Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins

Autor: Alexander Pralow, Alexander Nikolay, Arnaud Leon, Yvonne Genzel, Erdmann Rapp, Udo Reichl

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)

Abstract Here, we present for the first time, a site-specific N-glycosylation analysis of proteins from a Brazilian Zika virus (ZIKV) strain. The virus was propagated with high yield in an embryo-derived stem cell line (EB66, Valneva SE), and concent

Externí odkaz: https://doaj.org/article/22d1a00117f2457d84ccc5377000bc89

The EB66® cell line as a valuable cell substrate for MVA-based vaccines production

Autor: Benoît Chatrenet, Brice Madeline, Matthias Hebben, Klaus Schwamborn, Arnaud Leon, Anne-Laure David, Thierry Huss, Patrick Champion-Arnaud, Elodie Dureau, Laurence Guianvarc’h

Publikováno v: Vaccine. 34:5878-5885

The selection of a cell substrate is a critical step for the development and manufacturing of a viral vaccine candidate. Several parameters such as cell susceptibility and permissiveness to the viral pathogens but also performance in terms of viral a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::401b21251570666716a4f09dd54b0050
https://doi.org/10.1016/j.vaccine.2016.10.043

Cell substrates for the production of viral vaccines

Autor: Fabien Perugi, Françoise Aubrit, Klaus Schwamborn, Patrick Champion-Arnaud, Arnaud Leon, Mehdi Lahmar, Fabienne Guehenneux

Publikováno v: Vaccine. 33:5905-5912

Vaccines have been used for centuries to protect people and animals against infectious diseases. For vaccine production, it has become evident that cell culture technology can be considered as a key milestone and has been the result of decades of pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5cbfafd77ef7d6562b0fc8b4c157beba
https://doi.org/10.1016/j.vaccine.2015.06.110

Tween-20 activates and solubilizes the mitochondrial membrane-bound, calmodulin dependent NAD+ finase of Avena sativa L

Autor: Danielle L. Laval-Martin, M. A. Pou de Crescenzo, Arnaud Leon, Stéphane Gallais

Publikováno v: Journal of Membrane Biology
Journal of Membrane Biology, Springer Verlag (Germany), 2001, 182 (2), pp.135-146. ⟨10.1007/s0023201-0039-8⟩
Journal of Membrane Biology, 2001, 182 (2), pp.135-146. ⟨10.1007/s0023201-0039-8⟩

International audience; Among different treatments assayed, a mix of a nonionic detergent (5% Tween-20) with 0.5 m NaCl was found to solubilize a large part of the calmodulin-dependent NAD+ kinase bound to the inner mitochondrial membrane. It also st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd73f3239d3b9f18dee817b912566561
https://pubmed.ncbi.nlm.nih.gov/11447505

Site-specific N-glycosylation analysis of animal cell culture-derived Zika virus proteins.

Autor: Pralow, Alexander¹, Nikolay, Alexander¹, Leon, Arnaud², Genzel, Yvonne¹, Rapp, Erdmann^1,3 rapp@mpi-magdeburg.mpg.de, Reichl, Udo^1,4

Publikováno v: Scientific Reports. 3/4/2021, Vol. 11 Issue 1, p1-7. 7p.