Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Arik Dvir"'
Publikováno v:
Journal of Biological Chemistry. 278:10250-10256
Recent evidence, obtained in a reconstituted RNA polymerase II transcription system, indicated that the promoter escape stage of transcription requires template DNA located downstream of the elongating polymerase. In the absence of downstream DNA, ve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0d2e10b6fa1fde77fffe4b178905fa33
https://doi.org/10.1074/jbc.m210848200
https://doi.org/10.1074/jbc.m210848200
Publikováno v:
Proceedings of the National Academy of Sciences. 98:5544-5549
TFIIH is a multifunctional RNA polymerase II general initiation factor that includes two DNA helicases encoded by the Xeroderma pigmentosum complementation group B ( XPB ) and D ( XPD ) genes and a cyclin-dependent protein kinase encoded by the CDK7
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ca1cb92f0d978994daf61bedb3b5eab
https://doi.org/10.1073/pnas.101004498
https://doi.org/10.1073/pnas.101004498
Publikováno v:
Trends in Biochemical Sciences. 25:375-380
The elongation stage of eukaryotic mRNA synthesis can be regulated by transcription factors that interact directly with the RNA polymerase II (pol II) elongation complex and by activities that modulate the structure of its chromatin template. Recent
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73bb8e566cedc2ebb2427d699edc7485
https://doi.org/10.1016/s0968-0004(00)01615-7
https://doi.org/10.1016/s0968-0004(00)01615-7
Publikováno v:
Journal of Biological Chemistry. 272:28175-28178
Shortly after initiating promoter-specific transcription in vitro, mammalian RNA polymerase II becomes highly susceptible to arrest in a promoter-proximal region 9–13 base pairs downstream of the transcriptional start site (Dvir, A., Conaway, R. C.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::898cf1a5de7dbab2aae973711c37c53f
https://doi.org/10.1074/jbc.272.45.28175
https://doi.org/10.1074/jbc.272.45.28175
Publikováno v:
Methods. 12:192-202
With the availability of the general initiation factors (TFIIB, TFIID, TFIIE, TFIIF, and TFIIH), it is now possible to investigate aspects of the mechanism of eukaryotic messenger RNA synthesis in purified, reconstituted RNA polymerase II transcripti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87635ed2a41052c8d151361e5a61c798
https://doi.org/10.1006/meth.1997.0471
https://doi.org/10.1006/meth.1997.0471
Publikováno v:
Journal of Biological Chemistry. 268:10440-10447
We have recently shown that a template-associated protein kinase, which phosphorylates the carboxyl-terminal domain (CTD) of RNA polymerase II, is a two-component system. We describe here the purification of these two components to apparent homogenei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4c42c73e156471b15bf5150e6ca32ca3
https://doi.org/10.1016/s0021-9258(18)82219-0
https://doi.org/10.1016/s0021-9258(18)82219-0
Publikováno v:
Genes & Development. 6:426-438
Isolated transcription complexes contain a protein kinase that phosphorylates the heptapeptide repeats of the carboxy-terminal domain (CTD) of the RNA polymerase II (RNAP II) large subunit in an apparently promoter-dependent manner. We now show that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fbfa884305f5aafa46d03ffafc8a5ae
https://doi.org/10.1101/gad.6.3.426
https://doi.org/10.1101/gad.6.3.426
Autor:
Jennifer H. Gromek, Arik Dvir
Publikováno v:
Signal Transduction: Pathways, Mechanisms and Diseases ISBN: 9783642021114
Gene transcription is the most significant mechanism by which cells control the specific expression of their genes. In this review we will focus on the transcription of protein coding genes in eukaryotic cells. The transcription of these genes is cat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89623cf560851f6c4e1cfc137174628a
https://doi.org/10.1007/978-3-642-02112-1_14
https://doi.org/10.1007/978-3-642-02112-1_14
Publikováno v:
Methods in enzymology. 370
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c1f584bef3518e98b683b8483cc15054
https://pubmed.ncbi.nlm.nih.gov/14712687
https://pubmed.ncbi.nlm.nih.gov/14712687
Autor:
Woo J. Moon, Aaron J. McBride, Jessica A. Apostol, Zachary F. Burton, Arik Dvir, Lata I. Shukla
Publikováno v:
Protein expression and purification. 34(2)
Transcription factor IIE (TFIIE) is a general initiation and promoter escape factor for RNA polymerase II composed of p56 (TFIIE-alpha) and p34 (TFIIE-beta) subunits. Our laboratories experienced difficulty producing adequate quantities of recombinan
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ac7d3efac39c959e5633664261389e7
https://pubmed.ncbi.nlm.nih.gov/15003267
https://pubmed.ncbi.nlm.nih.gov/15003267