Direct Infusion Mass Spectrometry to Rapidly Map Metabolic Flux of Substrates Labeled with Stable Isotopes

Autor: Nils W. F. Meijer, Susan Zwakenberg, Johan Gerrits, Denise Westland, Arif I. Ardisasmita, Sabine A. Fuchs, Nanda M. Verhoeven-Duif, Judith J. M. Jans, Fried J. T. Zwartkruis

Publikováno v: Metabolites, Vol 14, Iss 5, p 246 (2024)

Direct infusion–high-resolution mass spectrometry (DI-HRMS) allows for rapid profiling of complex mixtures of metabolites in blood, cerebrospinal fluid, tissue samples and cultured cells. Here, we present a DI-HRMS method suitable for the rapid det

Externí odkaz: https://doaj.org/article/d7a1eb86047547ed993cb54f3f93b1c8

Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease

Autor: Monique M. A. Verstegen, Floris J. M. Roos, Ksenia Burka, Helmuth Gehart, Myrthe Jager, Maaike de Wolf, Marcel J. C. Bijvelds, Hugo R. de Jonge, Arif I. Ardisasmita, Nick A. van Huizen, Henk P. Roest, Jeroen de Jonge, Michael Koch, Francesco Pampaloni, Sabine A. Fuchs, Imre F. Schene, Theo M. Luider, Hubert P. J. van der Doef, Frank A. J. A. Bodewes, Ruben H. J. de Kleine, Bart Spee, Gert-Jan Kremers, Hans Clevers, Jan N. M. IJzermans, Edwin Cuppen, Luc J. W. van der Laan

Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)

Abstract The development, homeostasis, and repair of intrahepatic and extrahepatic bile ducts are thought to involve distinct mechanisms including proliferation and maturation of cholangiocyte and progenitor cells. This study aimed to characterize hu

Externí odkaz: https://doaj.org/article/eb9e7d98f7234f40b94f21db6d99f8db

Recapitulating Cholangiopathy-Associated Necroptotic Cell Death In Vitro Using Human Cholangiocyte Organoids

Autor: Floris J.M. Roos, Qiuwei Pan, Petra E. de Ruiter, Jan N. M. IJzermans, Arif I. Ardisasmita, Henk P. Roest, Shaojun Shi, Monique M A Verstegen, Luc J. W. van der Laan, Marije Niemeijer, Wanlu Cao

Publikováno v: Cellular and Molecular Gastroenterology and Hepatology, Vol 13, Iss 2, Pp 541-564 (2022)
Cellular and Molecular Gastroenterology and Hepatology
CMGH, 13(2), 541-564. Elsevier Inc.

Background & Aims Liver and bile duct diseases often are associated with extensive cell death of cholangiocytes. Necroptosis represents a common mode of programmed cell death in cholangiopathy, however, detailed mechanistic knowledge is limited owing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dd04ecf57291726ce9c8a99460e2895
https://doi.org/10.1016/j.jcmgh.2021.10.009