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pro vyhledávání: '"Ariella Vishlizky"'
Autor:
Channa Shalitin, Ariella Vishlizky
Publikováno v:
Current Genetics. 9:107-111
Two micrometer minichromosomes from Saccharomyces cerevisiae were isolated without detergent using metrizamide gradients. 2 μm minichromosomes showed a lower density in metrizamide gradients relative to genomic chromatin. Our results suggest a lower
Autor:
Ariella Vishlizky, Channa Shalitin
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 782:328-330
A procedure has been developed for the isolation of intact yeast chromatin from yeast nuclei. Autodigestion of chromatin observed during nuclear preparation was inhibited by the addition of 5 mM spermidine. The procedure is useful for the analysis of