Discovery and characterization of non-canonical E2 conjugating enzymes

Autor: Syed Arif Abdul Rehman, Elena Di Nisio, Chiara Cazzaniga, Odetta Antico, Axel Knebel, Clare Johnson, Frederic Lamoliatte, Rodolfo Negri, Miratul Muqit MK, Virginia De Cesare

E2 conjugating enzymes (E2s) play a central role in the enzymatic cascade that leads to the attachment of ubiquitin to a substrate. This process, termed ubiquitylation is fundamental for maintaining cellular homeostasis and impacts almost all cellula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::5213529c293d7dae87454baf4c9f7290
https://doi.org/10.1101/2023.03.05.531151

Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2

Autor: Sven M. Lange, Syed Arif Abdul Rehman, Dmitri I. Svergun, Lee A. Armstrong, Yogesh Kulathu, Yosua Adi Kristariyanto, Axel Knebel, Tobias W. Gräwert

Publikováno v: 'Molecular Cell ', vol: 81, pages: 4176-4190 (2021)
Molecular cell 81, S1097276521006912 (1-15) (2021). doi:10.1016/j.molcel.2021.08.024
Molecular Cell

Summary Of the eight distinct polyubiquitin (polyUb) linkages that can be assembled, the roles of K48-linked polyUb (K48-polyUb) are the most established, with K48-polyUb modified proteins being targeted for degradation. MINDY1 and MINDY2 are members

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b23d77ec8d443d9dee20b6661b5b4428
https://epn-library.esrf.fr/flora/jsp/index_view_direct_anonymous.jsp?record=doc:PUB_ESRF:58802

SDS can be utilized as an amyloid inducer: a case study on diverse proteins.

Autor: Javed Masood Khan, Atiyatul Qadeer, Sumit Kumar Chaturvedi, Ejaz Ahmad, Syed Arif Abdul Rehman, Samudrala Gourinath, Rizwan Hasan Khan

Publikováno v: PLoS ONE, Vol 7, Iss 1, p e29694 (2012)

Sodium dodecyl sulphate (SDS), an anionic surfactant that mimics some characteristics of biological membrane has also been found to induce aggregation in proteins. The present study was carried out on 25 diverse proteins using circular dichroism, flu

Externí odkaz: https://doaj.org/article/d3be5b33ee1b4eeaabe84afa98d25c60

A single MIU motif of MINDY ‐1 recognizes K48‐linked polyubiquitin chains

Autor: Simone Weidlich, Yosua Adi Kristariyanto, Axel Knebel, Yogesh Kulathu, Syed Arif Abdul Rehman

Publikováno v: EMBO reports

The eight different types of ubiquitin (Ub) chains that can be formed play important roles in diverse cellular processes. Linkage‐selective recognition of Ub chains by Ub‐binding domain (UBD)‐containing proteins is central to coupling different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0368614887c35e8a54c8a8c95510701c

Characterization and functional insights into the Entamoeba histolytica pyridoxal kinase, an enzyme essential for its survival

Autor: Mohammad Farhan Ali, Syed Arif Abdul Rehman, K.F. Tarique, S. Devi, Samudrala Gourinath, Priya Tomar

Publikováno v: Journal of Structural Biology. 212:107645

Pyridoxal 5'-phosphate (PLP) is the active form of vitamin B6 and a cofactor for more than 140 enzymes. This coenzyme plays a pivotal role in catalysis of various enzymatic reactions that are critical for the survival of organisms. Entamoeba histolyt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d467310a1359ca7c452efae3792f38b5
https://doi.org/10.1016/j.jsb.2020.107645