Mass Spectrometric Detection of Cholesterol Oxidation in Bovine Sperm1

Autor: Renske A. van Gestel, Arjan Boerke, Jos F. Brouwers, Patricia F.N. Silva, Chris H.A. van de Lest, Barend M. Gadella, J. Bernd Helms, Nuria Garcia-Gil

Publikováno v: Biology of Reproduction. 85:128-136

We report on the presence and formation of cholesterol oxidation products (oxysterols) in bovine sperm. Although cholesterol is the most abundant molecule in the membrane of mammalian cells and is easily oxidized, this is the first report on choleste

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6ee3152e402b5060e1761b6ee7dbd10d
https://doi.org/10.1095/biolreprod.111.091207

Involvement of Bicarbonate-Induced Radical Signaling in Oxysterol Formation and Sterol Depletion of Capacitating Mammalian Sperm During In Vitro Fertilization1

Autor: J. Bernd Helms, Arjan Boerke, Edita Sostaric, Jos F. Brouwers, Chris H.A. van de Lest, Barend M. Gadella, E.J. Schoevers, Vesa M. Olkkonen

Publikováno v: Biology of Reproduction. 88

This study demonstrates for the first time that porcine and mouse sperm incubated in capacitation media supplemented with bicarbonate produce oxysterols. The production is dependent on a reactive oxygen species (ROS) signaling pathway that is activat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::60996d49c448a3c68d367e381a3402f9
https://doi.org/10.1095/biolreprod.112.101253

Thickness and morphology of polyelectrolyte coatings on silica surfaces before and after protein exposure studied by atomic force microscopy

Autor: Frits M. Flesch, Govert W. Somsen, Rob Haselberg, Arjan Boerke

Publikováno v: Haselberg, R, Flesch, F, Boerke, A & Somsen, G W 2013, ' Thickness and morphology of polyelectrolyte coatings on silica surfaces before and after protein exposure studied by atomic force microscopy ', Analytica Chimica Acta, vol. 779, pp. 90-95 . https://doi.org/10.1016/j.aca.2013.03.066
Analytica Chimica Acta, 779, 90-95. Elsevier

Analyte-wall interaction is a significant problem in capillary electrophoresis (CE) as it may compromise separation efficiencies and migration time repeatability. In CE, self-assembled polyelectrolyte multilayer films of Polybrene (PB) and dextran su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e153baedd07733a34b5a34641fe34145
https://pubmed.ncbi.nlm.nih.gov/23663676

Capacitation-dependent reorganization of microdomains in the apical sperm head plasma membrane: functional relationship with zona binding and the zona-induced acrosome reaction

Autor: Ian Andrew Brewis, Arjan Boerke, Pei-Shiue Tsai, Nuria Garcia-Gil, Bart M. Gadella

Publikováno v: Theriogenology. 70(8)

For sperm to successfully fertilize an oocyte, it needs to pass through certain steps prior to, during and after initial recognition of the zona pellucida (ZP). During capacitation, the surface of the sperm head becomes remodelled, priming it to bind

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5af9f88febb612eb807efce363728594
https://pubmed.ncbi.nlm.nih.gov/18640708

Bicarbonate-Induced Radical Signaling, Oxysterol Formation and Sterol Depletion in Capacitating Porcine Sperm

Autor: J. Bernd Helms, Arjan Boerke, Bart M. Gadella, Vesa M. Olkkonen, Jos F. Brouwers

Publikováno v: Biology of Reproduction. 87:359-359

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0fb8661730c9dd3730cf1a8423b0d86e
https://doi.org/10.1093/biolreprod/87.s1.359