Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases

Autor: Anu Koivula, Marika Alapuranen, Jarno Kallio, Jari Vehmaanperä, Arja Lappalainen, Sanni Voutilainen, Liisa Viikari, Matti Siika-aho, Satu Hooman, Terhi Puranen

Publikováno v: Voutilainen, S, Puranen, T, Siika-aho, M, Lappalainen, A, Alapuranen, M, Kallio, J, Hooman, S, Viikari, L, Vehmaanperä, J & Koivula, A 2008, ' Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases ', Biotechnology and Bioengineering, vol. 101, no. 3, pp. 515-528 . https://doi.org/10.1002/bit.21940

As part of the effort to find better cellulases for bioethanol production processes, we were looking for novel GH‐7 family cellobiohydrolases, which would be particularly active on insoluble polymeric substrates and participate in the rate‐limiti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd27cf77ec629f9428a927cd92e845ed
https://doi.org/10.1002/bit.21940

Purification and Characterization ofBacillus acidopullulyticus Pullulanase for Enzymatic Starch Modification

Autor: Tapani Suortti, Arja Lappalainen, Kaisa Poutanen, Marja-Leena Niku-Paavola

Publikováno v: Lappalainen, A, Niku-Paavola, M-L, Suortti, T & Poutanen, K 1991, ' Purification and characterization of Bacillus acidopullulyticus pullulanase for enzymatic starch modification ', Stärke, vol. 43, no. 12, pp. 477-482 . https://doi.org/10.1002/star.19910431207

The pullulanase (EC 3.2.1.41) of Bacillus acidopullulyticus was purified using anion exchange chromatography and preparative isoelectric focusing. The purified preparation migrated as a single protein band upon SDS gel electrophoresis and its molecul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c86aab612f04be9598156387d390b7b
https://doi.org/10.1002/star.19910431207

Endoxylanase II from Trichoderma reesei has several isoforms with different isoelectric points

Autor: Maija Tenkanen, Nisse Kalkkinen, Richard Fagerström, Matti Siika-aho, Arja Lappalainen

Publikováno v: Lappalainen, A, Siika-aho, M, Kalkkinen, N, Fagerström, R & Tenkanen, M 2000, ' Endoxylanase II from Trichoderma reesei has several isoforms with different isoelectric points ', Biotechnology and Applied Biochemistry, vol. 31, no. 1, pp. 61-68 . https://doi.org/10.1042/BA19990066
University of Helsinki

Two minor xylanases present in Trichoderma reesei Rut C30 cultivation broth were purified as a mixture using ion‐exchange, hydrophobic‐interaction and gel chromatography. The purified enzyme preparation contained two active xylanases with pI valu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c85d096bf4720ca62af73d29be46f549
https://cris.vtt.fi/en/publications/d69011d5-9e21-4c63-8c49-3d5f5e5fa25c

Immunochemical methods for chemical mapping of pulp fibres

Autor: Jaakko Pere, Arja Lappalainen, Maija Tenkanen

Publikováno v: Pere, J, Lappalainen, A & Tenkanen, M 1999, Immunochemical methods for chemical mapping of pulp fibres . in Microscopy as a Tool in Pulp and Paper Research and Development : EC Con-certed Action Symposium ., 90, Skogs-och träforskningsinstitutet, Stockholm, Microscopy as a Tool in Pulp and Paper Research and Development, Stockholm, Sweden, 21/06/99 .
University of Helsinki

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::026ad5bab570ca36f2cdf26dac28b707
https://cris.vtt.fi/en/publications/a0fa28d8-f4d9-4d65-a923-1b1f216b46e3

Hemicelluloses: Science and Technology

Autor: Paul Gatenholm, Maija Tenkanen, RunCang Sun, X. F. Sun, J. Tomkinson, Juergen Puls, Bodo Saake, P. Maréchal, J. Jorda, P.-Y. Pontalier, L. Rigal, B. Saake, N. Erasmy, Th. Kruse, E. Schmekal, J. Puls, H. Stålbrand, J. Lundqvist, A. Andersson, P. Hägglund, L. Anderson, F. Tjerneld, A. Jacobs, A. Teleman, O. Dahlman, M. Palm, G. Zacchi, Olof B. Dahlman, Anna Jacobs, Maria Nordström, Ursula Mais, Herbert Sixta, Mirjam A. Kabel, Henk A. Schols, Alphons G. J. Voragen, David J. Kiemle, Arthur J. Stipanovic, Kelly E. Mayo, Arja Lappalainen, Jaakko Pere, Robert H. Marchessault, Johannes P. Roubroeks, Wolfgang G. Glasser, Anne-Mari Olsson, Lennart Salmén, Alan Esker, Ulrike Becker, Sylvie Jamin, Shinji Beppu, Scott Renneckar, Wolfgang Glasser, Tea Hannuksela, Bjarne Holmbom, Åsa Linder, Per Tomas Larsson, Arja Paananen, Monika Österberg, Mark Rutland, Tekla Tammelin, Terhi Saarinen, Kirsi Tappura, Per Stenius, Thomas Heinze, Andreas Koschella, Anna Ebringerová, Charles M. Buchanan, Norma L. Buchanan, John S. Debenham, Maria Jacobsson, Michael C. Shelton, Thelma L.

Produced by the majority of trees and terrestrial plants, hemicelluloses (a set of polysaccharides) are the most abundant form of polymer after cellulose. Seeing hemicelluloses as an important yet neglected renewable resource that can move us beyond