Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Arjen J Jakobi"'
Autor:
Daan B Boltje, Jacob P Hoogenboom, Arjen J Jakobi, Grant J Jensen, Caspar TH Jonker, Max J Kaag, Abraham J Koster, Mart GF Last, Cecilia de Agrela Pinto, Jürgen M Plitzko, Stefan Raunser, Sebastian Tacke, Zhexin Wang, Ernest B van der Wee, Roger Wepf, Sander den Hoedt
Publikováno v:
eLife, Vol 11 (2022)
Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used
Externí odkaz:
https://doaj.org/article/83cd08d1d010476f869e35008b71c55b
Publikováno v:
eLife, Vol 11 (2022)
Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier for routine high-resolu
Externí odkaz:
https://doaj.org/article/6102dea3224443ef8d777696f1e28eff
Publikováno v:
IUCrJ, Vol 11, Iss 6, Pp 951-965 (2024)
Conformational heterogeneity of biological macromolecules is a challenge in single-particle averaging (SPA). Current standard practice is to employ classification and filtering methods that may allow a discrete number of conformational states to be r
Externí odkaz:
https://doaj.org/article/6b34ad7c49d04d6f9fe41fef2b110fc4
Publikováno v:
eLife, Vol 6 (2017)
Atomic models based on high-resolution density maps are the ultimate result of the cryo-EM structure determination process. Here, we introduce a general procedure for local sharpening of cryo-EM density maps based on prior knowledge of an atomic refe
Externí odkaz:
https://doaj.org/article/98464d7c6d284435b28a920aad278cc0
Autor:
Matthias T Ehebauer, Michael Zimmermann, Arjen J Jakobi, Elke E Noens, Daniel Laubitz, Bogdan Cichocki, Hedia Marrakchi, Marie-Antoinette Lanéelle, Mamadou Daffé, Carsten Sachse, Andrzej Dziembowski, Uwe Sauer, Matthias Wilmanns
Publikováno v:
PLoS Pathogens, Vol 11, Iss 2, p e1004623 (2015)
Biotin-mediated carboxylation of short-chain fatty acid coenzyme A esters is a key step in lipid biosynthesis that is carried out by multienzyme complexes to extend fatty acids by one methylene group. Pathogenic mycobacteria have an unusually high re
Externí odkaz:
https://doaj.org/article/9a76312a97654e8193204c9108da9bfa
Autor:
Arjen J Jakobi, Eric G Huizinga
Publikováno v:
PLoS ONE, Vol 7, Iss 6, p e37617 (2012)
We describe a novel in vitro cloning strategy that combines standard tools in molecular biology with a basic protecting group concept to create a versatile framework for the rapid and seamless assembly of modular DNA building blocks into functional o
Externí odkaz:
https://doaj.org/article/b599adf1e91942e4af715ebd93a6a3b0
Autor:
Tanja Kuhm, Cecilia de Agrela Pinto, Luca Gross, Stefan T. Huber, Clémence Taisne, Evdokia A. Giannopoulou, Els Pardon, Jan Steyaert, Sander J. Tans, Arjen J. Jakobi
Guanylate-Binding Proteins (GBPs) are interferon-inducible guanosine triphosphate hydrolases (GTPases) that mediate immune effector functions against intracellular pathogens. A key step for the antimicrobial activity of GBPs is the formation of homo-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f53572f747faf58370e167de49f0a580
https://doi.org/10.1101/2023.03.28.534355
https://doi.org/10.1101/2023.03.28.534355
Autor:
Sharon Spizzichino, Federica Di Fonzo, Chiara Marabelli, Angela Tramonti, Antonio Chaves-Sanjuan, Alessia Parroni, Giovanna Boumis, Francesca Romana Liberati, Alessio Paone, Linda Celeste Montemiglio, Matteo Ardini, Arjen J. Jakobi, Alok Bharadwaj, Paolo Swuec, Gian Gaetano Tartaglia, Alessandro Paiardini, Roberto Contestabile, Serena Rinaldo, Martino Bolognesi, Giorgio Giardina, Francesca Cutruzzolà
SUMMARYRNA can directly control protein activity in a process called riboregulation; only a few mechanisms of riboregulation have been described in detail, none of these being characterized on structural grounds. Here we present a comprehensive struc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2f9f52133ee2417e922af975fd32e89a
https://doi.org/10.1101/2023.03.17.532973
https://doi.org/10.1101/2023.03.17.532973
Autor:
Saskia E. Bakker, Julien Bergeron, Alok Bharadwaj, David Bhella, Per Bullough, Pak-Lee Chau, René A. W. Frank, Arjen J. Jakobi, Agnel Praveen Joseph, Werner Kühlbrandt, Indrajit Lahiri, Rebbekah Menday, Stephen P. Muench, Mark Nakasone, Dmitry Nerukh, Giulia Paris, Christopher J. Russo, Helen R. Saibil, Sjors H. W. Scheres, Vidhi Sehrawat, Archna R. Shah, Andrea Thorn, José Luis Vilas, Giulia Zanetti
Publikováno v:
Faraday Discussions. 240:229-242
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ec0102663341ca4bfd6cd5f34a37b133
https://doi.org/10.1039/d2fd90061k
https://doi.org/10.1039/d2fd90061k
Autor:
Radim Skoupy, Daan B. Boltje, Miroslav Slouf, Katerina Mrazova, Tomas Laznicka, Clémence M. Taisne, Vladislav Krzyzanek, Jacob P. Hoogenboom, Arjen J. Jakobi
Publikováno v:
SMALL METHODS
A quantitative four-dimensional scanning transmission electron microscopy (4D-STEM) imaging technique (q4STEM) for local thickness estimation across amorphous specimen such as obtained by focused ion beam (FIB)-milling of lamellae for (cryo-)TEM anal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2d2f68ce25c2207b74c1c6f279595c2
http://resolver.tudelft.nl/uuid:8e0ac213-5f21-4550-9f77-7f03ec109e4c
http://resolver.tudelft.nl/uuid:8e0ac213-5f21-4550-9f77-7f03ec109e4c