano-Amatriain, C, Parker, S, Robb, E, Arnold, S A, Gilmour, C, Raper, A, Robertson, G, Fleming, R, Smith, B O, Fotheringham, I G, Christie, J M & Magneschi, L 2022, ' The fluorescent protein iLOV as a reporter for screening of high-yield production of antimicrobial peptides in Pichia pastoris ', Microbial biotechnology, vol. 15, no. 7, pp. 2126-2139 . https://doi.org/10.1111/1751-7915.14034

The methylotrophic yeast Pichia pastoris is commonly used for the production of recombinant proteins at scale. The identification of an optimally overexpressing strain following transformation can be time and reagent consuming. Fluorescent reporters

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e09871ace45658b4eb94f6975f60551
https://hdl.handle.net/20.500.11820/2f41e8ee-3413-41bc-b115-c00b3de36e57

Monitoring a high cell density recombinant Pichia pastoris fed-batch bioprocess using transmission and reflectance near infrared spectroscopy

Autor: Crowley, John, Arnold, S. Alison ^*, Wood, Nigel, Harvey, Linda M., McNeil, Brian

Publikováno v: In Enzyme and Microbial Technology 2005 36(5):621-628

Near Infrared Spectroscopy for Bioprocess Monitoring and Control: Current Status and Future Trends.

Autor: Scarff, Matthew¹ (AUTHOR), Arnold, S. Alison¹ (AUTHOR), Harvey, Linda M.¹ (AUTHOR), McNeil, Brian¹ (AUTHOR)

Publikováno v: Critical Reviews in Biotechnology. 2006, Vol. 26 Issue 1, p17-39. 23p. 3 Diagrams, 2 Charts.