Výsledky vyhledávání - "Arnold Horwitz"

Antibody engineering to improve manufacturability

Autor: Fangjiu Zhang, Fraser Conrad, Consuelo Garcia-Rodriquez, Sujeewa D. Wijesuriya, Milan T. Tomic, Shauna Farr-Jones, Elizabeth Pongo, James D. Marks, Arnold Horwitz

Publikováno v: Protein expression and purification. 149

Expression variation among antibodies produced by stably transfected Chinese Hamster Ovary (CHO) cells is well established. While developing CHO-K1 cell lines, we encountered a human monoclonal antibody, mAb B-c, with severe manufacturability issues,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf52096ce28267c3398fd4a7e0d45306
https://pubmed.ncbi.nlm.nih.gov/29655788

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Enhancement of antibody fragment secretion into the Escherichia coli periplasm by co-expression with the peptidyl prolyl isomerase, FkpA, in the cytoplasm

Autor: Lauren Schwimmer, Hassan Issafras, Hoa M. Giang, Chung-Leung Chan, David J. Bohmann, Toshihiko Takeuchi, Nithin B. Reddy, Raphael Levy, Kiran Ahluwalia, Arnold Horwitz

Publikováno v: Journal of Immunological Methods. 394(1-2):10-21

Improper protein folding or aggregation can frequently be responsible for low expression and poor functional activity of antibody fragments secreted into the Escherichia coli periplasm. Expression issues also can affect selection of antibody candidat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::beae410ee10e905080bf5fd67760b245

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XOMA 052, a potent, high-affinity monoclonal antibody for the treatment of IL-1β-mediated diseases

Autor: Paul D. Larsen, Alexander Owyang, Elizabeth Pongo, Marina Roell, Mary Haak-Frendscho, Masahisa Handa, Linda Masat, Hassan Issafras, Kiran Ahluwalia, John Corbin, Arnold Horwitz

Publikováno v: mAbs. 3:49-60

Interleukin-1β (IL-1β) is a potent mediator of inflammatory responses and plays a role in the differentiation of a number of lymphoid cells. In several inflammatory and autoimmune diseases, serum levels of IL-1β are elevated and correlate with dis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa69ccd08b967c0e590adbe4bb95db65
https://doi.org/10.4161/mabs.3.1.13989

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Kinetic Approach to Pathway Attenuation Using XOMA 052, a Regulatory Therapeutic Antibody That Modulates Interleukin-1β Activity

Publikováno v: Journal of Biological Chemistry. 285:20607-20614

Many therapeutic antibodies act as antagonists to competitively block cellular signaling pathways. We describe here an approach for the therapeutic use of monoclonal antibodies based on context-dependent attenuation to reduce pathologically high acti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77194264fd73d946d170c670a65fe525
https://doi.org/10.1074/jbc.m110.115790

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rBPI(10—193) is secreted by CHO cells and retains the activity of rBPI21

Autor: Rossana Nadell, Arnold Horwitz, Russell L. Dedrick, Robert E. Williams, W. Steve Ammons, Robert J. Bauer, Pei-Syan Liu

Publikováno v: Journal of Endotoxin Research. 10:97-106

rBPI23, a recombinant N-terminal fragment of human bactericidal/permeability-increasing protein (BPI), kills Gram-negative bacteria and neutralizes endotoxin. rBPI21, a variant in which cysteine 132 is changed to alanine, retains the activities of rB

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0fe53ae31aebeb5542a2774cd10279a5
https://doi.org/10.1177/09680519040100020501

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In Vitro and In Vivo Pharmacology and Pharmacokinetics of a Human Engineered™ Monoclonal Antibody to Epithelial Cell Adhesion Molecule

Autor: W. Steve Ammons, Harry H. Ruan, Robert J. Bauer, Kristen R. Scott, Marina Abramova, Eddie Bautista, Russell L. Dedrick, Arnold Horwitz, Zhi J. Chen

Publikováno v: Neoplasia: An International Journal for Oncology Research, Vol 5, Iss 2, Pp 146-154 (2003)

ING-1(heMAb), a Human Engineered monoclonal antibody to epithelial cell adhesion molecule (Ep-CAM), was evaluated for its in vitro and in vivo activity. The dissociation constant of ING-1(heMAb) for binding to Ep-CAM on HT-29 human colon tumor cells

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecffdb9636740e52520ef1ba13f25a70

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Biochemical Characterization of Recombinant Fusions of Lipopolysaccharide Binding Protein and Bactericidal/Permeability-increasing Protein

Autor: Nneka Ottah, Hsiu-Mei Wu, Georgia Theofan, Ken Der, Susan Abrahamson, Arnold Horwitz, Scott D. Leigh, Anne Orme, Russell L. Dedrick, Robert J. Bauer, Little Roger G, Helene Gazzano-Santoro, Stephen F. Carroll, Robert E. Williams

Publikováno v: Journal of Biological Chemistry. 272:2149-2155

The physiological response to endotoxin (lipopolysaccharide (LPS)) can be regulated by two closely related LPS-binding proteins, LPS-binding protein (LBP), which potentiates LPS' inflammatory activity via interaction with the monocytic antigen CD14,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::55792183e802ffa287618f72be4b1854
https://doi.org/10.1074/jbc.272.4.2149

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Functional premature polyadenylation signals and aberrant splicing within a recombinant protein coding sequence limit expression

Autor: Robyn Cotter, Sujeewa D. Wijesuriya, Arnold Horwitz

Publikováno v: Protein expression and purification. 92(1)

Recombinant glycoproteins can be produced at high levels in permanently transfected mammalian cells using expression vectors with strong viral promoters. CHO-K1 cell lines developed to produce the recombinant complement activator blocking protein, CA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6478ff7e8c5909283238aebb507a0a98
https://pubmed.ncbi.nlm.nih.gov/23994311

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